LOCUS       sequence-496784-        8722 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  PopuA+10xTetO biosensor cicuit in pNBU2 integrative Bacteroides 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8722)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJB157
FEATURES             Location/Qualifiers
     source          1..8722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             207..893
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     complement(337..355)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1473..1544
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1560..1587
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2125..2748
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2198..2217)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2913..2931
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             join(3018..3020,3021..3023,3024..3737)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(3063..3084)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      3758..3785
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(join(5765..6556,6557..6625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6388..6407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7917..8305
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8476..8585
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1561 gctcaccttc gggtgggcct ttctgcgttt atatatgatg tgtaatgaca attatccttc
     1621 cccaggaaaa ttgtaatagc cctgttgaac atataaatct agtttagtta ttgacgccaa
     1681 atatacggtg tttaagtgaa atattcaaac ttattttata tttatctgcc ggtttgttct
     1741 attttgctga gtgatagggg aatattaccc ggaaactttc catatcatta caaataaagc
     1801 cctctgtatg ccccggattt tgaatataat ttgtatgttt aatccgtttt ttgtatcttt
     1861 gtgccagcaa gatactggaa caggctgttt ttatacataa aaatgctatt ttttgctatt
     1921 cgttgaaagg attatcttat caaattctat tcctttttta attactcagt ctgtcaataa
     1981 tcattttatt aattatttcg ggattcattg ttatttgtcc cttatttaaa tcagaacttt
     2041 atacgatata aagaaagatt cctgttatcg tttgttcttg tcttgctgtg tatattattt
     2101 taataaccta aaccatttta ttgtatgtcc agattagata aaagtaaagt gattaacagc
     2161 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     2221 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     2281 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     2341 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     2401 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     2461 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     2521 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     2581 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     2641 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     2701 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     2761 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     2821 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     2881 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     2941 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     3001 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3061 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     3121 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3181 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     3241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3301 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     3361 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     3421 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     3481 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     3541 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3601 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     3661 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     3721 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     3781 cttttttttt cgttataact agttgcgttc ctagccgcta ctagtgtaat tgcctatctt
     3841 ccagtgatgg aacagcattt gtgcattggc tgcaacaatc agccttactt gtgcctgttc
     3901 tatttccgaa ccgaccgctt gtatgaatgc atcaaaattc gttttctcta cgttggattc
     3961 cttgttgctc atattgtgat gataatttct acaaatatag tcattggtaa ctatctatga
     4021 aactgtttga tacttttata gttgattaaa cttgttcatg gcatttgcct taatatcatc
     4081 cgctatgtca atgtagggtt tcatagcttt gtagtcgctg tgtcccgtcc atttcatgac
     4141 cacctgtgcc gggattccga gagccagcgc attgcagatg aatgtccttt ttcctgcatg
     4201 ggtactgagc aaagcgtatt tgtgtgtgac ttcatcaata cgttcatttc ccttgtagta
     4261 ggtttcccgt acaggctcgt tgatttctgc cagttcgccc agctctttca ggtaatcgtt
     4321 catcttctgg ttgctgatga cgggcagagc catgtaattc tcgaaatgga tgtccttgta
     4381 tttgtccagt atggctttgc tgtatttgtt cagttcaatc gtcaggctgt cggcagtctt
     4441 gactgtggtt atttcgatgt ggtcggactt cacatcgctt cttttcagat tgcgaacatc
     4501 cgaataccgc aaactcgtaa agcagcagaa caggaaaaca tcacgcacac gttccaggta
     4561 ttgcttatcc ttgggtatct ggtagtcttt cagcttgttc agttcatccc aagtcaggaa
     4621 gattactttt ttcgaggtgg ttttcagttt cggtttgaac gtatcgtatg caatgttctg
     4681 atgatgtcct ttcttgaagc tccagcgcag gaaccatttg aggaatccca tttgcttgcc
     4741 gatggtgctg tttctcatat ccttggtgtc acgcaggaag ttgacgtatt cgttcaatcc
     4801 aaactcgttg aaatagttga acgttgcatc ctccttgaac tctttgaggt ggttcctcac
     4861 tgctgcaaat ttttcatagg tggatgccgt ccagttattc tggttaccgc actcttttac
     4921 aaactcatcg aacacctccc aaaagctgac aggggcttct tccggctgtt cttcactggt
     4981 gtctttcatt ctcatgttga aagcttcctt caactgttgg gtcgttggca tgacctcctg
     5041 cacctcaaat tccttgaaaa tattctggat ttcggcatag tatttcagca agtccgtatt
     5101 gatttcggct gcactttgct ttagcttgtt ggtacatccg ttctttaccc gctgcttatc
     5161 tgcatcccat ttggctacgt caatccggta gcccgttgta aactcgatac gttggctggc
     5221 aaagatgaca cgcatacgga tgggtacgtt ctctacgatt ggcacaccgt tctttttccg
     5281 gctctccaat gcaaaaatga tgttgcgctt gatattcata attgggtgcg tttgaaattc
     5341 tacacccaaa tatacaccca attattgaga tagcaaaaga catttagaaa catttacttt
     5401 tactctatat tgtaatttac acttgattat cagtcgtttg cagttttatg atattctgtg
     5461 aaagtataag ttcgagagcc tgtctctccg caaaaaacgc tgaaaatcag cagattgcaa
     5521 aacaaacacc ctgttttaca cccaagaatg taaagtcggg tgtttttgtt ttatttaaga
     5581 taatacaacc actacataat aaaagagtag cgatattaaa agaatccgat gagaaaagac
     5641 taatatttat ctatccattc agtttgattt ctcaggactt tacatcgtcc tgaaagtatt
     5701 tgttggtacc ggtaccgagg acgcgacagc tgcagacgcg ttatgagtaa acttggtctg
     5761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5881 gccccagtgc tgcaatgata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     5941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6601 cgacacggaa atgttgaata ctcatactct tcctttttca atcatggccg cgggattaaa
     6661 agtcggggat tggtgaacaa aaaggtgttt ctctctttaa gagaaatatc gttttgctaa
     6721 acagttgata ttgaggtatc attttatcgt aaaagacatt tttgctcaac aattgcttga
     6781 cggaaatcaa caaattttag cattttgtaa aaaagtcgct atataatttg gtgaattgga
     6841 gttattttca tatttttgca tcccgaagag tttctcttaa agagagaaac atcttttgca
     6901 taccttttcc gaccgaattt ttatgtcgta aagaggggct ttgcaggggg tggactcaga
     6961 aagatgagaa tagatgacta ttgtagttga aacacataga aagttgctga tatacagacc
     7021 gatacgcata tcgggatgaa ccatgagtac gttcttttct caaaaaacat aaatattcga
     7081 aaagagatgc aataaattaa ggagaggtta taatgaacaa agtaaatata aaagatagtc
     7141 aaaattttat tacttcaaaa tatcacatag aaaaaataat gaattgcata agtttagatg
     7201 aaaaagataa catctttgaa ataggtgcag ggaaaggtca ttttactgct ggattggtaa
     7261 agagatgtaa ttttgtaacg gcgatagaaa ttgattctaa attatgtgag gtaactcgta
     7321 ataagctctt aaattatcct aactatcaaa tagtaaatga tgatatactg aaatttacat
     7381 ttcctagcca caatccatat aaaatatttg gcaacatacc ttacaacata agcacaaata
     7441 taattcgaaa aattgttttt gaaagttcag ccacaataag ttatttaata gtggaatatg
     7501 gttttgctaa aatgttatta gatacaaaca gatcactagc attgctgtta atggcagagg
     7561 tagatatttc tatattagca aaaattccta ggtattattt ccatccaaaa cctaaagtgg
     7621 atagcacatt aattgtatta aaaagaaagc cagcaaaaat ggcatttaaa gagagaaaaa
     7681 aatatgaaac ttttgtaatg aaatgggtta acaaagagta cgaaaaactg tttacaaaaa
     7741 atcaatttaa taaagcttta aaacatgcga gaatatatga tataaacaat attagtttcg
     7801 aacaatttgt atcgctattt aatagttata aaatatttaa cggctaaaaa caataggcca
     7861 catgcaactg taaatgttta cgcgggtacc gacaccgcgg tggaggggaa ttcccatgtc
     7921 agccgttaag tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag
     7981 tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt
     8041 atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt
     8101 atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     8161 ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt
     8221 agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac
     8281 aggttgaact gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcaattcc
     8341 gttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg tatatccatc
     8401 ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc cttggtgtat
     8461 ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg ggtgttcctt
     8521 cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc tgcgaggctg
     8581 gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa ccaagccaac
     8641 caggaagggc agcccaccta tcacggaatt gatccccctc gaattggaat tctagcctgg
     8701 aatagggaag caccacatca cc
//
