LOCUS       sequence-496780-        7147 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  pTetR56 driving sfGFP in pNBU2 integrative Bacteroides plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7147)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM405
FEATURES             Location/Qualifiers
     source          1..7147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..44)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             251..874
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESET"
     primer_bind     complement(324..343)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(998..1041)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1000..1081
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1255..1273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1364..2077
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2084..2155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             2159..2176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2199..2226
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(4187..4978,4979..5047))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4810..4829
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6339..6727
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            6898..7007
                     /note="incP origin of transfer"
ORIGIN
        1 gataaaacga aaggctcagt cgaaagactg ggcctttcgt tttacaattg ggctaccttt
       61 tttttgtttt gtttgcaatg gttaatctat tgttaaaatt taaagtttca cttgaacttt
      121 caaataatgt tcttatattt ccagtgtcga aagaaacaaa gtaggactga tcacacaaca
      181 tttaaaaaat aacattatga aagcactcga aaagagattc aaaggtaata ttaacaataa
      241 tttattttca atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct
      301 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
      361 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
      421 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
      481 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
      541 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
      601 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
      661 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
      721 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
      781 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
      841 agaaaaacaa cttaaatgtg aaagtgagac gtaagtggag tgaccttaaa tcaaggacga
      901 tattaatcgg aaggagtatt caacgtgatg aagtcgcagg gttgacgtgg gacgtctctc
      961 ggtataacgc cggaaccgtc ccccgctaga ggcatcaata aaacgaaagg ctcagtcgaa
     1021 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1081 tgcggacaat aaaggatcgt cagaaggtaa cctgttatac aacgggcgga caataaagga
     1141 tcgtcagaag gtaacctgtt atacaacggt tttgttttgt ttgcaatggt taatctattg
     1201 ttaaaattta aagtttcact tgaactttca aataatgttc ttatatttgc agtgtcccta
     1261 tcagtgatag agagtaggac tgatcacaca acatttaaaa aataacatta tgaaagcact
     1321 cgaaaagaga ttcaaaggta atattaacaa taatttattt tcaatgcgta aaggcgaaga
     1381 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
     1441 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
     1501 catctgtact actggtaaac tgccggttcc ttggccgact ctggtaacga cgctgactta
     1561 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
     1621 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
     1681 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
     1741 aggcattgac tttaaagaag acggcaatat cctgggccat aagctggaat acaattttaa
     1801 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
     1861 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
     1921 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
     1981 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
     2041 agcgggcatc acgcatggta tggatgaact gtacaaagga tctgattaca aggacgatga
     2101 tgataaggac tacaaagatg atgatgacaa agattataag gacgatgatg ataaaggtca
     2161 tcatcatcat catcactaaa tcctagctga ttagctcgag aaggccatcc tgacggatgg
     2221 cctttttttt gtatcgagac gactagtgta attgcctatc ttccagtgat ggaacagcat
     2281 ttgtgcattg gctgcaacaa tcagccttac ttgtgcctgt tctatttccg aaccgaccgc
     2341 ttgtatgaat gcatcaaaat tcgttttctc tacgttggat tccttgttgc tcatattgtg
     2401 atgataattt ctacaaatat agtcattggt aactatctat gaaactgttt gatactttta
     2461 tagttgatta aacttgttca tggcatttgc cttaatatca tccgctatgt caatgtaggg
     2521 tttcatagct ttgtagtcgc tgtgtcccgt ccatttcatg accacctgtg ccgggattcc
     2581 gagagccagc gcattgcaga tgaatgtcct ttttcctgca tgggtactga gcaaagcgta
     2641 tttgggtgtg acttcatcaa tacgttcatt tcccttgtag taggtttccc gtacaggctc
     2701 gttgatttct gccagttcgc ccagctcttt caggtaatcg ttcatcttct ggttgctgat
     2761 gacgggcaga gccatgtaat tctcgaaatg gatgtccttg tatttgtcca gtatggcttt
     2821 gctgtatttg ttcagttcaa tcgtcaggct gtcggcagtc ttgactgtgg ttatttcgat
     2881 gtggtcggac ttcacatcgc ttcttttcag attgcgaaca tccgaatacc gcaaactcgt
     2941 aaagcagcag aacaggaaaa catcacgcac acgttccagg tattgcttat ccttgggtat
     3001 ctggtagtct ttcagcttgt tcagttcatc ccaagtcagg aagattactt ttttcgaggt
     3061 ggttttcagt ttcggtttga acgtatcgta tgcaatgttc tgatgatgtc ctttcttgaa
     3121 gctccagcgc aggaaccatt tgaggaatcc catttgcttg ccgatggtgc tgtttctcat
     3181 atccttggtg tcacgcagga agttgacgta ttcgttcaat ccaaactcgt tgaaatagtt
     3241 gaacgttgca tcctccttga actctttgag gtggttcctc actgctgcaa atttttcata
     3301 ggtggatgcc gtccagttat tctggttacc gcactctttt acaaactcat cgaacacctc
     3361 ccaaaagctg acaggggctt cttccggctg ttcttcactg gtgtctttca ttctcatgtt
     3421 gaaagcttcc ttcaactgtt gggtcgttgg catgacctcc tgcacctcaa attccttgaa
     3481 aatattctgg atttcggcat agtatttcag caagtccgta ttgatttcgg ctgcactttg
     3541 ctttagcttg ttggtacatc cgttctttac ccgctgctta tctgcatccc atttggctac
     3601 gtcaatccgg tagcccgttg taaactcgat acgttggctg gcaaagatga cacgcatacg
     3661 gatgggtacg ttctctacga ttggcacacc gttctttttc cggctctcca atgcaaaaat
     3721 gatgttgcgc ttgatattca taattgggtg cgtttgaaat tctacaccca aatatacacc
     3781 caattattga gatagcaaaa gacatttaga aacatttact tttactctat attgtaattt
     3841 acacttgatt atcagtcgtt tgcagtttta tgatattctg tgaaagtata agttcgagag
     3901 cctgtctctc cgcaaaaaac gctgaaaatc agcagattgc aaaacaaaca ccctgtttta
     3961 cacccaagaa tgtaaagtcg ggtgtttttg ttttatttaa gataatacaa ccactacata
     4021 ataaaagagt agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat
     4081 tcagtttgat ttctcaggac tttacatcgt cctgaaagta tttgttggta ccggtaccga
     4141 ggacgcgaca gctgcagacg cgttatgagt aaacttggtc tgacagttac caatgcttaa
     4201 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4261 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4321 taccgcggga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4381 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4441 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4501 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4561 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4621 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4681 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4741 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4801 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4861 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4921 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4981 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5041 tactcatact cttccttttt caatcatggc cgcgggatta aaagtcgggg attggtgaac
     5101 aaaaaggtgt ttctctcttt aagagaaata tcgttttgct aaacagttga tattgaggta
     5161 tcattttatc gtaaaagaca tttttgctca acaattgctt gacggaaatc aacaaatttt
     5221 agcattttgt aaaaaagtcg ctatataatt tggtgaattg gagttatttt catatttttg
     5281 catcccgaag agtttctctt aaagagagaa acatcttttg catacctttt ccgaccgaat
     5341 ttttatgtcg taaagagggg ctttgcaggg ggtggactca gaaagatgag aatagatgac
     5401 tattgtagtt gaaacacata gaaagttgct gatatacaga ccgatacgca tatcgggatg
     5461 aaccatgagt acgttctttt ctcaaaaaac ataaatattc gaaaagagat gcaataaatt
     5521 aaggagaggt tataatgaac aaagtaaata taaaagatag tcaaaatttt attacttcaa
     5581 aatatcacat agaaaaaata atgaattgca taagtttaga tgaaaaagat aacatctttg
     5641 aaataggtgc agggaaaggt cattttactg ctggattggt aaagagatgt aattttgtaa
     5701 cggcgataga aattgattct aaattatgtg aggtaactcg taataagctc ttaaattatc
     5761 ctaactatca aatagtaaat gatgatatac tgaaatttac atttcctagc cacaatccat
     5821 ataaaatatt tggcagcata ccttacaaca taagcacaaa tataattcga aaaattgttt
     5881 ttgaaagttc agccacaata agttatttaa tagtggaata tggttttgct aaaatgttat
     5941 tagatacaaa cagatcacta gcattgctgt taatggcaga ggtagatatt tctatattag
     6001 caaaaattcc taggtattat ttccatccaa aacctaaagt ggatagcaca ttaattgtat
     6061 taaaaagaaa gccagcaaaa atggcattta aagagagaaa aaaatatgaa acttttgtaa
     6121 tgaaatgggt taacaaagag tacgaaaaac tgtttacaaa aaatcaattt aataaagctt
     6181 taaaacatgc gagaatatat gatataaaca atattagttt cgaacaattt gtatcgctat
     6241 ttaatagtta taaaatattt aacggctaaa aacaataggc cacatgcaac tgtaaatgtt
     6301 tacgcgggta ccgacaccgc ggtggagggg aattcccatg tcagccgtta agtgttcctg
     6361 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     6421 tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc ttttttttct
     6481 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     6541 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     6601 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     6661 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     6721 tcagatcctc tacgccggac gcatcgtggc cggatcaatt ccgttttccg ctgcataacc
     6781 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     6841 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     6901 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     6961 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta ccgccggcgt
     7021 aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg gcagcccacc
     7081 tatcacggaa ttgatccccc tcgaattgga attcgttcct cgattcagat ctaaaggatc
     7141 caatcta
//
