LOCUS       sequence-496980-        7165 bp    DNA     circular SYN 12-JAN-2026
DEFINITION  Poetur transposase expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Ivancic D, Agudelo A, Lindstrom-Vautrin J, Jaraba-Wallace J, Gallo 
            M, Das R, Ragel A, Herrero-Vicente J, Higueras I, Billeci F, 
            Sanvicente-Garcia M, Petazzi P, Ferruz N, Sanchez-Mejias A, Guell M
  TITLE     Discovery and protein language model-guided design of hyperactive 
            transposases.
  JOURNAL   Nat Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4.
  PUBMED    41039042
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 12, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 2. doi: 10.1038/s41587-025-02816-4."
COMMENT     Sequence Label: pCMV_PB_Poetur
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        360..563
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     514..534
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     608..627
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        608..626
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2463..2481)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2464..2481)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2501..2518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2507..2731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2777..3205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2864..2883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3074..3095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3214..3234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3219..3548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3399..3534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3461..3480
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3615..4409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3669..3688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4279..4298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4583..4704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4620..4639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4674..4693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4753..4769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4753..4769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4766..4788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4777..4793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4801..4807,4808..4825,4826..4831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4846..4867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4984..5001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5155..5743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5235..5254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5914..6705,6706..6774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6537..6556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6775..6879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6954..6973)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(7145..7165,1..359)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
       61 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      121 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      181 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      241 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      301 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      361 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      421 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      481 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      541 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      601 tcgaaattaa tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccact
      661 agtaacggcc gccagtgtgc tggaattcac cggtgtctat gagcagtaga cgattcactg
      721 ccgaggaagc ctctctgcag attctgaatt gggatagcga tgtagacgag gaaatatcag
      781 aaacagagga cccttcagaa ccggaggata acgctgcgga cgaccctgac tgtcattttt
      841 cacaggacga agaggattcc gaggatgata gctcctctga cgaaaatcaa gagactcagc
      901 agtcttcttc aactaaggga acatggactt ccaaggacgg gaaaatccgc tggtcttcat
      961 cacctcacca gagtcaggga agactcagct catctaacgt gatcaaaatg accccaggac
     1021 ccactcggtt cgctgtgacc cgcgtggacg acatcgagtc agcttttcag ctgttcataa
     1081 gtcccccgat cgaaaagatc atcctggaga tgactaacct ggaaggacgg agagtgttcc
     1141 aagagaaatg gaaacctttg gacccaacta acctccacgc ctatataggt atcctggtgt
     1201 tggcgggtgt gtaccgctcc aagggggaag ctactgcatc tctgtggaat gaagagaacg
     1261 gccgccccat tttccgagcg acaatgtcac tggaaacctt ccatatgata tcaagggtga
     1321 tcaggttcga taacagagat actagagcag ggcgtaggga acgcgacaag ttagccgcca
     1381 tccgcgacgt ttgggacaag tgggtcgaaa tcttacctct tctctataac ccaggcccac
     1441 atgttacagt cgatgagaga ctgatacctt tccggggccg gtgccctttt cgtcagtaca
     1501 tgcccaagaa gccagctaag tacgggatta aaatttgggc tgcctgcgat gccaagagct
     1561 catacgcctg gaatctgcag gtgtatacag gcaagcccgc tggcggtgcc cctgagaaaa
     1621 atcagggaat gcgggtcgtc ctcgagatga ccgagggact gcagggtcac aacatcacat
     1681 gtgataattt ctttaccagc tacagactgg gtgatgagct gcagaaacgt aaactgacca
     1741 tgcttggaac agttcgcaag aacaagcccg agctgcccag tgagctgttg atgatgaagg
     1801 gtaggcctct gcacagctcc aagtttgctt ttactgaaaa gacaaccctg gtctcttact
     1861 gccccaagcg gaataaaaat gtactcgtta tgtccactat gcataaggac gcctccctga
     1921 gcaccaggga agatatgaag cctcagatga tcctggatta taatagtacc aagggcggag
     1981 tggacaatct ggataaggta acagctacgt acagctgcca gcgcaagaca gcacgttggc
     2041 ccctggtgat cttttacaat atcgtagacg tttccgccta taatgcctac gtgctttgga
     2101 tcgagatcaa tcaacagtgg aatgccagca agttacaccg gaggaggctt tttctcgagg
     2161 agctcggtaa agcattggtg acaccacata tccaaaatcg agtccgccct gctcgaagct
     2221 tggctgcggc ggccgtgata gcaaaagtcc agggcagggc ctcagaccag cctgccatgg
     2281 aaccgttcga taccggggcc aagaaaagga agcgctgtca ggtttgctcc tctcgggacg
     2341 attctaagac tagtacttct tgtgtacggt gcaggaaata tatttgccgc aagcataccg
     2401 tgacattctg ccctagttgt ggagagagat aactgctcga gcatgcatct agagggccct
     2461 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     2521 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2581 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2641 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2701 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     2761 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2821 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2881 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2941 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3001 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3061 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3181 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3301 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3361 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3421 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3481 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3541 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     3601 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3661 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3721 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3781 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3841 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3901 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     3961 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4021 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4081 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4141 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4201 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4261 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4321 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4381 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     4441 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4501 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4561 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4621 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4681 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4741 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4801 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4861 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4921 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4981 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5041 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5101 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5161 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5221 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5281 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5341 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5401 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5461 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5521 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5581 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5641 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5701 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5761 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5821 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5881 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5941 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6001 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6061 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6121 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6181 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6241 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6301 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6361 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6421 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6481 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6541 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6601 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6661 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6721 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6781 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6841 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6901 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     6961 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     7021 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7081 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     7141 cgttgacatt gattattgac tagtt
//
