LOCUS       sequence-496830-       15151 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  BglBrick plasmid 
            (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV 
            pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pJBEI-6410
FEATURES             Location/Qualifiers
     source          1..15151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(708..735)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(751..822)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3437..3453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3461..3467,3468..3484,3485..3490))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(5188..5199)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    8222..8238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8246..8252,8253..8269,8270..8275))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      complement(8488..8515)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(8531..8602)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             11604..11615
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    12350..12366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12374..12380,12381..12398,12399..12404))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(12490..12509)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        12715..12792
                     /gene="lacI"
                     /label=lacI promoter
     CDS             12793..13875
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(12812..12831)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        13949..14053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14054..14122,14123..14914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14272..14291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      14944..15038
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatccctag gtataaacgc agaaaggccc
      721 acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa
      781 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg agatccttac
      841 tcgagtttgg atccttaggc gaaaggtgca aacagggtac gcgtcatctg ctgatgaata
      901 ataggatgct gggtcccatg accatcaccg ttatgataca tcagctgggc catacgaccc
      961 agatcaacgg cgcaaccaat aaaatcctta ccaaacggac tatccttgga aacacgttca
     1021 gcgttcatct tcttccacac ttcggcgatc agccacttca catgtttgcg agcttcagct
     1081 tcacttgcgt tataatcgga catgtagcac tgtaaggatt tcggaacatc cccacgagac
     1141 acctcttcca cagacgtacc cagatcgtcc gccagccgca ggacgaaaga tgaccaacga
     1201 accaggtcat ggtatttata taaggaatcg accgtctcct tggtaaatga atccgtgacg
     1261 cgaaagaaga tatgcgtcag catgcatggc ccactgatac tctgccatga gttctccaga
     1321 tattcttcta aactcggttt gtgcccaccg tagaaccagc gtgcctcaac catatattta
     1381 tcggccagat ccacccaact ctgacgcaga taagggataa cattcacgcc tttttctttc
     1441 atgacgtcat aagaggtgtc gtccacaaag ttattcagcg ccagaaaaca cagctgcatg
     1501 taatctggca gctggtcgat gctattgatg tcccaccggc ggattaaatc tgtaaactgc
     1561 tccagttctt ccagagtgcc gtatacatcg taaatatcgt caatgacagt aatcagcgca
     1621 ttgaccttac ccatcataat acgggcgctc gcgtgctggc gcggttcgat aatgccggta
     1681 ttccagaaat aacattcaac cagacgatcg cgcgcaaacg gtaatttttc aacgaagccg
     1741 gtgttgcgcc accagcgaaa agattctttc agttcttcct gaaattgagc ctgcacgatg
     1801 tttaagtcta aaattgctaa ttccagcacc accgggttca tgtctggacg tttgcgatac
     1861 cactcaatcc acaccggcgc gtttggccgt ttaatacgcc agtgtaacgg aatgtccaga
     1921 gaatacgcga tgcgggttaa cagatcgcca tctacgccgc cttcatttac tttctcttct
     1981 aagaatttgg tcgcaaattc ccgtgcgctt tccagagtcg tctccccctc ggtcagcaga
     2041 aagctcgctt catacagttg taataagcca cgagtatcat cggacagtga ttctttgaac
     2101 tcgccctctt cattcttgaa gctatcgaat acctcttgtg ctacttggaa gccatgctca
     2161 cgcagtaacc gaaaggccag gcttgtgcta tacagatcac gttcctcttt cggaaaaggg
     2221 tttttatagt aatggtggtc cagatagata gatgacagaa tctctttgaa ctcattctga
     2281 aagtgatcgc tcagccccat gcgctgtaag tcgtcaatca gctctaattg gcgaatttgg
     2341 tctgtttctt tttccagttc cattttaacc agtgtaacca gttcgcttgc ccgaatgacg
     2401 tgtttgtcct ctttgtaatc gctcagcagg gattggataa aattcacatc ccaacgtgac
     2461 gggttgtaat taccactgcg acgcatgtat atctccttct taaaagtacc ttaattctga
     2521 cgaaatgcca cgtagtctgc caggcccagc agcggagcgg ctttaactgg gtcgaagcag
     2581 gacagttcac ccttcgcacg gttcagcagt tcatcggaga actccttggc cttttccaga
     2641 cccatcagct ttgggtaggt ggctttatca gagatcaggt ccttgcccgc agtcttaccc
     2701 agttcgtcgc tgcttttagt tacgtccaga atgtcatcaa ctacctggaa cagcagacca
     2761 acacagcggg cgtagcgacg ggcacgttcg attacgattt cgctggcacc gccaatgatt
     2821 gcaccgcaaa caacggagca ttccagcagc attgcggttt tgtggatgtg aatccattcc
     2881 agggtctgca ggtcgataga cggatcgcct tcggatgcga tgtctaccat ctgaccaccc
     2941 ataacacctt cagagccagt agcacgaccc agctcggaca ccatacgcag gatgcggtca
     3001 gcaccaacgg ttttagaagt agaaaccgcg atatgctcga aggcgtaaga gtgcagtgcg
     3061 ttaccagcag tcactgcggt atcctcaccg aaaattttgt ggttagtcgg tttaccgcga
     3121 cgcagatcgt cgttgtcgat acacggcaga tcatcgtgca tcagggacat cgtgtggatc
     3181 atttcaattg cgcacgcggt cgggatcgcc agttcttcgg taccacctac cagttcacat
     3241 gcggcaatac acagaaccgg acgaacacgc ttgccgcctg ccagcaggct gtaacgcatg
     3301 ctttcgtaga ttttctgcgg ataacgcaga gggatcgctt tgttcagtgc ttcatttacc
     3361 gtcatcgctt tggagtccat gtatttgttg aagtcaaatt ccatatgtat atctccttct
     3421 ttaaaagatc ctgaaattgt tatccgctca caattccaca cattatacga gccggatgat
     3481 taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa
     3541 acattatcca gaacgggagt gcgccttgag cgacacgaat tatgcagtga tttacgacct
     3601 gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc
     3661 agtcggcgaa aaaaccccgc cgaagcgggg ttttttgcga gatcctaatt atttaagctg
     3721 ggtaaatgca gataatcgtt ttctggcttc gcgatttgtc gcctgcatca ccatccacgg
     3781 actgaacgcc cacggcgtgg catcaatacc gtgtaataca tctgctaaat cacaccattg
     3841 ataatccatc acttcatcat cattgatctg taacgcacta gtggtgcgtg cggcaaatac
     3901 cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc gaaagtcagg
     3961 atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac ggcggatcac
     4021 tgcgtcttcg ttgctttctc ccagttgtgg gtgcccacaa accgagttag tccacacgcc
     4081 aggccatgct tttttgctca gtgcgcggcg ggtaactaat aattgtcctt tggcattaaa
     4141 cagccaactg gagaacgcga gatgtaagcg ggtgtctgcc gtgtgtgcgg catacttttc
     4201 cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg tttgcattat
     4261 cattacctcc tagatcctta ttcctttggt agaccagtct ttgcgtcaat caaagattcg
     4321 tttgtttctt gtgggcctga accgacttga gttaaaatca ctctggcaac atccttttgc
     4381 aactcaagat ccaattcacg tgcagtaaag ttagatgatt caaattgatg gttgaaagcc
     4441 tcaagctgct cagtagtaaa tttcttgtcc catccaggaa cagagccaaa caatttatag
     4501 ataaatgcaa agagtttcga ctcattttca gctaagtagt acaacacagc atttggacct
     4561 gcatcaaacg tgtatgcaac gattgtttct ccgtaaaact gattaatggt gtggcaccaa
     4621 ctgatgatac gcttggaagt gtcattcatg tagaatattg gagggaaaga gtccaaacat
     4681 gtggcatgga aagagttgga atccatcatt gtttcctttg caaaggtggc gaaatctttt
     4741 tcaacaatgg ctttacgcat gacttcaaat ctctttggta cgacatgttc aattctttct
     4801 ttaaatagtt cggaggttgc cacggtcaat tgcataccct gagtggaact cacatccttt
     4861 ttaatatcgc tgacaactag gacacaagct ttcatctgag gccagtcaga gctgtctgcg
     4921 atttgtactg ccatggaatc atgaccatct tcagcttttc ccatttccca ggccacgtat
     4981 ccgccaaaca acgatctaca agctgaacca gacccctttc ttgctattct agatatttct
     5041 gaagttgact gtggtaattg gtataactta gcaattgcag agaccaatgc agcaaagcca
     5101 gcagcggagg aagctaaacc agctgctgta ggaaagttat tttcggagac aatgtggagt
     5161 ttccattgag ataatgtggg caatgaggcg tccttcgatt ccatttcctt tcttaattgg
     5221 cgtaggtcgc gcagacaatt ttgagttctt tcattgtcga tgctgtgtgg ttctccattt
     5281 aaccacaaag tgtcgcgttc aaactcaggt gcagtagccg cagaggtcaa cgttctgagg
     5341 tcatcttgcg ataaagtcac tgatatggac gaattggtgg gcagattcaa cttcgtgtcc
     5401 cttttccccc aatacttaag ggttgcgatg ttgacgggtg cggtaacgga tgctgtgtaa
     5461 acggtcatct cataatcctc ctagatcctc acttatccag atacgtttcc gggtccttct
     5521 ccttgcgaac accccagtcg gcctgcgtaa catccagcca ttggactttg ctgaaacgtt
     5581 tgtcgttggc cgtttgtgcg cgcagatcga cgtcctgttt cgtgatgacg gcaatggcgt
     5641 cgtaaccacc cgcgcccgga atcaggcacg ttaacacgcc ctttaaggtt tgacagtcgt
     5701 ccagcagaga cgtctggacc ggtggttcga tatccgcgcc gctctccttc gtaattttgc
     5761 ggaagctacg gcgaatcgtt gccacggcat cgcggacctc cgtgatctcc gggtacttct
     5821 ggcaggtgca gtcattgcgc tccaggctct cgaagatttg gtcgctgtag tcgtcgtggg
     5881 tttcgtgcag gcggtccagc ttgctcagac catccatgaa acggctattg gcgtggtcca
     5941 gctccgtgta aatcttcagg ctttccggca tgtggctgtc gtaccagttt ttcactttct
     6001 gcaccagctt aaccgtttcg ctgccgttct tgatatcgcc catccataac gttaagccgc
     6061 tcggcaggtg attagactta atggtgatgt tccagtcctc ctcgtccacc agatgcgcca
     6121 gtttgctacc atacgtcgca gagccgatgt ccggcagatt gctgatcagg gccggcggaa
     6181 agcggcgata gcggatagag ccataggcgg ccgctgccac atcaaaacca gagccaatct
     6241 taccttgggc ctgacaatgt gccacctgcg ccaggttatg aatcacttcg cggtacttgt
     6301 ccacgttgtt ctccaggtcg ctcacgaaga agctcgctaa cgccgtcgtt aagacggtaa
     6361 ctaaaccggc gctagaaccc agacccgtct tcggcacctc ctcaatgcga tggctgtgga
     6421 agctcaggcg gcggttacca cgatgctccg taacagagtc ctcttggctg tggtacgcgt
     6481 cgtcgctgaa aatgtcgatg acgaacaggt tgcggttgca gtaatcgtcc atattcggct
     6541 tgaagtagct gaagacgttg gcaatgacct tctcgataaa tgggttcttg ctgccaccaa
     6601 tgctaaccgg gatgaagccg ctctttgggc taatgtggta cagccactcg ccgtccttga
     6661 actgcttgct cttcacacgc acttcgaact tgtcagagcc ctgcaggcta ccatacgggt
     6721 gggcaactgc atgcatacgg gcagataagc cgacgacgaa cgcctcgtac ttggtgtcta
     6781 acaccaggta gccaccggcc agcagtgcct tacctggcgc gctgaatgca cgtaattcgc
     6841 tcatatgatc tccctcctag atcctcaaga cgtccacggc agattggtgt tacccggcag
     6901 taacaggtcg tcgatctgct gcttcgtggt cgtcttgttt tcgaacagct ggaacaccag
     6961 gcttttgatc ttcaggtcct tgttcagatt tttggcgctc agcaggcaac agcccgtgcc
     7021 gcccaggtcc gtctcgaacg tctcgtagct gaagtcgtcc tgcagcttct tcttgaagct
     7081 gtcgatttgc tcctgcgtaa tgtcactgcg cagcagcgtt aaagagcagc caccgccacc
     7141 tgcacccgtt aatttcgtag agccaatgcg caggtcgtcg ctcaggtttt tgatcagttc
     7201 caggcccggg tgagacacac cgatagagac cagcaggccg tgattgatac ggatcagctc
     7261 cagcagctgc tcgtacagct cattgttggt ctccaccgcc tcatcgtcgg tgcccttgca
     7321 cttgctcagc ttggtcatga tctccagacc ctgcagcgcg cactcgccca tcgcgtccag
     7381 gatcggcttc atcacctccg ggaacttttc ggtgactaaa acacgcacgc gggcgactaa
     7441 atccttggtg ctgcgtggga tacgggtgta ggtcagaatc atcgggatcg ccgggaagtc
     7501 gtccaggaac ttgaagttgt tcgtgttgat cgtgccgttg tggctgtctt tctcgaacag
     7561 cagggcgttg ccatacgtgg caactgcgtt gtcaatgccg ctcggggtgc catgaatgca
     7621 cttctcgccg ataaaggccc actggttcac gatgtgcttg tcgttttcgc tcagtttctc
     7681 caggtcgttg ctgccaatca ggccacccag ataggccatc gccagcgcca ggctcacgct
     7741 gatagatgcg ctagagccta agcctgcgcc aatcggcagc gtgctcttca ggctgaactt
     7801 gatgttcttt gcgtgcgggc acaggcaaac gaacatgtac aggaagcaga acgcggcgtg
     7861 gtaatggaag ctctcgctta actgcgctaa cagcgggtcc agcagagaca ccagctcctg
     7921 agacagaccg tcggttgctt gctgggcttt ggccagcttt tggctgttca cctggtcctc
     7981 cgtgatcgcg ttgaagtcgt tgatgctcca cttgtggttg aagctgatgt ccggaaagtc
     8041 cagctcgatc gtgtccggtg cagaagattc gctgatcagc aggtaggtgc gtaacgcaga
     8101 cacagaggcg gcgacggccg gcttattgta caccgcagag tgctcgccga agatgatcac
     8161 cttacctggc gcagacgtca ggaatggcag agacatggtt atttcctcct agatcctgaa
     8221 attgttatcc gctcacaatt ccacacatta tacgagccgg atgattaatt gtcaacagct
     8281 catttcagaa tatttgccag aaccgttatg atgtcggcgc aaaaaacatt atccagaacg
     8341 ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac
     8401 agcttccgat ggctgcctga cgccagaagc attggtgcac cgtgcagtcg atgataagct
     8461 gtcaaaccag atccaattcg ctataaacgc agaaaggccc acccgaaggt gagccagtgt
     8521 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg
     8581 actgagcctt tcgttttatt tgatgcctgg agatccttaa aaagatccta attattgttg
     8641 tctaatttct tgtaaaatat gttccgctgc ttgtgtattt gcacggggtt cttttttcaa
     8701 agcttcagct actttagcaa tttcatcacc ttttgcccct acaacgatag ctaatgattt
     8761 atattgtaaa ctcatatgac cttgttgaat accttctgac acaagcgcgc gacatgctgc
     8821 aaagttttgc gctaaaccaa cggcagcaac tacatgacct aattcttgtg ctgactctac
     8881 atttagtagc tctaatgaag ctttagcaat tggtaatact tttgtaccac cgccaacgat
     8941 tgccaatgtc ataggcactt caattgtacc aatcaatcgt tgacgatctt gatcgtaacg
     9001 ccatgtagca ataccacgat actgtccgtc acgactcgcg tatgcatgcg cacttgcttc
     9061 tgcaccacgc gtatcatttc ctgttgctaa aacaacagca tgtatgccat tcataacacc
     9121 tttattatgt gttgctgcac gatgaatatc tacttgggcc aatacagaag cacgttccat
     9181 tcgtttggca acctcttctc cagttctctc gccccttgct aaatctttaa catcaatttc
     9241 gccttgaact ttaacaacgg acgctgttgc atgattggat aaaatactca ttaaaatgtc
     9301 gctttgcgga aattcatttt ttaaaaatgc agttatggcc tctaaaatcg tattaagcat
     9361 attagcgccc atagcatctt tcgtatcaac aaatactttt aaagatagta actgttgctc
     9421 aggaaatgta tcaatcgcta tacgttggta accaccacca cgcgctttaa tagaaggata
     9481 tgcctcatcc gcaattttat gaatttgctt ttctaaagct ttaatgtctg ctgataattt
     9541 ttcagtatcg tcaacgccat caaagacgat ttgacctatc ataatacgtt cagaagatac
     9601 cgttttaaat ccgccagtct gattcactag ctttgcacca taactagctg cagcgacaac
     9661 tgaaggctct tccaccatca taggtacaac atatgcctta tcgtccacaa tgatattcgg
     9721 taataatcca acgggtaatg caccttgcgc gatgacattt tcaattaaac tattggctac
     9781 ttcttcatcg attaatggat gattcagtaa aatgtcgaat tgttcttctg ataaccattg
     9841 cttatcaacc aattgttgta acttttcttt acgagataaa tgtcgaaaat tcttatctaa
     9901 actttgcatg gacatggtct gtttcctgaa tagatcctaa ttactctggt ctgtgatatt
     9961 cgcgaacgtt attttcaata tttgataagt agaaaatatg acgatcttca tgaacagcat
    10021 cttgttcttc gtcaaattca acgtcatcaa aacgtttgaa gaatgtttca tatgcatcaa
    10081 cagatacttc agtacggtta tttaataatg ctttatgtgc agcttgatct aaatgatctt
    10141 tgtagccttc aactaatgtc gcactataaa attcaccaac tgaacctgag ccataactga
    10201 ataaaccgat tgtttcacca gcttgtaaat ctcgattttc aagtaatgat attaggctta
    10261 aatataatga tccagtataa atattaccga cataacggtt ataatctaca gcatcttcat
    10321 atcctgaacg taaacgctct tgagttgttt catcagcgtt atcaatgatt gactctaatg
    10381 cctttttacc catttttgta aatggaacat ggaagcatag agatgcgaag tcagctagcg
    10441 acttaccttg acgttttgcg tattcattcc agctttgttg gaatgagcgg atataagcat
    10501 ctttagataa tgcaccatca actaatggat atttatgtcc agttggacgc cagaaatcat
    10561 aaacgtcttc agtgtaagca acagcatctt catttaatgc caaaatgctt ggattatgtg
    10621 caataaccat cgcaactgcg ccagcacctt gtgttggctc gccgcctgag ttcaatccat
    10681 aacgtgctgt atctgtagca ataactaata ctttttcatt cggtctagtt gctaaataat
    10741 ctttagctaa ttgaattgct ggtgttgcag cataacaagc ttctttcatt tcaaagcagc
    10801 gtgcaaaagg ttgaatacct aataagttgt gaatttgaac agcggctgct ttagcagcat
    10861 caactgctga ttcagttgcc acaattacca taccaatttt ctttttgtct tcgtctgtta
    10921 taatgtcctt agcagcgtta gcgcccattg aaacgatgtc ttggtttaca ggactaacag
    10981 ccatttcagt ttgaccaatt ccaattaaaa atttgtttgg gtctacttgg cgtgcttctg
    11041 ctaatttagc catgtctaca tagtactttg gaacgtaaaa gtttatttta tcgataccta
    11101 ttgtcataag gtttctcctg aatagatcct caattcaacc gttcaatcac catcgcaatt
    11161 ccctgaccgc cgccaatgca cagtgttgcc agccccagcg ttttatcgcg tgcctgcatg
    11221 gcatgtaata gtgtgaccag aatacgagca ccactggcac cgataggatg cccgagcgcg
    11281 atggccccgc cgttgacatt cactttctca gaatcaaagc ccaggttttt cccaacggca
    11341 aggaactgtg cagcaaatgc ttcattagcc tcaatgagat caatatccgc cagttgcagc
    11401 cccgccagtt gtaacgcttt ttgcgtggca ggtactggcc ccatacccat caatgcgggg
    11461 ggcacgccac cgctggcata acttttaatg cgagccaggg gggtaaggcc tgctgccagc
    11521 gccgcagatt cttccataat caccagagcg gcagcaccgt cgttaatacc agacgcgttc
    11581 ccagcggtga ctgttcctgc tttatcgaag gccgggcgca atgcacctaa cgcttcagcc
    11641 gttgagttcg ctttcgggaa ctcgtcttga ctgaagacga aggttttctt tcgagtgaca
    11701 acatttaccg ggacgatttc ggctgtaaaa gcaccggact caattgcggc tgccgcttta
    11761 cgctgtgaat gtagcgccag ttcatcctgc atttcacggg taattccgta ctctttagcc
    11821 acgttttcgg cggtaatccc catatgataa ccatgggtgg cgcacatcag gccatcgcgc
    11881 aggattacgt cataaacctg tccgtctcca agacgataac cagagcgtgc ttttgcatcg
    11941 agtaagtagg gggctaaact catattttcc ataccccccg ccacaatgct ctgcgcctga
    12001 cctgcctgaa tggcctgggc ggcaagcgcc acacttttaa gacccgaacc acatacttta
    12061 ttgaccgtga atccgcacac cgtttctgcc agcccgcttt ttaacagtgc ctgacgcgcc
    12121 ggattttgcc ccagcccggc ttgtaacacg ttacccataa tcacttcatc aacgtgttgt
    12181 gaatcgattt ttgcacgttc aatggcggct ttaattactg tcgcccccag gtcgatggcg
    12241 ctggtggaag cgagtgaacc gttaaaacta ccgatagcag tacgtaccgc actgacgatg
    12301 acacaatttt tcattttata ttcctcctaa gatcttttga attctgaaat tgttatccgc
    12361 tcacaattcc acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaaa
    12421 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
    12481 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
    12541 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
    12601 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
    12661 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc
    12721 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
    12781 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
    12841 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
    12901 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
    12961 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
    13021 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
    13081 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
    13141 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
    13201 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
    13261 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
    13321 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
    13381 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
    13441 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
    13501 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
    13561 attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata cgacgatacc
    13621 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
    13681 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
    13741 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
    13801 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    13861 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccgac
    13921 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    13981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    14041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    14101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    14161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    14221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    14281 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    14341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    14401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    14461 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    14521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    14581 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    14641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    14701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    14761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    14821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    14881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt acgagctcgc
    14941 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
    15001 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagt aacaacttat
    15061 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga
    15121 aatattttat ctgattaata agatgatctt c
//
