LOCUS       sequence-496785-        3598 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  LacI part vector for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3598)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM113
FEATURES             Location/Qualifiers
     source          1..3598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..77)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             293..1372
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLEET"
     primer_bind     complement(312..331)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     terminator      1473..1559
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1620..1637)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1791..2379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1871..1890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2562..2656)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2677..3336)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3251..3270
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3498..3516
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3520..3563)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3566..3585
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 gttcctcgat tcagatctaa aggatccaat ctagataaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttacaa ttgggctacc ttttttttgt tttgtttgca atggttaatc
      121 tattgttaaa atttaaagtt tcacttgaac tttcaaataa tgttcttata tttccagtgt
      181 cgaaagaaac aaagtaggac tgatcacaca acatttaaaa aataacatta tgaaagcact
      241 cgaaaagaga ttcaaaggta atattaacaa taatttattt tcaatggtga atgtgaaacc
      301 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      361 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      421 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      481 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      541 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      601 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      661 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      721 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      781 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      841 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      901 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
      961 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1021 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1081 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1141 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1201 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc cagtttcact
     1261 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1321 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaagagacgt aagtggagtg
     1381 accttaaatc aaggacgata ttaatcggaa ggagtattca acgtgatgaa gtcgcagggt
     1441 tgacgtggga cgtctctgat cggatctcca ggcatataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1561 ccaaagcact gctcgctttg ttaagacggt gagaccttac ttcgcgttat gcaggcttcc
     1621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     1801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2521 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
     2581 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     2641 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     2701 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     2761 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     2821 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     2881 actcacccag ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata
     2941 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     3001 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     3061 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     3121 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     3181 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     3241 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     3301 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     3361 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     3421 acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca
     3481 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     3541 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagaatct aggtctcc
//
