LOCUS       sequence-496954-        3395 bp    DNA     circular SYN 12-JAN-2026
DEFINITION  to generate mRNA for membrane mGold2t for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Tschanz A, Matus DQ, Swinburne I
  TITLE     Systematic comparison of mStayGold and mGold2 variants for live 
            imaging in zebrafish.
  JOURNAL   MicroPubl Biol. 2025. 10.17912/micropub.biology.001847.
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol. 2025. 10.17912/micropub.biology.001847."
COMMENT     Sequence Label: pDQM139
FEATURES             Location/Qualifiers
     source          1..3395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     816..837
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    957..1078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(968..987)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1022..1041
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1231..1247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1231..1247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1244..1266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1419..2007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1499..1518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2124..2148)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2248..3039,3040..3108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2871..2890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3109..3213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3312..3334
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3326..3343
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3327..3343
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atttaggtga cactatagac tagtgccacc atgggctgca tcaagagcaa gcgtaaggac
       61 aacctgaacg atgatgaagc tgctatggga tgtatcaaat ctaagcgtaa ggataacctt
      121 aatgatgatg agggagcagc tgctcccgct gctgctggat ccatggcgag caagggcgaa
      181 gagctgttca ccggagtcgt ccccatcctg gtcgaactgg atggagacat caacggtcac
      241 aagttcagcg tctccggcga gggcgagggt gatgctacct acggcaagct gaccctgaag
      301 ttcatctgca ccaccggcaa gctgcctgtc ccctggccca ccctcgtgac cagcctgggc
      361 tacggcctgc agtgcttcgc tcgctaccct gataacatga agcgtcacga tttcttcaag
      421 tccgctatgc ccgaaggata cgtccaggag cgtaccatct tcttcgagga tgacggcaac
      481 tacaagaccc gcgctgaagt caagtttgaa ggcggaaccc tggtgaatcg cgtcgaactg
      541 aagggaatcg acttcaagga ggacggcaac atcctggggc acaagctgga atacaactac
      601 aactgccaca acgtctttat caccgccgat agacagcaaa acggcatcaa agtcaacttc
      661 aagatccgtc acaacatcga agtcggaggc gtgcagctcg ccgaccacta ccagcagaac
      721 acccccattg gcgatggacc cgtcctgctg cccgacaacc actacctgag ctaccaaacc
      781 aagctgtcca aagatcccaa tgagaaacgc gatcacatgg tcctgctgga gttcgtgacc
      841 gccgccggaa tcactctctc aatggatgag ctgtacaagt aagaattcat acgtatccgg
      901 aaccggtgct agcttgactg actgagatac agcgtacctt cagctcacag acatgataag
      961 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1021 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1081 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1141 aagcaagtaa aacctctaca aatgtggtat tggcaggcta ggtggaggct cagtgatgat
     1201 aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta
     1261 tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg
     1321 agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa
     1381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2041 gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa
     2101 tcgtcctcgt gataccaatt cggagcctgc ttttttgtac aaacttgttg ataatggcaa
     2161 ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag agccacgctc
     2401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2461 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2521 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2581 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2641 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2701 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2761 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2821 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2881 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2941 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3001 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3061 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3121 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3181 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccagatac
     3241 ctgaaacaaa acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc
     3301 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg
     3361 catctggaat aaggaagtgc cattccgcct gacct
//
