LOCUS       sequence-497345-        5686 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  Fluorescence nuclear marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5686)
  TITLE     Immediate nuclear accumulation of BMAL1 to regulate cellular 
            circadian clock synchronization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMX H2B-mKate2
FEATURES             Location/Qualifiers
     source          1..5686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..601
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    664..1021
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1086..1502
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1432..1454
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1470..1486
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1512..1886
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1824..1840
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2285..2977
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGDPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     LTR             3177..3646
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3776..3793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3947..4535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4027..4046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4699..5490,5491..5559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5322..5341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5560..5664)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgcataat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
       61 gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag
      121 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      181 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      301 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      361 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
      421 gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag
      481 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
      601 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
      661 ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt
      721 tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg
      781 aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg
      841 ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac
      901 cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
      961 ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct
     1021 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaatatgg
     1081 gcccgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     1141 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     1201 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     1261 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     1321 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     1381 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     1441 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     1501 cccccatatg gccatatgag atcttatatg gggcaccccc gccccttgta aacttccctg
     1561 accctgacat gacaagagtt actaacagcc cctctctcca agctcactta caggctctct
     1621 acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa caactggacc
     1681 gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga
     1741 ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg
     1801 ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc
     1861 ccgggggtgg accatcctct agactgccgg atccgccacc atgccggagc cagcgaagtc
     1921 tgctcccgcg cccaagaagg gctcgaagaa agccgtgact aaggcgcaga agaaggacgg
     1981 caagaagcgc aagcgcagcc gcaaggagag ctactccgta tacgtgtaca aggtgctgaa
     2041 gcaggtccac cccgacaccg gcatctcctc taaggccatg ggaatcatga actccttcgt
     2101 caacgacatc ttcgaacgca tcgcgggtga ggcttcccgc ctggcgcatt acaacaagcg
     2161 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ccggggagtt
     2221 ggccaagcac gccgtgtccg agggcaccaa ggccgtcacc aagtacacca gcgctaagga
     2281 attcgtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     2341 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     2401 gaccatgaga atcaaggcgg tcgagggcga ccctctcccc ttcgccttcg acatcctggc
     2461 taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt
     2521 ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat acgaagacgg
     2581 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     2641 caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
     2701 ctgggaggcc tccaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagagccga
     2761 catggccctg aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag
     2821 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact
     2881 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc
     2941 cagatactgc gacctcccta gcaaactggg gcacagaggc agcctcgagc ggccgccagc
     3001 acagtggtcg acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     3061 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
     3121 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
     3181 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     3241 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3301 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
     3361 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
     3421 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
     3481 gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
     3541 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
     3601 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
     3661 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
     3721 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4381 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4621 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
     4681 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4741 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4801 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4861 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4921 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4981 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5041 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5101 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5161 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5221 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5281 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5341 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5401 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5461 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5521 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5581 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5641 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgcca
//
