LOCUS       sequence-497344-        7760 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  Fluorescence reporter for intracellular distribution of BMAL1 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7760)
  TITLE     Immediate nuclear accumulation of BMAL1 to regulate cellular 
            circadian clock synchronization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMX Bmal1pomoter mVenus-linkerB-BMAL1
FEATURES             Location/Qualifiers
     source          1..7760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..601
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    664..1021
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1086..1502
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1432..1454
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1470..1486
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1512..1886
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1824..1840
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2397..2406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2403..2405,2406..2408,2409..3119)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2448..2469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2709..2728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3056..3077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5251..5720
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5850..5867)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6021..6609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6101..6120)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6773..7564,7565..7633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7396..7415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7634..7738)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgcataat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag
      121 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      181 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      301 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      361 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
      421 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag
      481 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
      601 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
      661 ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
      721 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
      781 aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg
      841 ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac
      901 cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
      961 ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct
     1021 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatgg
     1081 gcccgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     1141 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     1201 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     1261 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     1321 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     1381 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     1441 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     1501 cccccatatg gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg
     1561 accctgacat gacaagagtt actaacagcc cctctctcca agctcactta caggctctct
     1621 acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc
     1681 gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga
     1741 ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg
     1801 ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc
     1861 ccgggggtgg accatcctct agactgccgg atccgggacg acggcgagct cgcagagtcc
     1921 gcaacgcagt ggcctcagcg agctttagac ctgaggggga aaaccgaggg ctggggcaag
     1981 aaatccacag agcgtgccaa ttggtccact cctcggggcg tgtgcttctg tgcgccaaat
     2041 gattggtgaa gggaaagtag caggtaaacc agccctgccg tcttgccatt ggtcagaggc
     2101 tttcctatcg gtcactcgat tggctagcct aacgcagagc agaacgcgaa ttggtttggg
     2161 ttgtccgcca agacaactcc gttcgctctc tctgattggc taacgggaag aggcaggtat
     2221 ccgggctgcg cggctcctcg gtgggcgggg aaggggggtt gggcacagcg attggtgggc
     2281 ggggggccgg gcctgggccg gcggggagcg gattggtcgg aaagtaggtt agtggtgcga
     2341 catttaggga aggcagaaag taggtcaggg acggaggtgc ctgtttaccc agatctgcca
     2401 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2461 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2521 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
     2581 ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
     2641 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2701 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2761 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2821 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
     2881 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
     2941 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3001 actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc gatcacatgg
     3061 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     3121 gtggctctgg cggaagcggc tccgctggct ctgctgccgg atctggcgag tttgaattca
     3181 tggcggacca gagaatggac atttcctcaa ccatcagcga cttcatgtct ccgggcccca
     3241 ccgacctact ctccggttcc ctgggcacca gtggtgtgga ctgcaatcgc aagaggaaag
     3301 gcagtgccac tgactaccaa gaaagtatgg acacagacaa agatgaccct catggaaggt
     3361 tagaatatgc agaacaccaa ggaaggatca agaatgcaag ggaggcccac agtcagattg
     3421 aaaagaggcg tcgggacaaa atgaacagtt tcattgatga attggcttct ttggtaccaa
     3481 catgcaatgc aatgtccagg aagttagata aactcaccgt gctaaggatg gctgttcagc
     3541 acatgaaaac tttgagaggt gccaccaacc catacacaga agcaaactac aagccaacat
     3601 ttctatcaga tgacgaactg aaacacctaa ttctcagggc agcagatgga tttttgtttg
     3661 tcgtaggatg tgaccgaggg aagatcctct ttgtctccga gtctgtcttc aagatcctca
     3721 attatagcca gaatgacctt attggccaga gcttgtttga ctacctgcat ccaaaagata
     3781 ttgccaaagt taaggaacag ctatcttcct cggacactgc gccccgggag cgactcattg
     3841 atgccaagac tggacttccg gttaaaacgg atataacccc tgggccctcc cggctatgct
     3901 ctggagcccg ccgctctttc ttctgtagaa tgaagtgcaa caggccttca gtaaaggtgg
     3961 aagataagga cttcgcctct acctgttcaa agaaaaaaga tcgaaaaagc ttctgcacaa
     4021 tccacagcac aggctatttg aaaagctggc cacccacgaa gatggggctg gacgaagaca
     4081 atgagccaga caacgagggc tgcaacctca gctgcctcgt tgcaatcggg cgcctgcact
     4141 cgcacatggt tccacaacca gcgaacgggg aaatacgggt gaaatctatg gagtacgttt
     4201 ctcgacacgc aatagatggg aaatttgttt ttgtagatca gagggcgaca gctattttgg
     4261 cgtatctacc acaggaactt ctaggtacat catgttatga gtattttcat caagacgaca
     4321 taggacacct cgcagaatgt cacaggcaag ttttacagac aagagaaaag atcacaacta
     4381 attgctataa gtttaagatc aaagatggtt cttttatcac gctacgaagt cgatggttca
     4441 gtttcatgaa cccgtggacc aaggaagttg aatacattgt ctcaaccaac actgttgttt
     4501 tagccaatgt cctggaaggc ggggacccaa ccttcccgca gctaacagca cccccccaca
     4561 gcatggacag catgctgccc tctggagaag gtggcccaaa gaggactcat cccactgttc
     4621 caggcattcc agggggaacc agagccggag caggaaaaat aggtcgaatg attgccgagg
     4681 aaatcatgga aatccacagg ataagagggt catcgccttc cagctgtggc tccagcccgc
     4741 tgaacatcac aagtacgcct ccccctgatg ccccttctcc aggaggcaag aagattctaa
     4801 atggagggac tccagacatt ccttccactg gactattacc agggcaggct caggagaccc
     4861 cagggtatcc ctattctgat agttcttcta ttcttggtga gaacccccac ataggcatcg
     4921 atatgataga taacgaccaa ggatcaagta gtcccagtaa cgatgaggca gcaatggctg
     4981 tcatcatgag cctcttggaa gcagatgcgg ggctgggtgg ccccgttgac tttagtgact
     5041 tgccatggcc gctgtagctc gagcggccgc cagcacagtg gtcgacgata aaataaaaga
     5101 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     5161 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     5221 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     5281 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
     5341 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     5401 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     5461 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     5521 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
     5581 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     5641 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     5701 ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta
     5761 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc
     5821 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6181 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6421 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
     6721 cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7081 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7741 catttccccg aaaagtgcca
//
