LOCUS       sequence-496977-       14166 bp    DNA     circular SYN 12-JAN-2026
DEFINITION  Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into 
            TRV2 with mutation SlFT element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Liu Y, Weiss T, Lee J, Powell J, Choo SYC, Roshannai E, Kamalu M, 
            Amerasekera J, Feng S, Jacobsen SE
  TITLE     Virus induced transgene- and tissue-culture free heritable genome 
            editing in tomato
  JOURNAL   bioRxiv 2025.11.08.687402
REFERENCE   2  (bases 1 to 14166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.11.08.687402"
COMMENT     Sequence Label: pYL001
FEATURES             Location/Qualifiers
     source          1..14166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2349..2396
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             2403..2423
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    3746..3870
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3895..3900,3901..3918,3919..3925)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3979..4659
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             4979..5284
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5163..5182
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5325..5449)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           5461..5528
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             6096..6113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6148..6400
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6422..6446
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7747..8376
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8805..9878
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9944..10138
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10374..10396)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10482..10622
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10537..10556
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10637..10654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10808..11396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10888..10907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12821..13615)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14040..14064
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gcacacaagg ttaaaaacgc tgtagtaata catgcgcaag aacaggctga
     2161 gcatcttgtt ctggggtttc acactatctt tagagaaagt gttaagttaa ttaagttatc
     2221 ttaattaaga gcataattat actgatttgt ctctcgttga tagagtctat cattctgtta
     2281 ttaaaaattt gacaactcgg tttgctgacc tactggtcac tgtatcactt acccgagtta
     2341 acgagatgga ttacaaggat gatgatgata aggattacaa ggatgatgat gataagatgg
     2401 ctccaaagaa gaagagaaag gttggaatcc acggagttcc agctgctatg ctgcagcata
     2461 aagcctatga ataccgtatc tatccagata agaagcagga aacactgatt gccaagacaa
     2521 taggcagttc aaggtatgta tacaaccatt tcctggaact ttggaacaag gaatatgagg
     2581 agaccggaaa aggtctgact tattacgcat gctccaaact tctgacaaag cttaagaggg
     2641 acccggaaac agtatggctt tgtgaagtgg ataagttctc gctccagaat tcgcttcgta
     2701 acctgtcgga cgccttctcc cgctttttca aaggacagaa cgagcatccc cagttcaaga
     2761 gcaagaagag cccgaggcaa agctacacga cacaatatac gaacaacaac atcgcagttt
     2821 ccggaaattg tctgaagctg cctaagctgg gccttgtcaa gtttgcagac agtagggaga
     2881 tgaaaggccg tatactgaat gccacagtac gaaggaaatc gagcgggaaa ttcttcgtat
     2941 cgatcctatg cgaagaggag atctgtgaat tgccaaagac tgactcatct gtcggaattg
     3001 acctcggaat cattgacttt gcggttatgt cagacggaag caggcatgac aacaatcatt
     3061 ttaccaggca aatggaagaa aggcttagac gggagcagcg aaagcttgca aggcgtgcac
     3121 ttgctgcgga gaaaaggggc atttcccttt ctgaagccag gaactatcag aagcaaaggc
     3181 ggaaggtagc gagactttat gaaaaggttg caaaccagcg aaaagaatat cttaacaaac
     3241 tcagtacaga gatagtcaaa aaccacgata tcatctgtat cgaggacctt aacgttaagg
     3301 gcatgatgcg caatcataaa ctggcaaaaa gcatctctga tgtatcatgg acgagccttg
     3361 tatcgaaact gcagtacaag gcttcctggt atggaaaaga agtcatcagg ataagcagat
     3421 ggtttccgtc aagtcagata tgctcagagt gcggccacaa ggacaggaag aagcctctcc
     3481 atgtaaggga gtggacctgt cctgtttgtc atgcccacca tgaccgtgac gtcaatgcag
     3541 caagaaatat actggctgag ggactcagga taagagctct gactccgggg tcttagcaat
     3601 acagaagcaa acaggaaccg caggaattgc gggggtagct tggtaaacaa gagaaacctc
     3661 tgccggcaaa gaaataagcc ggtaagtatg ctctgttccc aagaatctcg tgactttagt
     3721 catgagagtt tcaacatcgc aggtgacaag tttgtacaaa aaagctgaac gagaaacgta
     3781 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     3841 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggaacccc aggctttaca
     3901 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     3961 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     4021 caatggcatc gtaaagaaca ttttgaggca tttcagtcag tcgctcaatg tacctataac
     4081 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     4141 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     4201 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     4261 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     4321 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     4381 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     4441 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     4501 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     4561 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     4621 tggcaggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt
     4681 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     4741 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     4801 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     4861 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     4921 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     4981 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     5041 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     5101 gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     5161 gcgcatgatg accaccgata tggccagtgt gccggcctcc gttatcgggg aagaagtggc
     5221 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     5281 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     5341 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     5401 tatttatatc attttacgtt tctcgttcag cttttttgta caaacttgtc acctgcgatg
     5461 ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt cggcatggcg
     5521 aatgggacta gcctagagaa cgtgatcctc ttgttgttgg tcgtgtggta ggggatgtat
     5581 tggacccttt cacaagaact attggcctaa gagttatata tagagataga gaacccgggc
     5641 atgtcccgaa gacattaaac tacggttctt taagtagatc cgtgtctgaa gttttaggtt
     5701 caatttaaac ctacgagatt gacattctcg actgatcttg attgatcggt aagtcttttg
     5761 taatttaatt ttctttttga ttttatttta aattgttatc tgtttctgtg tatagactgt
     5821 ttgagatcgg cgtttggccg actcattgtc ttaccatagg ggaacggact ttgtttgtgt
     5881 tgttatttta tttgtatttt attaaaattc tcaacgatct gaaaaagcct cgcggctaag
     5941 agattgttgg ggggtgagta agtactttta aagtgatgat ggttacaaag gcaaaagggg
     6001 taaaacccct cgcctacgta agcgttatta cgcccgtctg tacttatatc agtacactga
     6061 cgagtcccta aaggacgaaa cgggagaacg ctagccacca ccaccaccac cacgtgtgaa
     6121 ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     6181 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     6241 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     6301 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     6361 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa ctatcagtgt
     6421 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     6481 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     6541 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     6601 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     6661 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     6721 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     6781 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     6841 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     6901 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     6961 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     7021 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     7081 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     7141 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     7201 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     7261 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     7321 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     7381 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     7441 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     7501 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     7561 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     7621 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     7681 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     7741 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     7801 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     7861 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     7921 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     7981 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     8041 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     8101 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     8161 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     8221 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     8281 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     8341 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     8401 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     8461 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     8521 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     8581 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     8641 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     8701 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     8761 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     8821 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     8881 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     8941 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     9001 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     9061 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     9121 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     9181 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     9241 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     9301 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     9361 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     9421 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     9481 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     9541 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     9601 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     9661 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     9721 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     9781 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     9841 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     9901 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     9961 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    10021 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    10081 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    10141 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    10201 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    10261 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    10321 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    10381 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    10441 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    10501 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    10561 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    10621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11221 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    11281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaagggc
    11461 tgatgaatcc cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt
    11521 catatgatca aatggtttcg ccaaaaatca ataatcagac aacaaaatgt gcgaactcga
    11581 tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag
    11641 cttaacgttg gcttgccacg ccttacttga ctgtaaaact ctcactctta ccgaacttgg
    11701 ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt
    11761 aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat
    11821 ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatatcc gtgagcaaaa
    11881 acggcttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga
    11941 gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga
    12001 ccttgcgagc attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa
    12061 agtgccatgg tataaatccg ttgagaagct gggttggtac tggttaagtc gagtaagagg
    12121 aaaagtacaa tatgcagacc taggagcgga aaactggaaa cctatcagca acttacatga
    12181 tatgtcatct agtcactcaa agactttagg ctataagagg ctgactaaaa gcaatccaat
    12241 ctcatgccaa attctattgt ataaatctcg ctctaaaggc cgaaaaaatc agcgctcgac
    12301 acggactcat tgtcaccacc cgtcacctaa aatctactca gcgtcggcaa aggagccatg
    12361 gattctagca actaacttac ctgttgaaat tcgaacaccc aaacaacttg ttaatatcta
    12421 ttcgaagcga atgcagattg aagaaacctt ccgagacttg aaaagtcctg cctacggact
    12481 aggcctacgc catagccgaa cgagcagctc agagcgtttt gatatcatgc tgctaatcgc
    12541 cctgatgctt caactaacat gttggcttgc gggcgttcat gctcagaaac aaggttggga
    12601 caagcacttc caggctaaca cagtcagaaa tcgaaacgta ctctcaacag ttcgcttagg
    12661 catggaagtt ttgcggcatt ctggctacac aataacaagg gaagactcac tcgtggctgc
    12721 aaccctgctt actcaaaatc tattcacaca tggttacgtt ttggggaaat tatgagggga
    12781 tctctcagcg ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta
    12841 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
    12901 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
    12961 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
    13021 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
    13081 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
    13141 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
    13201 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
    13261 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
    13321 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
    13381 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
    13441 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
    13501 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
    13561 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt
    13621 atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac
    13681 gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc
    13741 aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg
    13801 gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
    13861 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
    13921 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
    13981 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
    14041 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
    14101 acgtttttaa tgtactgaat taacgccgaa ttaattccta ggccaccatg ttgggcccgg
    14161 cgcgcc
//
