LOCUS       sequence-496769-        8063 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PBT2364 biosensor circuit in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8063)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAH107
FEATURES             Location/Qualifiers
     source          1..8063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             1463..2089
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1539..1558)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2254..2272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(2359..2361,2362..2364,2365..3078)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2404..2425)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3099..3126
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5106..5897,5898..5966))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5729..5748
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7258..7646
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7817..7926
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggcatgacgg ttcgtaacgt attagacgaa
     1021 agaggacgcg aactatgcgg tgaatattgc cgtttctacg atttgaaacg gacagggatg
     1081 ttcaagagtt ccgattatct gaaagatact cacccggatc tcgcacggtt ctttaatccg
     1141 aattatgctt tacgtcccat ttctacgacc tttaccgcta ccattatcaa cggcagtgag
     1201 tatcagaatc cgggatatta aattgtaaga cctatatacc caatcattat cggtacgata
     1261 atccatatag ccacgctatt tatcgtacca atgattgggg atattttcca cacctttcca
     1321 catttttcca cacctcccca ataatatcgt ttttttcaac tctgctgtta atctgtatct
     1381 tatcctttca gcatttcttt atatcctatt ggtatatcct ttgtatcgac tttatagcaa
     1441 atgtattttt aaatataaaa gtatgatgtc cagattagat aaaagtaaag tgattaacag
     1501 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca
     1561 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct
     1621 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg
     1681 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag
     1741 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac
     1801 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata
     1861 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca
     1921 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca
     1981 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt
     2041 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcttaat ggccacatcg
     2101 gttcctgcta gaaggcccat cctgacggat ggccttttgg tattcgagca ttacctcata
     2161 ctttggattt ttgttttgtt tgcaatggtt aatctattgt taaaatttaa agtttcactt
     2221 gaactttcaa ataatgttct tatatttgca gtgtccctat cagtgataga gagtaggatc
     2281 acacaacatt taaaaaataa cattatgaaa gcactcgaaa agagattcaa aggtaatatt
     2341 aacaataatt tattttcaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2401 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc
     2461 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2521 cccgtgccct ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg cttcgcccgc
     2581 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2641 caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag
     2701 ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg gcatcgactt caaggaggac
     2761 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaaggt ctatatcacg
     2821 gccgacaagc agaagaacgg catcaaggct aacttcaaga cccgccacaa cgttgaggac
     2881 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2941 ctgctgcccg acaaccacta cctgagccat cagtccaagc tgagcaaaga ccccaacgag
     3001 aagcgcgatc acatggtcct gaaggagagg gtgaccgccg ccgggattac acatgacatg
     3061 gacgagctgt acaagtaata gcgttcagct cgactgtgag aaggccatcc tgacggatgg
     3121 cctttttttt tcgttataac tagttgcgtt cctagccgct actagtgtaa ttgcctatct
     3181 tccagtgatg gaacagcatt tgtgcattgg ctgcaacaat cagccttact tgtgcctgtt
     3241 ctatttccga accgaccgct tgtatgaatg catcaaaatt cgttttctct acgttggatt
     3301 ccttgttgct catattgtga tgataatttc tacaaatata gtcattggta actatctatg
     3361 aaactgtttg atacttttat agttgattaa acttgttcat ggcatttgcc ttaatatcat
     3421 ccgctatgtc aatgtagggt ttcatagctt tgtagtcgct gtgtcccgtc catttcatga
     3481 ccacctgtgc cgggattccg agagccagcg cattgcagat gaatgtcctt tttcctgcat
     3541 gggtactgag caaagcgtat ttgtgtgtga cttcatcaat acgttcattt cccttgtagt
     3601 aggtttcccg tacaggctcg ttgatttctg ccagttcgcc cagctctttc aggtaatcgt
     3661 tcatcttctg gttgctgatg acgggcagag ccatgtaatt ctcgaaatgg atgtccttgt
     3721 atttgtccag tatggctttg ctgtatttgt tcagttcaat cgtcaggctg tcggcagtct
     3781 tgactgtggt tatttcgatg tggtcggact tcacatcgct tcttttcaga ttgcgaacat
     3841 ccgaataccg caaactcgta aagcagcaga acaggaaaac atcacgcaca cgttccaggt
     3901 attgcttatc cttgggtatc tggtagtctt tcagcttgtt cagttcatcc caagtcagga
     3961 agattacttt tttcgaggtg gttttcagtt tcggtttgaa cgtatcgtat gcaatgttct
     4021 gatgatgtcc tttcttgaag ctccagcgca ggaaccattt gaggaatccc atttgcttgc
     4081 cgatggtgct gtttctcata tccttggtgt cacgcaggaa gttgacgtat tcgttcaatc
     4141 caaactcgtt gaaatagttg aacgttgcat cctccttgaa ctctttgagg tggttcctca
     4201 ctgctgcaaa tttttcatag gtggatgccg tccagttatt ctggttaccg cactctttta
     4261 caaactcatc gaacacctcc caaaagctga caggggcttc ttccggctgt tcttcactgg
     4321 tgtctttcat tctcatgttg aaagcttcct tcaactgttg ggtcgttggc atgacctcct
     4381 gcacctcaaa ttccttgaaa atattctgga tttcggcata gtatttcagc aagtccgtat
     4441 tgatttcggc tgcactttgc tttagcttgt tggtacatcc gttctttacc cgctgcttat
     4501 ctgcatccca tttggctacg tcaatccggt agcccgttgt aaactcgata cgttggctgg
     4561 caaagatgac acgcatacgg atgggtacgt tctctacgat tggcacaccg ttctttttcc
     4621 ggctctccaa tgcaaaaatg atgttgcgct tgatattcat aattgggtgc gtttgaaatt
     4681 ctacacccaa atatacaccc aattattgag atagcaaaag acatttagaa acatttactt
     4741 ttactctata ttgtaattta cacttgatta tcagtcgttt gcagttttat gatattctgt
     4801 gaaagtataa gttcgagagc ctgtctctcc gcaaaaaacg ctgaaaatca gcagattgca
     4861 aaacaaacac cctgttttac acccaagaat gtaaagtcgg gtgtttttgt tttatttaag
     4921 ataatacaac cactacataa taaaagagta gcgatattaa aagaatccga tgagaaaaga
     4981 ctaatattta tctatccatt cagtttgatt tctcaggact ttacatcgtc ctgaaagtat
     5041 ttgttggtac cggtaccgag gacgcgacag ctgcagacgc gttatgagta aacttggtct
     5101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5221 ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca
     5281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatggcc gcgggattaa
     6001 aagtcgggga ttggtgaaca aaaaggtgtt tctctcttta agagaaatat cgttttgcta
     6061 aacagttgat attgaggtat cattttatcg taaaagacat ttttgctcaa caattgcttg
     6121 acggaaatca acaaatttta gcattttgta aaaaagtcgc tatataattt ggtgaattgg
     6181 agttattttc atatttttgc atcccgaaga gtttctctta aagagagaaa catcttttgc
     6241 ataccttttc cgaccgaatt tttatgtcgt aaagaggggc tttgcagggg gtggactcag
     6301 aaagatgaga atagatgact attgtagttg aaacacatag aaagttgctg atatacagac
     6361 cgatacgcat atcgggatga accatgagta cgttcttttc tcaaaaaaca taaatattcg
     6421 aaaagagatg caataaatta aggagaggtt ataatgaaca aagtaaatat aaaagatagt
     6481 caaaatttta ttacttcaaa atatcacata gaaaaaataa tgaattgcat aagtttagat
     6541 gaaaaagata acatctttga aataggtgca gggaaaggtc attttactgc tggattggta
     6601 aagagatgta attttgtaac ggcgatagaa attgattcta aattatgtga ggtaactcgt
     6661 aataagctct taaattatcc taactatcaa atagtaaatg atgatatact gaaatttaca
     6721 tttcctagcc acaatccata taaaatattt ggcaacatac cttacaacat aagcacaaat
     6781 ataattcgaa aaattgtttt tgaaagttca gccacaataa gttatttaat agtggaatat
     6841 ggttttgcta aaatgttatt agatacaaac agatcactag cattgctgtt aatggcagag
     6901 gtagatattt ctatattagc aaaaattcct aggtattatt tccatccaaa acctaaagtg
     6961 gatagcacat taattgtatt aaaaagaaag ccagcaaaaa tggcatttaa agagagaaaa
     7021 aaatatgaaa cttttgtaat gaaatgggtt aacaaagagt acgaaaaact gtttacaaaa
     7081 aatcaattta ataaagcttt aaaacatgcg agaatatatg atataaacaa tattagtttc
     7141 gaacaatttg tatcgctatt taatagttat aaaatattta acggctaaaa acaataggcc
     7201 acatgcaact gtaaatgttt acgcgggtac cgacaccgcg gtggagggga attcccatgt
     7261 cagccgttaa gtgttcctgt gtcactcaaa attgctttga gaggctctaa gggcttctca
     7321 gtgcgttaca tccctggctt gttgtccaca accgttaaac cttaaaagct ttaaaagcct
     7381 tatatattct tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt
     7441 tatattaatt ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac
     7501 gttagccatg agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct
     7561 tagtacgtta aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa
     7621 caggttgaac tgctgatctt cagatcctct acgccggacg catcgtggcc ggatcaattc
     7681 cgttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
     7741 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
     7801 tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct
     7861 tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct
     7921 ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa
     7981 ccaggaaggg cagcccacct atcacggaat tgatccccct cgaattggaa ttctagcctg
     8041 gaatagggaa gcaccacatc acc
//
