LOCUS       sequence-497315-        5831 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  An expression construct encoding a CIBN-fused STIM1 fragment 
            (a.a.238-685). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Kook YH, Lee H, Lee J, Jeong Y, Rho J, Heo WD, Lee S
  TITLE     AAV-compatible optogenetic tools for activating endogenous calcium 
            channels in vivo.
  JOURNAL   Mol Brain. 2023 Oct 17;16(1):73. doi: 10.1186/s13041-023-01061-7.
  PUBMED    37848907
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2023-10-17"; volume: "16(1):73. doi"; pages: " 
            10.1186/s13041-023-01061-7"
COMMENT     Sequence Label: pCMV-CIBN-STIM1(238-685)
FEATURES             Location/Qualifiers
     source          1..5831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             603..1112
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     polyA_signal    2619..2740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2656..2675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2710..2729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2747..3202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2884..2905)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3096..3115
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3229..3333
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3335..3692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3335..3526
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3358..3378)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3543..3678
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3727..4521
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3727..4521
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3781..3800)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4391..4410
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4709..4728)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4753..4800
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5129..5717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5618..5637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      601 ccatgaatgg agctatagga ggtgaccttt tgctcaattt tcctgacatg tcggtcctag
      661 agcgccaaag ggctcacctc aagtacctca atcccacctt tgattctcct ctcgccggct
      721 tctttgccga ttcttcaatg attaccggcg gcgagatgga cagctatctt tcgactgccg
      781 gtttgaatct tccgatgatg tacggtgaga cgacggtgga aggtgattca agactctcaa
      841 tttcgccgga aacgacgctt gggactggaa atttcaaggc agcgaagttt gatacagaga
      901 ctaaggattg taatgaggcg gcgaagaaga tgacgatgaa cagagatgac ctagtagaag
      961 aaggagaaga agagaagtcg aaaataacag agcaaaacaa tgggagcaca aaaagcatca
     1021 agaagatgaa acacaaagcc aagaaagaag agaacaattt ctctaatgat tcatctaaag
     1081 tgacgaagga attggagaaa acggattata ttgtgtacaa gtccggactc agatctaagg
     1141 agcacatgaa gaagatgatg aaggacttgg aggggttaca ccgagctgag cagagtctgc
     1201 atgaccttca ggaaaggctg cacaaggccc aggaggagca ccgcacagtg gaggtggaga
     1261 aggtccatct ggaaaagaag ctgcgcgatg agatcaacct tgctaagcag gaagcccagc
     1321 ggctgaagga gctgcgggag ggtactgaga atgagcggag ccgccaaaaa tatgctgagg
     1381 aggagttgga gcaggttcgg gaggccttga ggaaagcaga gaaggagcta gaatctcaca
     1441 gctcatggta tgctccagag gcccttcaga agtggctgca gctgacacat gaggtggagg
     1501 tgcaatatta caacatcaag aagcaaaatg ctgagaagca gctgctggtg gccaaggagg
     1561 gggctgagaa gataaaaaag aagagaaaca cactctttgg caccttccac gtggcccaca
     1621 gctcttccct ggatgatgta gatcataaaa ttctaacagc taagcaagca ctgagcgagg
     1681 tgacagcagc attgcgggag cgcctgcacc gctggcaaca gatcgagatc ctctgtggct
     1741 tccagattgt caacaaccct ggcatccact cactggtggc tgccctcaac atagacccca
     1801 gctggatggg cagtacacgc cccaaccctg ctcacttcat catgactgac gacgtggatg
     1861 acatggatga ggagattgtg tctcccttgt ccatgcagtc ccctagcctg cagagcagtg
     1921 ttcggcagcg cctgacggag ccacagcatg gcctgggatc tcagagagat ttgacccatt
     1981 ccgattcgga gtcctccctc cacatgagtg accgccagcg tgtggccccc aaacctcctc
     2041 agatgagccg tgctgcagac gaggctctca atgccatgac ttccaatggc agccaccggc
     2101 tgatcgaggg ggtccaccca gggtctctgg tggagaaact gcctgacagc cctgccctgg
     2161 ccaagaaggc attactggcg ctgaaccatg ggctggacaa ggcccacagc ctgatggagc
     2221 tgagcccctc agccccacct ggtggctctc cacatttgga ttcttcccgt tctcacagcc
     2281 ccagctcccc agacccagac acaccatctc cagttgggga cagccgagcc ctgcaagcca
     2341 gccgaaacac acgcattccc cacctggctg gcaagaaggc tgtggctgag gaggataatg
     2401 gctctattgg cgaggaaaca gactccagcc caggccggaa gaagtttccc ctcaaaatct
     2461 ttaagaagcc tcttaagaag taggaattgg gatccaccgg atctagataa ctgatcataa
     2521 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     2581 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     2641 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2701 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     2761 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     2821 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     2881 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     2941 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3001 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3061 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3121 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3181 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3301 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     3361 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3421 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     3481 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     3541 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3601 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3661 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     3721 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3781 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3841 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3901 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3961 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4021 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4081 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4141 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4201 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     4261 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4321 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4381 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4441 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4501 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4561 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4621 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4681 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     4741 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     4801 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     4861 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     4921 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     4981 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5041 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5101 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5161 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5221 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5281 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5341 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5401 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5461 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5521 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5581 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5641 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5701 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5761 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5821 ccgccatgca t
//
