LOCUS       sequence-496786-        3167 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  TetR part vector for Golden Gate Assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  TITLE     Tropini Lab plasmids
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEM111
FEATURES             Location/Qualifiers
     source          1..3167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..77)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             284..907
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESET"
     primer_bind     complement(357..376)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(1031..1074)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1033..1114
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1189..1206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1360..1948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1440..1459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2131..2225)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2246..2905)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2820..2839
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3067..3085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3089..3132)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3135..3154
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 gttcctcgat tcagatctaa aggatccaat ctagataaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttacaa ttgggctacc ttttttttgt tttgtttgca atggttaatc
      121 tattgttaaa atttaaagtt tcacttgaac tttcaaataa tgttcttata tttccagtgt
      181 cgaaagaaac aaagtaggac tgatcacaca acatttaaaa aataacatta tgaaagcact
      241 cgaaaagaga ttcaaaggta atattaacaa taatttattt tcaatgtcca gattagataa
      301 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
      361 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
      421 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
      481 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
      541 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
      601 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
      661 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
      721 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
      781 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
      841 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtga
      901 gacgtaagtg gagtgacctt aaatcaagga cgatattaat cggaaggagt attcaacgtg
      961 atgaagtcgc agggttgacg tgggacgtct ctcggtataa cgccggaacc gtcccccgct
     1021 agaggcatca ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1081 tttgtcggtg aacgctctcc tgagtaggac aaatgcggac aataaaggat cgtcagaagg
     1141 taacctgtta tacaacggtg agaccttact tcgcgttatg caggcttcct cgctcactga
     1201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
     1381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1801 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2101 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
     2161 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     2221 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     2281 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     2341 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     2401 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     2461 gattggctga cacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     2521 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     2581 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     2641 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     2701 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     2761 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2821 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2881 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2941 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     3001 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3061 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     3121 aaggatgatt tctggaattc gcggccgctt ctagaatcta ggtctcc
//
