LOCUS       sequence-497372-        4499 bp    DNA     circular SYN 14-JAN-2026
DEFINITION  Recombinant protein synthesis of 2N3R isoform of human Tau.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4499)
  TITLE     Efficient Tag-less Purification of Human Tau Proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 14, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7II-2N3Rtau
FEATURES             Location/Qualifiers
     source          1..4499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..34)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        102..206
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(207..275,276..1067)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRHIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(425..444)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1238..1826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1727..1746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1980..1997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2012..2151
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2077..2096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2237..2259
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2495..2514
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2598..2617
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2598..2616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2648..2670
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     rep_origin      complement(3976..4488)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4170..4191)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4382..4401
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
       61 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      121 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      181 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      241 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      301 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      361 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      421 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
      481 acatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      541 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      601 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      661 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      721 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
      781 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      841 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      901 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      961 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1021 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1081 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1141 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1201 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1261 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1321 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1381 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1441 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1501 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1561 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1621 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1681 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1741 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1801 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1861 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1921 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1981 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     2041 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     2101 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2281 cgcgcgaggc agtcgatcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     2341 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     2401 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     2461 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     2521 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     2581 tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag
     2641 aaataatttt gtttaacttt aagaaggaga tataccatgg ctgagccccg ccaggagttc
     2701 gaagtgatgg aagatcacgc tgggacgtac gggttggggg acaggaaaga tcaggggggc
     2761 tacaccatgc accaagacca agagggtgac acggacgctg gcctgaaaga atctcccctg
     2821 cagaccccca ctgaggacgg atctgaggaa ccgggctctg aaacctctga tgctaagagc
     2881 actccaacag cggaagatgt gacagcaccc ttagtggatg agggagctcc cggcaagcag
     2941 gctgccgcgc agccccacac ggagatccca gaaggaacca cagctgaaga agcaggcatt
     3001 ggagacaccc ccagcctgga agacgaagct gctggtcacg tgacccaagc tcgcatggtc
     3061 agtaaaagca aagacgggac tggaagcgat gacaaaaaag ccaagggggc tgatggtaaa
     3121 acgaagatcg ccacaccgcg gggagcagcc cctccaggcc agaagggcca ggccaacgcc
     3181 accaggattc cagcaaaaac cccgcccgct ccaaagacac cacccagctc tggtgaacct
     3241 ccaaaatcag gggatcgcag cggctacagc agccccggct ccccaggcac tcccggcagc
     3301 cgctcccgca ccccgtccct tccaacccca cccacccggg agcccaagaa ggtggcagtg
     3361 gtccgtactc cacccaagtc gccgtcttcc gccaagagcc gcctgcagac agcccccgtg
     3421 cccatgccag acctgaagaa tgtcaagtcc aagatcggct ccactgagaa cctgaagcac
     3481 cagccgggag gcgggaaggt gcaaatagtc tacaaaccag ttgacctgag caaggtgacc
     3541 tccaagtgtg gctcattagg caacatccat cataaaccag gaggtggcca ggtggaagta
     3601 aaatctgaga agcttgactt caaggacaga gtccagtcga agattgggtc cctggacaat
     3661 atcacccacg tccctggcgg aggaaataaa aagattgaaa cccacaagct gaccttccgc
     3721 gagaacgcca aagccaagac agaccacggg gcggagatcg tgtacaagtc gccagtggtg
     3781 tctggggaca cgtctccacg gcatctcagc aatgtctcct ccaccggcag catcgacatg
     3841 gtagactcgc cccagctcgc cacgctagct gacgaggtgt ctgcctccct ggccaagcag
     3901 ggtttgtaag aattcaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct
     3961 gagttggctg ctgccacccc ggttgataat cagaaaagcc ccaaaaacag gaagattgta
     4021 taagcaaata tttaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4081 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4141 gaatagaccg agatagggtt gagtttgttc cagtttggaa caagagtcca ctattaaaga
     4201 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4261 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4321 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     4381 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     4441 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggatcaatt
//
