LOCUS       sequence-496941-       11592 bp    DNA     circular SYN 12-JAN-2026
DEFINITION  Dual-luciferase GLI reporter viral plasmid. 8xGLI binding site 
            followed by minPro-luc2P expression, in addition to constitutive 
            SV-40-driven Renilla luciferase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Krantz S, Bell B, Lund K, Parra NS, Ng Y, De Oliveira Rosa N, 
            Mukhopadhyay S, Croix BS, Sarin KY, Weigert R, Raimondi F, 
            Iglesias-Bartolome R
  TITLE     Dissection of Galphas and Hedgehog signaling crosstalk reveals 
            therapeutic opportunities to target adenosine receptor 2b in 
            Hedgehog-dependent tumors.
  JOURNAL   bioRxiv [Preprint]. 2025 Feb 27:2025.02.21.639530. doi: 
            10.1101/2025.02.21.639530.
  PUBMED    40060632
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Feb 27:2025.02.21.639530. doi: 
            10.1101/2025.02.21.639530."
COMMENT     Sequence Label: pLenti X1 Puro 8xGLI_minPro_Luc2p_Ren
FEATURES             Location/Qualifiers
     source          1..11592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(149..217,218..1009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(367..386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1180..1768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1669..1688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1922..1939
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2056..2077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2092..2097,2098..2115,2116..2122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2130..2146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2135..2157
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2154..2170
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2154..2170
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2189..2209
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2191..2209
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2237..2463
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2464..2644
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2691..2816
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3309..3542
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3727..3771
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4038..4154
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4188..4212
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4277..4325
                     /note="synthetic polyadenylation signal"
     misc_feature    4339..4430
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4379..4398
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        4804..4835
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      4862..4871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4868..6517
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             6521..6640
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    6692..6813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7007..7364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7007..7198
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7030..7050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7215..7350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8354..8402
                     /note="synthetic polyadenylation signal"
     protein_bind    complement(8433..8457)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8488..9076
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8541..8561)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8959..8970)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9090..9589
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9125..9148)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9522..9541
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9610..10209
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9610..9629)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10106..10126
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10347..10580
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10652..10773
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10813..10948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10968..10987)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10969..10987)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10997..11014)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10997..11013)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11006..11028)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11155..11583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11242..11261)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11452..11473
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
       61 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1801 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1921 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     1981 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2041 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2101 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2161 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     2221 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     2281 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     2341 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     2401 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     2461 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2521 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2581 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2641 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2701 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     2761 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     2821 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     2881 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     2941 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3001 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3061 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3121 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     3181 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     3241 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     3301 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     3361 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     3421 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     3481 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     3541 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     3601 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     3661 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     3721 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     3781 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     3841 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     3901 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     3961 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     4021 cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     4081 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     4141 aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     4201 aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccga attcgcggcc
     4261 gcattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4321 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4381 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct ggcctaactg
     4441 gccggtacac gcgtagattc gcgatcgacc tcgacaagca gggaacaccc aagtagaagt
     4501 tccgacaagc agggaacacc caagtagaag ctctcgacct cgacaagcag ggaacaccca
     4561 agtagaagct ccgacaagca gggaacaccc aagtagaagc tctcgacctc gacaagcagg
     4621 gaacacccaa gtagaagctc cgacaagcag ggaacaccca agtagaagct ctcgacctcg
     4681 acaagcaggg aacacccaag tagaagctcc gacaagcagg gaacacccaa gtagaagctc
     4741 tcgatcgcga atctgtggta ccgaggatat caagatctgg cctcggcggc caagcttaga
     4801 cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa
     4861 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga
     4921 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg
     4981 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga
     5041 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat
     5101 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat
     5161 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat
     5221 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa
     5281 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta
     5341 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga
     5401 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag
     5461 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg
     5521 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct
     5581 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg
     5641 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca
     5701 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag
     5761 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc
     5821 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca
     5881 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa
     5941 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac
     6001 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat
     6061 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct
     6121 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct
     6181 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct
     6241 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg
     6301 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat
     6361 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt
     6421 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat
     6481 tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga
     6541 ggtggaggag caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga
     6601 tagacaccct gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagagt
     6661 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     6721 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     6781 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     6841 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     6901 ggtaaaatcg ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac
     6961 catggcctga aataacctct gaaagaggaa cttggttagc taccttctga ggcggaaaga
     7021 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc gattcttctg
     7381 acactagcgc caccatgacc agcaaggtgt acgaccccga gcagaggaag aggatgatca
     7441 ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact
     7501 actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccgcta
     7561 gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc
     7621 ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac aggctgctgg
     7681 accactacaa gtacctgacc gcctggttcg agctcctgaa cctgcccaag aagatcatct
     7741 tcgtgggcca cgactggggc gcctgcctgg ccttccacta cagctacgag caccaggaca
     7801 agatcaaggc catcgtgcac gccgagagcg tggtggacgt gatcgagagc tgggacgagt
     7861 ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag aagatggtgc
     7921 tggagaacaa cttcttcgtg gagaccatgc tgcccagcaa gatcatgaga aagctggagc
     7981 ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg agaagaccca
     8041 ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac gtggtgcaga
     8101 tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag atgttcatcg
     8161 agagcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca
     8221 ccgagttcgt gaaggtgaag ggcctgcact tcagccagga ggacgccccc gacgagatgg
     8281 gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagtaa actctagaac
     8341 cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     8401 tgttcgaact agatgctgct cgagatatct agacccagct ttcttgtaca aagtggttga
     8461 tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga
     8521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8881 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9001 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9061 tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     9121 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     9181 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     9241 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     9301 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     9361 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     9421 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     9481 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     9541 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     9601 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     9661 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     9721 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     9781 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     9841 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     9901 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     9961 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
    10021 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
    10081 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
    10141 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
    10201 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
    10261 gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    10321 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
    10381 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10501 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10561 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
    10621 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
    10681 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10741 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
    10801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10921 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
    10981 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    11041 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    11101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
    11161 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    11221 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    11281 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    11341 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    11401 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    11461 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    11521 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    11581 attttaacaa aa
//
