LOCUS       sequence-497404-       12149 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  Expression of GLI1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Krantz S, Bell BA, Lund K, Salinas Parra N, Ng Y, De Oliveira Rosa 
            N, Mukhopadhyay S, St Croix B, Sarin KY, Weigert R, Raimondi F, 
            Iglesias-Bartolome R
  TITLE     Dissection of Galphas and Hedgehog Signaling Crosstalk Reveals 
            Therapeutic Opportunities to Target Hedgehog-Dependent Tumors.
  JOURNAL   Cancer Res. 2025 Dec 29. doi: 10.1158/0008-5472.CAN-25-1109.
  PUBMED    41460725
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2025 Dec 29. doi: 10.1158/0008-5472.CAN-25-1109."
COMMENT     Sequence Label: pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1
FEATURES             Location/Qualifiers
     source          1..12149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3171..3197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6153..6177)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6195..6783
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6248..6268)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6666..6677)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        6813..7192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7193..7396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7347..7367
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7423..7432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7429..8136
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7574..7592)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7842..7861
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8146..8199
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8200..8598
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8670..8903
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8975..9096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9012..9031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9066..9085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9136..9271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9291..9310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9292..9310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9320..9337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9320..9336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9329..9351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9478..9933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9565..9584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9775..9796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9959..10063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10064..10132,10133..10924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10282..10301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11095..11683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11584..11603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11837..11854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11971..11992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12007..12012,12013..12030,12031..12037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12045..12061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12050..12072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12069..12085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12069..12085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12104..12124
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12106..12124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tacccatacg
     3181 atgttccaga ttacgctggc agcggcagcg gcagcatgag cccatctctg ggattcccag
     3241 cccagatgaa tcaccaaaaa gggccctcgc cttcctttgg ggtccagcct tgtggtcccc
     3301 atgactctgc ccggggtggg atgatcccac atcctcagtc ccggggaccc ttcccaactt
     3361 gccagctgaa gtctgagctg gacatgctgg ttggcaagtg ccgggaggaa cccttggaag
     3421 gtgatatgtc cagccccaac tccacaggca tacaggatcc cctgttgggg atgctggatg
     3481 ggcgggagga cctcgagaga gaggagaagc gtgagcctga atctgtgtat gaaactgact
     3541 gccgttggga tggctgcagc caggaatttg actcccaaga gcagctggtg caccacatca
     3601 acagcgagca catccacggg gagcggaagg agttcgtgtg ccactggggg ggctgctcca
     3661 gggagctgag gcccttcaaa gcccagtaca tgctggtggt tcacatgcgc agacacactg
     3721 gcgagaagcc acacaagtgc acgtttgaag ggtgccggaa gtcatactca cgcctcgaaa
     3781 acctgaagac gcacctgcgg tcacacacgg gtgagaagcc atacatgtgt gagcacgagg
     3841 gctgcagtaa agccttcagc aatgccagtg accgagccaa gcaccagaat cggacccatt
     3901 ccaatgagaa gccgtatgta tgtaagctcc ctggctgcac caaacgctat acagatccta
     3961 gctcgctgcg aaaacatgtc aagacagtgc atggtcctga cgcccatgtg accaaacggc
     4021 accgtgggga tggccccctg cctcgggcac catccatttc tacagtggag cccaagaggg
     4081 agcgggaagg aggtcccatc agggaggaaa gcagactgac tgtgccagag ggtgccatga
     4141 agccacagcc aagccctggg gcccagtcat cctgcagcag tgaccactcc ccggcaggga
     4201 gtgcagccaa tacagacagt ggtgtggaaa tgactggcaa tgcagggggc agcactgaag
     4261 acctctccag cttggacgag ggaccttgca ttgctggcac tggtctgtcc actcttcgcc
     4321 gccttgagaa cctcaggctg gaccagctac atcaactccg gccaataggg acccggggtc
     4381 tcaaactgcc cagcttgtcc cacaccggta ccactgtgtc ccgccgcgtg ggccccccag
     4441 tctctcttga acgccgcagc agcagctcca gcagcatcag ctctgcctat actgtcagcc
     4501 gccgctcctc cctggcctct cctttccccc ctggctcccc accagagaat ggagcatcct
     4561 ccctgcctgg ccttatgcct gcccagcact acctgcttcg ggcaagatat gcttcagcca
     4621 gagggggtgg tacttcgccc actgcagcat ccagcctgga tcggataggt ggtcttccca
     4681 tgcctccttg gagaagccga gccgagtatc caggatacaa ccccaatgca ggggtcaccc
     4741 ggagggccag tgacccagcc caggctgctg accgtcctgc tccagctaga gtccagaggt
     4801 tcaagagcct gggctgtgtc cataccccac ccactgtggc agggggagga cagaactttg
     4861 atccttacct cccaacctct gtctactcac cacagccccc cagcatcact gagaatgctg
     4921 ccatggatgc tagagggcta caggaagagc cagaagttgg gacctccatg gtgggcagtg
     4981 gtctgaaccc ctatatggac ttcccaccta ctgatactct gggatatggg ggacctgaag
     5041 gggcagcagc tgagccttat ggagcgaggg gtccaggctc tctgcctctt gggcctggtc
     5101 cacccaccaa ctatggcccc aacccctgtc cccagcaggc ctcatatcct gaccccaccc
     5161 aagaaacatg gggtgagttc ccttcccact ctgggctgta cccaggcccc aaggctctag
     5221 gtggaaccta cagccagtgt cctcgacttg aacattatgg acaagtgcaa gtcaagccag
     5281 aacaggggtg cccagtgggg tctgactcca caggactggc accctgcctc aatgcccacc
     5341 ccagtgaggg gcccccacat ccacagcctc tcttttccca ttacccccag ccctctcctc
     5401 cccaatatct ccagtcaggc ccctataccc agccaccccc tgattatctt ccttcagaac
     5461 ccaggccttg cctggacttt gattccccca cccattccac agggcagctc aaggctcagc
     5521 ttgtgtgtaa ttatgttcaa tctcaacagg agctactgtg ggagggtggg ggcagggaag
     5581 atgcccccgc ccaggaacct tcctaccaga gtcccaagtt tctggggggt tcccaggtta
     5641 gcccaagccg tgctaaagct ccagtgaaca catatggacc tggctttgga cccaacttgc
     5701 ccaatcacaa gtcaggttcc tatcccaccc cttcaccatg ccatgaaaat tttgtagtgg
     5761 gggcaaatag ggcttcacat agggcagcag caccacctcg acttctgccc ccattgccca
     5821 cttgctatgg gcctctcaaa gtgggaggca caaaccccag ctgtggtcat cctgaggtgg
     5881 gcaggctagg agggggtcct gccttgtacc ctcctcccga aggacaggta tgtaaccccc
     5941 tggactctct tgatcttgac aacactcagc tggactttgt ggctattctg gatgagcccc
     6001 aggggctgag tcctcctcct tcccatgatc agcggggcag ctctggacat accccacctc
     6061 cctctgggcc ccccaacatg gctgtgggca acatgagtgt cttactgaga tccctacctg
     6121 gggaaacaga attcctcaac tctagtgcct aaacccagct ttcttgtaca aagtggtgat
     6181 aatcgaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6601 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6781 cgcatcggga attcccgcgg ttcgaacgcg ttgacattga ttattgacta gttattaata
     6841 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     6901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     6961 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7021 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7081 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     7141 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     7201 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     7261 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     7321 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     7381 ctatataagc agagctctct ggctaactag agaacccact gcgccaccat ggtgagcaag
     7441 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     7501 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     7561 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     7621 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     7681 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     7741 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     7801 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     7861 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     7921 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     7981 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     8041 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     8101 cgccactcca ccggcggcat ggacgagctg tacaagggct ccggagaggg caggggaagt
     8161 cttctaacat gcggggacgt ggaggaaaat cccggcccca tggccaagcc tttgtctcaa
     8221 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     8281 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     8341 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     8401 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     8461 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     8521 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     8581 tatgtgtggg agggctaagg tacctttaag accaatgact tacaaggcag ctgtagatct
     8641 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     8701 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8881 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8941 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     9001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     9121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9241 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     9301 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     9361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     9421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     9481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     9961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    10321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    10621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    10681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    10741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    10801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    10861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    10921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    10981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    11101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    11161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    11221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    11281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    11341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    11401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    11461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagagagaaa
    11521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    11581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    11641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    11701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    11761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    11821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
    11881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
    11941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
    12001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    12061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
    12121 aagggaacaa aagctggagc tgcaagctt
//
