LOCUS       sequence-496834-       10879 bp    DNA     circular SYN 09-JAN-2026
DEFINITION  Retroviral vector encoding C-terminally FLAG tagged mouse GCN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S
  TITLE     Integrated stress response of vertebrates is regulated by four 
            eIF2alpha kinases.
  JOURNAL   Sci Rep. 2016 Sep 16;6:32886. doi: 10.1038/srep32886.
  PUBMED    27633668
REFERENCE   2  (bases 1 to 10879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 9, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-16"; volume: "6:32886. doi"; pages: " 
            10.1038/srep32886"
COMMENT     Sequence Label: pMXs-mGCN2-FLAG-IP
FEATURES             Location/Qualifiers
     source          1..10879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5051..5116
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(5158..5732,5733..5735,5736..5744)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5325..5342)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5552..5571
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5745..6347
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MVTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6244..6264
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6518..6987
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7117..7134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7288..7876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7368..7387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8040..8831,8832..8900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8663..8682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8901..9005)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             9035..9628
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9691..10048
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             10107..10523
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10453..10475
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10491..10507
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(10533..10879,1..28)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     10845..10861
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa ggatcccagt
       61 gtggtggtac gggaattcta gagccaccat ggctgggggc cgcggggcct ctggacgcgg
      121 ccgggctgag ccacaggaga gctattcgca gcgacaggac cacgagctgc aggccctgga
      181 ggccatttac ggctcggact tccaggatct gcggccggat gcgcgcggac gggtgagaga
      241 gcctcctgaa atcaacttag ttctttaccc tcagggccta gctggtgaag aagtatacgt
      301 gcaagtggaa ctgcaggtta aatgcccgcc aacataccca gatgtagttc ccgaaataga
      361 gttaaaaaat gctaaaggcc tgtcgaatga aagtgttaat ttgttaaaat cacacctgga
      421 agaactggcc aaaaagcagt gtggagaggt gatgatattt gaactggcac accatgtgca
      481 gtcgtttctc agcgagcata acaagccccc accaaagtct ttccatgaag aaatgctgga
      541 aaggcaggct caggagaagc agcagaggtt gctggaggcc aggcggaagg aggagcagga
      601 gcaacgtgaa atcctccacg agattcagag aaggaaagag gagatcaagg aagagaagaa
      661 aaggaaggaa atggctaagc aggaacgttt ggaaatcact agcttgacaa accaggacta
      721 tgcctctaag agagacccag cagggcacag ggcagctgcc atcctccatg gaggctctcc
      781 tgactttgta ggaaatggta aagcccggac atactcctca ggaaggtcca ggcgagaacg
      841 tcagtattct gtgtgtagtg gtgaaccctc tcctggctcg tgtgacatcc tccatttctc
      901 tgtgggtagc cctgatcagc tcatggtgca caaagggaga tgtgttggca gtgatgagca
      961 gctgggaaag gtggtataca atgctttgga aacagccacg ggcagctttg tcttgctgca
     1021 tgagtgggtc cttcagtggc agaagatggg cccttgcctt accagtcaag agaaagagaa
     1081 gattgacaag tgcaaaaggc agattcaagg agcagaaaca gaattcagct ccctagtgaa
     1141 actgagccat ccaaatatcg tccgctactt tgcaatgaac tccagagagg aggaggactc
     1201 cattgtgatc gacattctgg cagagcacgt cagtggcatc tctctggcta cacacctgag
     1261 ccactcgggc ccagtccccg cccaccagct gcgcaagtac acagcccagc tcctggcagg
     1321 cctggattac ctacacagca actccgtggt gcacaaggtt ctgagcgcgt ccagtgtctt
     1381 ggtagatgcc gagggcactg tcaagataac agactacagc atctctaagc gtctggcaga
     1441 catttgcaag gaggatgtat ttgagcaagc tcgagttcgt tttagtgaca gtgccctgcc
     1501 ttataaaaca ggaaagaaag gggatgtgtg gcgtcttggc ctcctgctgt tgtctctcag
     1561 ccaaggacag gagtgtgggg agtatccggt gaccatcccc agtgacctgc cagctgactt
     1621 ccaagacttc ctgaagaagt gtgtctgcct ggacgacaag gaaagatgga gccctcagca
     1681 gctgctgaaa cacagcttta taaaccctca accaaagctg cctttggtgg agcagagtcc
     1741 tgaagactct gggggacaag actacattga gaccgtcatt cccagcaacc agctgcccag
     1801 cgctgcgttc ttcagtgaga cacagaaaca gttctcccgg tacttcattg agtttgaaga
     1861 actacaactc ctagggaaag gagcttttgg agctgtcatc aaggtgcaaa acaagcttga
     1921 tggctgctgc tatgctgtga agcgcatccc tatcaaccct gcaagcagac acttccgccg
     1981 tatcaagggc gaggtgacac tgctatcgcg cctgcaccat gagaacattg tgcgctacta
     2041 caacgcctgg atcgagcggc atgagcgccc agcggtgcca gggacaccgc ccccagactg
     2101 cacaccccag gcccaggaca gcccagccac ctacgggaaa acatcaggcg acactgaaga
     2161 actgggcagc gtggaggctg cagcaccgcc acccatcctc agcagctcgg tggagtggag
     2221 cacatctgca gagcgttcta ccagcacccg cttcccagtc acgggccagg attccagcag
     2281 cgatgaggag gacgaggacg agcgcgatgg cgtcttctcc cagtcctttc tacctgcttc
     2341 agattctgac agtgacatca tctttgacaa tgaagatgaa aacagtaaaa gtcaaaatca
     2401 ggatgaagac tgcaatcaaa aggacggtag ccatgaggtt gagccttcag tgacagctga
     2461 agctgtgcac tacctttata tccagatgga gtactgcgag aagagcacgc tgcgggacac
     2521 cattgaccag gggctgttcc gagacaccag ccggctctgg aggcttttcc gagagattct
     2581 ggatggatta gcttatatcc atgagaaagg aatgattcat cgcgacttga agcctgtcaa
     2641 tatttttttg gattctgatg accatgtgaa aataggtgac tttggcctgg caacagacca
     2701 tctggccttc actgctgaag gtaaacagga cgaccaggca ggtgatcgag tgattaagtc
     2761 agacccttca ggccatttga ctggcatggt tggtactgct ctgtatgtaa gccctgaggt
     2821 ccaaggaagc accaagtctg catacaacca gaaagtggat ctcttcagcc tgggaattat
     2881 cttctttgag atgtcctatc acccgatggt gactgcctca gaaagaattt tcgttctcaa
     2941 ccaacttcga gatcccacat cgcccaagtt tccagacgac ttcgatgatg gagaacatac
     3001 aaagcagaaa tctgtcatct cctggctgtt gaaccatgat ccagctaaac ggcccacggc
     3061 catggaactg ctcaagagtg agctgctccc cccaccgcag atggaggaat ccgagctgca
     3121 tgaagtgctg caccacacgc tggccaacat agatgggaag gcctaccgca ccatgatgag
     3181 tcagatcttc tgccagcaca tctcccccgc tatagactac acctacgaca gtgacatcct
     3241 gaagggcaac ttcttgattc gtacagccaa gatccagcag cttgtatgtg aaaccatcgt
     3301 ccgcgtcttt aaaaggcatg gcgctgtcca gctgtgcacc ccactgctgc ttccccgaaa
     3361 caggcaaata tatgagcaca acgaagcggc tttgttcatg gatcacagcg gcatgctggt
     3421 gatgcttccg ttcgacctgc gggtcccttt tgcaagatat gtggcaagaa ataacatact
     3481 gaatttaaaa cgatactgca tagagcgggt gttcagacct cggaagttag accgatttca
     3541 tcccaaagaa ctcctggaat gtgcatttga tatcgtcact tctaccacca acagctctct
     3601 gcccactgcc gaaaccatct acaccatcta tgaaataatc caggagtttc ctgcacttca
     3661 ggaaaggaat tacagcattt acttgaacca tacgatgctt ctgaaggcaa tactcctaca
     3721 ctgtgggatc ccggaggaca agctcagcca agtctacgtc attctgtatg atgctgtgac
     3781 agagaagctg actaggagag aagtggaagc taaattctgt aacctgtcct tgtcttctaa
     3841 tagcctgtgt agactctaca agttcatcga gcagaagggg gatttgcaag acttaacgcc
     3901 aaccatcaac tcactaataa aacagaaaac aggcgttgct cagttggtga agtatagctt
     3961 aaaagacctc gaggacgtcg tcgggctgct gaagaaactt ggtgtgaagt tacaggtctc
     4021 catcaacctg ggcttggtct acaaggtgca gcagcacact ggcatcatct tccagttcct
     4081 ggcattcagc aaacgcaggc agagggttgt gcctgagatc cttgccgctg gtggcagata
     4141 cgacctgctg attcccaagt tcagaggccc acagactgtg gggccagtcc ccactgctgt
     4201 cggtgtcagc atagccatag acaagatatt tgctgttgtc ctcaacatgg aagagcctgt
     4261 tacagtgagc tcctgtgacc tcctggttgt cagtgttggc cagatgtcca tgtccagggc
     4321 catcaaccta acccagaaac tgtggacagc gggcatcact gcagaaatca tgtatgactg
     4381 gtcccagtcc caggaagagt tacaagagta ctgcagacac catgaaatca cctatgtggc
     4441 tctggtctcc gataaagaag gaagccatgt caaggtcaag tcctttgaga aggagaggca
     4501 aacagaaaag cgtgtattgg aatcggatct tgtggatcac gttatgcaga aactaagaac
     4561 caaagttggt gacgaaagaa atttcagaga cgcttctgat aatcttgccg tacaaactct
     4621 gaaggggtcg ttttctaatg cttcaggttt gtttgaaatc catggaacta cagtagtccc
     4681 caacgtgatt gttctagcac cagaaaagct gtcagccagc accaggaggc gacatgagat
     4741 tcaggtgcaa acccgacttc agactacact tgccaactta catcagaaaa gcagtgaaat
     4801 tgaaattttg gctgtggacc tacccaagga aacaatctta cagtttctat cattagagtg
     4861 ggatgctgat gaacaggcat ttaacacaac tgtgaagcag ctgctgtcac gcctgccaaa
     4921 gcaaagatac ctcaaactcg tctgcgatga aatttataac atcaaagttg aaaagaaggt
     4981 gtcagtgctg ttcctgtaca gctacagaga tgactactac agaatcctat ttgtccccga
     5041 ggccaaggcc gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5101 ggatgacgat gacaagtagt gactgcggcc gccgctacgt aaattccgcc cccccccccc
     5161 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     5221 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     5281 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     5341 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     5401 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     5461 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     5521 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     5581 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     5641 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     5701 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtt accgagtaca
     5761 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5821 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5881 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5941 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     6001 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     6061 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     6121 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     6181 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     6241 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     6301 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtc gacgataaaa
     6361 taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt
     6421 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
     6481 gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
     6541 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
     6601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6661 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
     6721 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     6781 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     6841 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
     6901 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
     6961 tgactacccg tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt
     7021 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     7081 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7141 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7921 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7981 ccttttgcgg ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8881 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8941 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9001 ccgcgcacat ttccccgaaa agtgccacct gcataatgaa agaccccacc tgtaggtttg
     9061 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     9121 aaaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     9181 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     9241 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     9301 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     9361 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     9421 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     9481 ggcgcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     9541 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     9601 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
     9661 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
     9721 cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta
     9781 gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc
     9841 tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag tccaaaaatc
     9901 ccgatcgttt tggactcttt ggtgcacccc cctaatagga gggatatgtg gttctggtag
     9961 gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc
    10021 gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg
    10081 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
    10141 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
    10201 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
    10261 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
    10321 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
    10381 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
    10441 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
    10501 ccctcactcc ttctctaggc gcccccatat ggccatatga gatcttatat ggggcacccc
    10561 cgccccttgt aaacttccct gaccctgaca tgacaagagt tactaacagc ccctctctcc
    10621 aagctcactt acaggctctc tacttagtcc agcacgaagt ctggagacct ctggcggcag
    10681 cctaccaaga acaactggac cgaccggtgg tacctcaccc ttaccgagtc ggcgacacag
    10741 tgtgggtccg ccgacaccag actaagaacc tagaacctcg ctggaaagga ccttacacag
    10801 tcctgctgac cacccccacc gccctcaaag tagacggcat cgcagcttgg atacacgccg
    10861 cccacgtgaa ggctgccga
//
