LOCUS       sequence-497339-        7746 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  Fluorescence reporter for intracellular distribution of BMAL1 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7746)
  TITLE     Immediate nuclear accumulation of BMAL1 to regulate cellular 
            circadian clock synchronization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMX Bmal1pomoter mVenus-linkerA-BMAL1
FEATURES             Location/Qualifiers
     source          1..7746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..601
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    664..1021
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1086..1502
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1432..1454
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1470..1486
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1512..1886
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1824..1840
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2401..2410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2407..2409,2410..2412,2413..3123)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2452..2473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2713..2732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3060..3081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5237..5706
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5836..5853)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6007..6595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6087..6106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6759..7550,7551..7619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7382..7401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7620..7724)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgcataat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag
      121 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      181 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      301 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      361 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
      421 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag
      481 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
      601 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
      661 ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
      721 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
      781 aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg
      841 ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac
      901 cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
      961 ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct
     1021 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatgg
     1081 gcccgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     1141 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     1201 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     1261 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     1321 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     1381 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     1441 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     1501 cccccatatg gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg
     1561 accctgacat gacaagagtt actaacagcc cctctctcca agctcactta caggctctct
     1621 acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc
     1681 gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga
     1741 ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg
     1801 ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc
     1861 ccgggggtgg accatcctct agactgccgg atccgggacg acggcgagct cgcagagtcc
     1921 gcaacgcagt ggcctcagcg agctttagac ctgaggggga aaaccgaggg ctggggcaag
     1981 aaatccacag agcgtgccaa ttggtccact cctcggggcg tgtgcttctg tgcgccaaat
     2041 gattggtgaa gggaaagtag caggtaaacc agccctgccg tcttgccatt ggtcagaggc
     2101 tttcctatcg gtcactcgat tggctagcct aacgcagagc agaacgcgaa ttggtttggg
     2161 ttgtccgcca agacaactcc gttcgctctc tctgattggc taacgggaag aggcaggtat
     2221 ccgggctgcg cggctcctcc attggtgggc ggggaagggg ggttgggcac agcgattggt
     2281 gggcgggggg ccgggcctgg gccggcgggg agcggattgg tcggaaagta ggttagtggt
     2341 gcgacattta gggaaggcag aaagtaggtc agggacggag gtgcctgttt acccagatct
     2401 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2461 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2521 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
     2581 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
     2641 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2701 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2761 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2821 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     2881 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     2941 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3001 aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac
     3061 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3121 aagggctccg ctggctccgc tgctggttct ggcgaatttg aattcatggc ggaccagaga
     3181 atggacattt cctcaaccat cagcgacttc atgtctccgg gccccaccga cctactctcc
     3241 ggttccctgg gcaccagtgg tgtggactgc aatcgcaaga ggaaaggcag tgccactgac
     3301 taccaagaaa gtatggacac agacaaagat gaccctcatg gaaggttaga atatgcagaa
     3361 caccaaggaa ggatcaagaa tgcaagggag gcccacagtc agattgaaaa gaggcgtcgg
     3421 gacaaaatga acagtttcat tgatgaattg gcttctttgg taccaacatg caatgcaatg
     3481 tccaggaagt tagataaact caccgtgcta aggatggctg ttcagcacat gaaaactttg
     3541 agaggtgcca ccaacccata cacagaagca aactacaagc caacatttct atcagatgac
     3601 gaactgaaac acctaattct cagggcagca gatggatttt tgtttgtcgt aggatgtgac
     3661 cgagggaaga tcctctttgt ctccgagtct gtcttcaaga tcctcaatta tagccagaat
     3721 gaccttattg gccagagctt gtttgactac ctgcatccaa aagatattgc caaagttaag
     3781 gaacagctat cttcctcgga cactgcgccc cgggagcgac tcattgatgc caagactgga
     3841 cttccggtta aaacggatat aacccctggg ccctcccggc tatgctctgg agcccgccgc
     3901 tctttcttct gtagaatgaa gtgcaacagg ccttcagtaa aggtggaaga taaggacttc
     3961 gcctctacct gttcaaagaa aaaagatcga aaaagcttct gcacaatcca cagcacaggc
     4021 tatttgaaaa gctggccacc cacgaagatg gggctggacg aagacaatga gccagacaac
     4081 gagggctgca acctcagctg cctcgttgca atcgggcgcc tgcactcgca catggttcca
     4141 caaccagcga acggggaaat acgggtgaaa tctatggagt acgtttctcg acacgcaata
     4201 gatgggaaat ttgtttttgt agatcagagg gcgacagcta ttttggcgta tctaccacag
     4261 gaacttctag gtacatcatg ttatgagtat tttcatcaag acgacatagg acacctcgca
     4321 gaatgtcaca ggcaagtttt acagacaaga gaaaagatca caactaattg ctataagttt
     4381 aagatcaaag atggttcttt tatcacgcta cgaagtcgat ggttcagttt catgaacccg
     4441 tggaccaagg aagttgaata cattgtctca accaacactg ttgttttagc caatgtcctg
     4501 gaaggcgggg acccaacctt cccgcagcta acagcacccc cccacagcat ggacagcatg
     4561 ctgccctctg gagaaggtgg cccaaagagg actcatccca ctgttccagg cattccaggg
     4621 ggaaccagag ccggagcagg aaaaataggt cgaatgattg ccgaggaaat catggaaatc
     4681 cacaggataa gagggtcatc gccttccagc tgtggctcca gcccgctgaa catcacaagt
     4741 acgcctcccc ctgatgcccc ttctccagga ggcaagaaga ttctaaatgg agggactcca
     4801 gacattcctt ccactggact attaccaggg caggctcagg agaccccagg gtatccctat
     4861 tctgatagtt cttctattct tggtgagaac ccccacatag gcatcgatat gatagataac
     4921 gaccaaggat caagtagtcc cagtaacgat gaggcagcaa tggctgtcat catgagcctc
     4981 ttggaagcag atgcggggct gggtggcccc gttgacttta gtgacttgcc atggccgctg
     5041 tagctcgagc ggccgccagc acagtggtcg acgataaaat aaaagatttt atttagtctc
     5101 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     5161 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     5221 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     5281 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     5341 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     5401 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     5461 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     5521 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     5581 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     5641 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     5701 ctttcacatg cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa
     5761 atggccatag ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6421 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgcggc cggccgcaaa
     6721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7741 gtgcca
//
