LOCUS       sequence-497431-        5398 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  In vitro transcription (SP6 promotor) or mammalian expression (CMV 
            promotor) of the catalytic domain of zerbafish Adar1 (E488Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  TITLE     Cifuentes Lab Plasmids 2025
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2_N22_HA_dreAdar1_E488Q
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             97..162
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             163..189
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1450..1584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1474..1493)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1528..1547
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1697..1717)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1697..1715)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1736..1752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1736..1752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1749..1771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1760..1776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1784..1790,1791..1808,1809..1814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1829..1850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1967..1984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2138..2726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2218..2237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2897..3688,3689..3757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3520..3539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3758..3862)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3888..4343)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4025..4046)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4237..4256
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4414..5398
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgccatgg acgcgcagac gcggcgacgc
      121 gagcgccggg cagagaagca ggctcaatgg aaggcagcta actacccata cgatgttcca
      181 gattacgctg gcggacagct ggaccaaaca ccgtcccgac agccaatccc cagggaggga
      241 cttcagctgc atttgcccca ggtcctcgcg gatgcggtct ctcgtttagt tgtggataag
      301 ttcagcgagc tgacagacaa cttctcgtct ccacatgcac ggcggaaagt cctggcagga
      361 gttgtcatga caaccggtaa ggatgtgatg gacgctcagg tgatttgtgt gacgacggga
      421 accaagtgta taaacggaga gtacatgagc gatcgaggac tggcccttaa tgactgccat
      481 gctgagatca tcgcacggcg ctcactcatt agatacctgt acaatcagct ggaatacttc
      541 ctcagcgaca gcactgaaga acatgagaaa tccatattca gatggtgcag tgaacatggc
      601 taccggctaa aagatgatat tcaattccat ctgtatatca gcacgtcgcc ctgtggagac
      661 gcccggatct tctcccctca tgaggctggt gccgaggacc agggtgaccg acatccaaac
      721 cgcaaggccc gtggacaact acgaaccaag attgagtctg gacaaggaac gatacctgtt
      781 cgtacgagta acaccattca gacttgggat ggagtcctgc agggagagcg actgctaacc
      841 atgtcatgca gtgacaaaat tgccagatgg aacgtgatcg gagtacaggg atctcttctg
      901 agttacttca cagagcccat ctatttctcc agtttaattc tgggaagtct gtatcatgct
      961 gaccatcttt ccagagccat gtaccagcgc atcgcagaca tggatgatat tcccccgcca
     1021 ttcagaatca acagaccact tcttagcggt atcagtaaca cagaagcaag gcaacctggg
     1081 aaagctccta atttcagcgt caactggaca gtaggagacc aggggttgga gatcattaac
     1141 gcaaccacag gaaaagatga cctcggaaga cagtcgcaac tttgcaaaca cgctctttac
     1201 agtcgttggg tctgtctaca tgcaaagctc agtgagactc tgcggatccg aggatctcgg
     1261 cctggctcct accacgaggc aaaacaaggg gcggtcgagt atcatgaggc gaaacagact
     1321 cttttcaaag ctttctacaa agcgggactg ggagcatggg tggaaaaacc catcgaacaa
     1381 gaccagtttt cccttactcc ctagcaaggc ctctcgagcc tctagaacta tagtgagtcg
     1441 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     1501 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     1561 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     1621 cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg
     1681 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     1741 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga
     1801 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1861 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1921 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1981 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2041 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2101 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2161 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2221 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2281 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2341 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2401 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2461 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     2521 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2581 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2641 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2701 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2761 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2821 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2881 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2941 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     3001 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     3061 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     3121 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     3181 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3241 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3301 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3361 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3421 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3481 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3541 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3601 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3661 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3721 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3781 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3841 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     3901 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3961 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4021 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4081 aaaaatcgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4141 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4201 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4261 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4321 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     4381 gcgatcggtg cgggcctctt cgctattacg ccagtcgacc gccaattcaa tatggcgtat
     4441 atggactcat gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat
     4501 atggactcgt gccaattcaa tatggtggat ctggacccca gccaattcaa tatggcggac
     4561 ttggcaccat gccaattcaa tatggcggac ctggcactgt gccaactggg gaggggtcta
     4621 cttggcacgg tgccaagttt gaggaggggt cttggccctg tgccaagtcc gccatattga
     4681 attggcatgg tgccaataat ggcggccata ttggctatat gccaggatca atatataggc
     4741 aatatccaat atggccctat gccaatatgg ctattggcca ggttcaatac tatgtattgg
     4801 ccctatgcca tatagtattc catatatggg ttttcctatt gacgtagata gcccctccca
     4861 atgggcggtc ccatatacca tatatggggc ttcctaatac cgcccatagc cactccccca
     4921 ttgacgtcaa tggtctctat atatggtctt tcctattgac gtcatatggg cggtcctatt
     4981 gacgtatatg gcgcctcccc cattgacgtc aattacggta aatggcccgc ctggctcaat
     5041 gcccattgac gtcaatagga ccacccacca ttgacgtcaa tgggatggct cattgcccat
     5101 tcatatccgt tctcacgccc cctattgacg tcaatgacgg taaatggccc acttggcagt
     5161 acatcaatat ctattaatag taacttggca agtacattac tattggaagt acgccagggt
     5221 acattggcag tactcccatt gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt
     5281 acatccccat tgacgtcaat ggggaggggc aatgacgcaa atgggcgttc cattgacgta
     5341 aatgggcggt aggcgtgcct aatgggaggt ctatataagc aatgctcgtt tagggaac
//
