LOCUS       sequence-497318-        6751 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 
            fragment (a.a.238-685) under the control of the CaMKII-alpha 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Kook YH, Lee H, Lee J, Jeong Y, Rho J, Heo WD, Lee S
  TITLE     AAV-compatible optogenetic tools for activating endogenous calcium 
            channels in vivo.
  JOURNAL   Mol Brain. 2023 Oct 17;16(1):73. doi: 10.1186/s13041-023-01061-7.
  PUBMED    37848907
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2023-10-17"; volume: "16(1):73. doi"; pages: " 
            10.1186/s13041-023-01061-7"
COMMENT     Sequence Label: pAAV-CaMKII-alpha-FLAG-STIM1(238-685)
FEATURES             Location/Qualifiers
     source          1..6751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      541..550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..576
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3614..3634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4014..4154
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4014..4143
                     /label=AAV2 ITR
     rep_origin      4229..4684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4316..4335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4526..4547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4701..4720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4820..4842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4880..4898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4966..5070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5071..5139,5140..5931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5289..5308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6102..6690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6591..6610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaacttg tggactaagt ttgttcacat ccccttctcc
      181 aaccccctca gtacatcacc ctgggggaac agggtccact tgctcctggg cccacacagt
      241 cctgcagtat tgtgtatata aggccagggc aaagaggagc aggttttaaa gtgaaaggca
      301 ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca gggcgtttcg gaggtggttg
      361 ccatggggac ctggatgctg acgaaggctc gcgaggctgt gagcagccac agtgccctgc
      421 tcagaagccc caagctcgtc agtcaagccg gttctccgtt tgcactcagg agcacgggca
      481 ggcgagtggc ccctagttct gggggcagct ctagagccga tccccgggta ccgggctagc
      541 gccaccatgg gagactacaa ggatgacgac gataagccac cggtcatgaa gatggacaaa
      601 aagaccatcg tctggtttcg gagagatttg agaatagaag ataatcccgc gctcgccgcc
      661 gcggcccacg agggttccgt cttccccgtt ttcatttggt gtcctgaaga agaaggccag
      721 ttttatcccg gaagggcctc taggtggtgg atgaagcaaa gtctggccca tcttagccag
      781 tcactgaaag cactgggcag tgatcttacc ctgatcaaga cacacaatac catctctgcc
      841 atcctcgact gcatcagggt gaccggcgca acgaaagtcg tgtttaacca cctgtacgat
      901 ccagttagtc tggtgcgcga ccacactgtg aaggagaagc tggtggaacg ggggatcagt
      961 gtgcagagct acaacgggga ccttctgtac gagccatggg agatctattg cgagaaaggg
     1021 aaaccgttca cctccttcaa cagttactgg aagaaatgtt tggatatgtc aatagagtcc
     1081 gttatgttgc cccctccctg gagactgatg ccgattactg ctgctgcaga ggccatctgg
     1141 gcctgctcca tcgaggaact cggtctggaa aatgaagcag aaaagccaag caatgcactt
     1201 ctcactagag cctggagccc cggctggtct aatgccgaca agctgcttaa cgagttcatc
     1261 gaaaaacaac tgattgacta cgcgaagaac tccaagaaag tggtaggtaa ctcaactagc
     1321 ttgctctctc catatctcca ttttggcgag atttctgtcc gccatgtatt tcagtgcgct
     1381 cggatgaaac agattatctg ggctcgcgat aaaaacagcg aaggcgccga aagcgccgat
     1441 ctgttcctgc gagggatcgg acttcgggaa tactcccggt atatatgttt caactttcca
     1501 ttcacacacg agcagagtct gttgtcccac ctcaggttct tcccctggga cgccgatgtc
     1561 gacaaattca aggcatggag acagggaagg acagggtacc cactcgtgga tgctggcatg
     1621 agagagctct gggctacagg ctggatgcac aaccgcatcc gggtaatcgt gtcctcattt
     1681 gctgtcaagt ttctgctcct gccttggaaa tggggaatga agtacttttg ggataccctt
     1741 ctcgacgccg acttggagtg tgacattctg ggatggcaat atattagcgg gtcaattcct
     1801 gacggccatg agttggacag gttggacaat ccggccttgc agggagctaa gtatgatccc
     1861 gaaggagagt atattcgaca gtggctcccc gagctggccc gacttcctac ggagtggatt
     1921 caccatcctt gggacgcacc actgacagtg ctcaaggcaa gcggggtgga gctgggcacc
     1981 aattacgcta agcctatagt tgatatagat acagcacgcg agctgctggc taaagcgatc
     2041 tctcgcactc gggaggcgca gattatgatc ggtgctgccg ccagggaccc ccccgacctc
     2101 gacaacgaat tcagtggggg aggcggaggg ggtggttcag gtggtggtgg tggttcgggt
     2161 ggtggcggag ggggaggctc cggactcaga tctaaggagc acatgaagaa gatgatgaag
     2221 gacttggagg ggttacaccg agctgagcag agtctgcatg accttcagga aaggctgcac
     2281 aaggcccagg aggagcaccg cacagtggag gtggagaagg tccatctgga aaagaagctg
     2341 cgcgatgaga tcaaccttgc taagcaggaa gcccagcggc tgaaggagct gcgggagggt
     2401 actgagaatg agcggagccg ccaaaaatat gctgaggagg agttggagca ggttcgggag
     2461 gccttgagga aagcagagaa ggagctagaa tctcacagct catggtatgc tccagaggcc
     2521 cttcagaagt ggctgcagct gacacatgag gtggaggtgc aatattacaa catcaagaag
     2581 caaaatgctg agaagcagct gctggtggcc aaggaggggg ctgagaagat aaaaaagaag
     2641 agaaacacac tctttggcac cttccacgtg gcccacagct cttccctgga tgatgtagat
     2701 cataaaattc taacagctaa gcaagcactg agcgaggtga cagcagcatt gcgggagcgc
     2761 ctgcaccgct ggcaacagat cgagatcctc tgtggcttcc agattgtcaa caaccctggc
     2821 atccactcac tggtggctgc cctcaacata gaccccagct ggatgggcag tacacgcccc
     2881 aaccctgctc acttcatcat gactgacgac gtggatgaca tggatgagga gattgtgtct
     2941 cccttgtcca tgcagtcccc tagcctgcag agcagtgttc ggcagcgcct gacggagcca
     3001 cagcatggcc tgggatctca gagagatttg acccattccg attcggagtc ctccctccac
     3061 atgagtgacc gccagcgtgt ggcccccaaa cctcctcaga tgagccgtgc tgcagacgag
     3121 gctctcaatg ccatgacttc caatggcagc caccggctga tcgagggggt ccacccaggg
     3181 tctctggtgg agaaactgcc tgacagccct gccctggcca agaaggcatt actggcgctg
     3241 aaccatgggc tggacaaggc ccacagcctg atggagctga gcccctcagc cccacctggt
     3301 ggctctccac atttggattc ttcccgttct cacagcccca gctccccaga cccagacaca
     3361 ccatctccag ttggggacag ccgagccctg caagccagcc gaaacacacg cattccccac
     3421 ctggctggca agaaggctgt ggctgaggag gataatggct ctattggcga ggaaacagac
     3481 tccagcccag gccggaagaa gtttcccctc aaaatcttta agaagcctct taagaagtag
     3541 gaattgggat ccctcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3601 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3661 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
     3721 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3781 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
     3841 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3901 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3961 ggggagatgt gggaggtttt ttaaagcggc cgcaggacgg accgagcggc cgcaggaacc
     4021 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4081 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4141 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4201 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4441 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4561 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     4621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4681 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4741 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4801 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4861 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4921 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4981 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5041 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5101 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5161 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5221 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5281 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5341 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5401 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5461 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5521 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5581 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5641 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5701 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5761 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5821 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5881 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5941 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6001 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6061 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6121 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6181 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6241 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6301 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6361 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6421 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6481 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6541 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6601 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6661 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6721 ctggcctttt gctggccttt tgctcacatg t
//
