LOCUS       sequence-497425-        3552 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  263-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern 
            in the presence of ZFa and editable by Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Kabaria SR, Bae Y, Ehmann ME, Lende-Dorn BA, Beitz AM, Peterman EL, 
            Love KS, Ploessl DS, Galloway KE
  TITLE     Programmable promoter editing for precise control of transgene 
            expression.
  JOURNAL   Nat Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y.
  PUBMED    41083901
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Oct 13. doi: 10.1038/s41587-025-02854-y."
COMMENT     Sequence Label: (263-bp-DIAL-YB_TATA)-mGL-BGH_pKG2847
FEATURES             Location/Qualifiers
     source          1..3552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      83..92
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(89..91,92..94,95..808)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(134..155)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    820..1044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1155..1174)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1166..1975
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1233..1252)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2065..2653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2554..2573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      2818..2904
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2996..3023
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3031..3054
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    complement(3222..3255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3373..3397
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3452..3471
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(3519..3552)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggatttgat ctgatcaagc aactctagag ggtatataat gggggccact agtctactac
       61 cagagctcat cgctagcaat gagccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      121 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtccg
      181 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
      241 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttaggctacg gcgtggcctg
      301 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      361 aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca agacccgcgc
      421 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gtgctgaagg gcatcgactt
      481 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaaggt
      541 ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga cccgccacaa
      601 cgttgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
      661 cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccaaac tgagcaaaga
      721 ccccaacgag aagcgcgatc acatggtcct gaaggagagg gtgaccgccg ccgggattac
      781 acatgacatg gacgagctgt acaagtaaca acgttgcacc tgtgccttct agttgccagc
      841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
      901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
      961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1021 ctggggatgc ggtgggctct atggcgctcg ctgactgcag gtgcccgtgt ctcaaaatct
     1081 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1141 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     1201 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1261 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     1321 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     1381 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctggtgatgc
     1441 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     1501 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1561 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctagctc aggcgcaatc
     1621 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1681 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     1741 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1801 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1861 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1921 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1981 attggttaat tggttgtaac attattcaga ttgggccccg ttccactgag cgtcagaccc
     2041 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2101 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2161 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2221 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2281 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2341 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2401 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     2461 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     2521 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     2581 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     2641 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2701 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2761 tagcatggat ctcggggacg tctaactact aagcgagagt agggaactgc caggcatcaa
     2821 ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2881 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg
     2941 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag
     3001 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctgttag ttagttactt
     3061 aagctcgggc ccacctgcag tctacttact cggatcggga gatctgaatt ggagtgagga
     3121 cgtgtagagt gaggacgtgc ggagtgagga cgtgtagagt gaggacgtgc ggagtgagga
     3181 cgtgtagagt gaggacgtgc cctcctctgg atcaaatccg aataacttcg tatagcatac
     3241 attatacgaa gttatctcga gtcgctcggt acgatttaaa ttgaattctg cagtcgacgg
     3301 taccgcgggc ccgggatcct gatagtgacc tgttcgttgc aacaaattga taagcaatgc
     3361 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
     3421 gactggatcc ggtacctcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     3481 cattttggca aagaattgat taattcgagc gaacgcgtat aacttcgtat agcatacatt
     3541 atacgaagtt at
//
