LOCUS       sequence-496979-        3518 bp    DNA     circular SYN 12-JAN-2026
DEFINITION  HyPB RFP cargo plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ivancic D, Agudelo A, Lindstrom-Vautrin J, Jaraba-Wallace J, Gallo 
            M, Das R, Ragel A, Herrero-Vicente J, Higueras I, Billeci F, 
            Sanvicente-Garcia M, Petazzi P, Ferruz N, Sanchez-Mejias A, Guell M
  TITLE     Discovery and protein language model-guided design of hyperactive 
            transposases.
  JOURNAL   Nat Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4.
  PUBMED    41039042
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4."
COMMENT     Sequence Label: pTIR_HyPB_RFP
FEATURES             Location/Qualifiers
     source          1..3518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      573..1161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1062..1081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1212..1274
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1311..1522
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1535..1803
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1831..1840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2576..2596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    2638..2759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2675..2694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2729..2748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   2826..2860
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2869..2887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2955..3059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3060..3128,3129..3518,1..402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3278..3297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
       61 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      421 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgctgcctta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt
     1261 aaaattgacg catgtatgaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     1321 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     1381 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     1441 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     1501 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     1561 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     1621 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     1681 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     1741 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     1801 atccaagctg tgaccggcgc ctacgatatc gccaccatgg ccagctccga ggatgtcatc
     1861 aaagagttta tgagatttaa ggtcaagatg gagggaagcg tcaacggaca cgagttcgag
     1921 attgagggag aaggagaagg ccggccttac gagggcacac aaaccgctaa gctcaaggtc
     1981 acaaaaggag gacccctccc cttctcctgg gatattctga gccctcagtt ccagtacgga
     2041 agcaaagcct atgttaaaca ccctgccgac atccctgact atctgaagct ctccttccct
     2101 gaaggcttca agtgggagag attcatgaac ttcgaggacg gaggcgtggt gacagtcaca
     2161 caagatagca ccctccagga cggagagttt atttataagg tgaaactcag aggaaccaac
     2221 ttcccctccg atggccctgt catgcaaaaa aaaacaatgg gatgggaagc ctccaccgag
     2281 agaatgtatc ctgaggatgg cgctctgaaa ggcgaaatta aaatgagact gaaactcaaa
     2341 gacggaggac actacgatgc cgaggtcaaa acaacctaca aggccaagaa acaagtgcag
     2401 ctgcctggcg cctacatgac tgatattaaa ctcgacatta tcagccataa tggggactac
     2461 accatcgtgg aacaatatga gagagctgag ggcagacata gcacaggcgc tggataagtc
     2521 gacaatcaac ctctgattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2581 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg ttaactaaac
     2641 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2701 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     2761 catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctatc tggtctccct
     2821 tgagccatgc gtcaatttta cgcagactat ctttctaggg ttaaaggagc ctcgtgatac
     2881 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2941 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3001 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     3061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     3121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     3181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     3241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3301 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3481 gaggaccgaa ggagctaacc gcttttttgc acaacatg
//
