LOCUS       sequence-497321-        7067 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  An AAV construct designed to express a CRY2-fused FLAG-tagged STIM1 
            fragment (a.a.238-685) under the control of the GfaABC1D promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Kook YH, Lee H, Lee J, Jeong Y, Rho J, Heo WD, Lee S
  TITLE     AAV-compatible optogenetic tools for activating endogenous calcium 
            channels in vivo.
  JOURNAL   Mol Brain. 2023 Oct 17;16(1):73. doi: 10.1186/s13041-023-01061-7.
  PUBMED    37848907
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Brain."; date: "2023-10-17"; volume: "16(1):73. doi"; pages: " 
            10.1186/s13041-023-01061-7"
COMMENT     Sequence Label: pAAV-GfaABC1D-FLAG-STIM1(238-685)
FEATURES             Location/Qualifiers
     source          1..7067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   354..494
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   354..483
                     /label=AAV2 ITR
     rep_origin      569..1024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(656..675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     866..887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1041..1060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1160..1182
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1220..1238)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1306..1410
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1411..1479,1480..2271)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2442..3030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2931..2950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   3092..3221
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3948..3957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3960..3983
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7021..7041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
       61 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
      121 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
      181 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      241 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      301 ggggagatgt gggaggtttt ttaaagcggc cgcaggacgg accgagcggc cgcaggaacc
      361 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
      421 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
      481 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      541 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
      601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
      661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
      721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
      781 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
      841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
      901 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
      961 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     1021 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     1081 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     1141 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1201 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1261 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1321 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1381 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1441 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1501 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1561 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1621 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1681 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1741 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1801 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1861 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1921 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1981 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2041 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2101 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2161 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2221 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2281 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2341 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2461 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2521 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2581 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2641 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2701 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2761 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2821 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2881 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2941 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3001 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3061 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     3121 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     3181 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtaacat
     3241 atcctggtgt ggagtagggg acgctgctct gacagaggct cgggggcctg agctggctct
     3301 gtgagctggg gaggaggcag acagccaggc cttgtctgca agcagacctg gcagcattgg
     3361 gctggccgcc ccccagggcc tcctcttcat gcccagtgaa tgactcacct tggcacagac
     3421 acaatgttcg gggtgggcac agtgcctgct tcccgccgca ccccagcccc cctcaaatgc
     3481 cttccgagaa gcccattgag cagggggctt gcattgcacc ccagcctgac agcctggcat
     3541 cttgggataa aagcagcaca gccccctagg ggctgccctt gctgtgtggc gccaccggcg
     3601 gtggagaaca aggctctatt cagcctgtgc ccaggaaagg ggatcagggg atgcccaggc
     3661 atggacagtg ggtggcaggg ggggagagga gggctgtctg cttcccagaa gtccaaggac
     3721 acaaatgggt gaggggagag ctctccccat agctgggctg cggcccaacc ccaccccctc
     3781 aggctatgcc agggggtgtt gccaggggca cccgggcatc gccagtctag cccactcctt
     3841 cacaaagccc tcgcatccca ggagcgagca gagccagagc aggttggaga ggagacgcat
     3901 cacctccgct gctcgctcta gagccgatcc ccgggtaccg ggctagcgcc accatgggag
     3961 actacaagga tgacgacgat aagccaccgg tcatgaagat ggacaaaaag accatcgtct
     4021 ggtttcggag agatttgaga atagaagata atcccgcgct cgccgccgcg gcccacgagg
     4081 gttccgtctt ccccgttttc atttggtgtc ctgaagaaga aggccagttt tatcccggaa
     4141 gggcctctag gtggtggatg aagcaaagtc tggcccatct tagccagtca ctgaaagcac
     4201 tgggcagtga tcttaccctg atcaagacac acaataccat ctctgccatc ctcgactgca
     4261 tcagggtgac cggcgcaacg aaagtcgtgt ttaaccacct gtacgatcca gttagtctgg
     4321 tgcgcgacca cactgtgaag gagaagctgg tggaacgggg gatcagtgtg cagagctaca
     4381 acggggacct tctgtacgag ccatgggaga tctattgcga gaaagggaaa ccgttcacct
     4441 ccttcaacag ttactggaag aaatgtttgg atatgtcaat agagtccgtt atgttgcccc
     4501 ctccctggag actgatgccg attactgctg ctgcagaggc catctgggcc tgctccatcg
     4561 aggaactcgg tctggaaaat gaagcagaaa agccaagcaa tgcacttctc actagagcct
     4621 ggagccccgg ctggtctaat gccgacaagc tgcttaacga gttcatcgaa aaacaactga
     4681 ttgactacgc gaagaactcc aagaaagtgg taggtaactc aactagcttg ctctctccat
     4741 atctccattt tggcgagatt tctgtccgcc atgtatttca gtgcgctcgg atgaaacaga
     4801 ttatctgggc tcgcgataaa aacagcgaag gcgccgaaag cgccgatctg ttcctgcgag
     4861 ggatcggact tcgggaatac tcccggtata tatgtttcaa ctttccattc acacacgagc
     4921 agagtctgtt gtcccacctc aggttcttcc cctgggacgc cgatgtcgac aaattcaagg
     4981 catggagaca gggaaggaca gggtacccac tcgtggatgc tggcatgaga gagctctggg
     5041 ctacaggctg gatgcacaac cgcatccggg taatcgtgtc ctcatttgct gtcaagtttc
     5101 tgctcctgcc ttggaaatgg ggaatgaagt acttttggga tacccttctc gacgccgact
     5161 tggagtgtga cattctggga tggcaatata ttagcgggtc aattcctgac ggccatgagt
     5221 tggacaggtt ggacaatccg gccttgcagg gagctaagta tgatcccgaa ggagagtata
     5281 ttcgacagtg gctccccgag ctggcccgac ttcctacgga gtggattcac catccttggg
     5341 acgcaccact gacagtgctc aaggcaagcg gggtggagct gggcaccaat tacgctaagc
     5401 ctatagttga tatagataca gcacgcgagc tgctggctaa agcgatctct cgcactcggg
     5461 aggcgcagat tatgatcggt gctgccgcca gggacccccc cgacctcgac aacgaattca
     5521 gtgggggagg cggagggggt ggttcaggtg gtggtggtgg ttcgggtggt ggcggagggg
     5581 gaggctccgg actcagatct aaggagcaca tgaagaagat gatgaaggac ttggaggggt
     5641 tacaccgagc tgagcagagt ctgcatgacc ttcaggaaag gctgcacaag gcccaggagg
     5701 agcaccgcac agtggaggtg gagaaggtcc atctggaaaa gaagctgcgc gatgagatca
     5761 accttgctaa gcaggaagcc cagcggctga aggagctgcg ggagggtact gagaatgagc
     5821 ggagccgcca aaaatatgct gaggaggagt tggagcaggt tcgggaggcc ttgaggaaag
     5881 cagagaagga gctagaatct cacagctcat ggtatgctcc agaggccctt cagaagtggc
     5941 tgcagctgac acatgaggtg gaggtgcaat attacaacat caagaagcaa aatgctgaga
     6001 agcagctgct ggtggccaag gagggggctg agaagataaa aaagaagaga aacacactct
     6061 ttggcacctt ccacgtggcc cacagctctt ccctggatga tgtagatcat aaaattctaa
     6121 cagctaagca agcactgagc gaggtgacag cagcattgcg ggagcgcctg caccgctggc
     6181 aacagatcga gatcctctgt ggcttccaga ttgtcaacaa ccctggcatc cactcactgg
     6241 tggctgccct caacatagac cccagctgga tgggcagtac acgccccaac cctgctcact
     6301 tcatcatgac tgacgacgtg gatgacatgg atgaggagat tgtgtctccc ttgtccatgc
     6361 agtcccctag cctgcagagc agtgttcggc agcgcctgac ggagccacag catggcctgg
     6421 gatctcagag agatttgacc cattccgatt cggagtcctc cctccacatg agtgaccgcc
     6481 agcgtgtggc ccccaaacct cctcagatga gccgtgctgc agacgaggct ctcaatgcca
     6541 tgacttccaa tggcagccac cggctgatcg agggggtcca cccagggtct ctggtggaga
     6601 aactgcctga cagccctgcc ctggccaaga aggcattact ggcgctgaac catgggctgg
     6661 acaaggccca cagcctgatg gagctgagcc cctcagcccc acctggtggc tctccacatt
     6721 tggattcttc ccgttctcac agccccagct ccccagaccc agacacacca tctccagttg
     6781 gggacagccg agccctgcaa gccagccgaa acacacgcat tccccacctg gctggcaaga
     6841 aggctgtggc tgaggaggat aatggctcta ttggcgagga aacagactcc agcccaggcc
     6901 ggaagaagtt tcccctcaaa atctttaaga agcctcttaa gaagtaggaa ttgggatccc
     6961 tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7021 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgt
//
