LOCUS       sequence-497439-       10559 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box))..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2724 - pLenti-X1-EF1A-HA-FBXO31(∆F-box)-PGK...
FEATURES             Location/Qualifiers
     source          1..10559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3009..3033
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3164..4342
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3395..4333
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4290..4310
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4359..4377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4425..4451
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5981..6005)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6036..6624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6089..6109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6507..6518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6675..7174
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6710..6733)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7107..7126
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7188..7991
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7251..7270)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7861..7880
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8135..8368
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8440..8561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8477..8496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8531..8550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8601..8736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8757..8775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8785..8802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8785..8801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8794..8816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8943..9398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9030..9049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9240..9261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9424..9528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9529..9597,9598..10389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9747..9766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggaattctgc
     3001 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccgac attgattatt
     3061 gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct
     3121 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     3181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     3361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     3421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     3481 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3541 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3601 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3661 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3721 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3781 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3841 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3901 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3961 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     4021 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     4081 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     4141 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     4201 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     4261 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     4321 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     4381 acccaagctg gctaggtaag cttggtaccg agctcggatc catgtaccca tacgatgttc
     4441 cagattacgc tggtggatct ggaggttctg gtggaagcgc tatggcggtg tgtgctcgcc
     4501 tttgcggcgt gggcccgtcg cgcggatgtc ggcgccgcca gcagcgccgg ggcccggccg
     4561 agacggcggc ggccgacagc gagccggaca cagaccccga ggaggagcgc atcgaggcta
     4621 gcgccggggt cgggggcggc ttgtgcgcgg gcccctcgcc gccgcccccg cgctgctcgc
     4681 tgctggagct gccggagtat ggtgtttgcg aaaacttgcg gaagctggag atcacaggcg
     4741 tgtcttgtcg ggacgtctat gcgaagctgc ttcaccgata tagacacatt ttgggattgt
     4801 ggcagccaga tatcgggcca tacggaggac tgctgaacgt ggtggtggac ggcctgttca
     4861 tcatcgggtg gatgtacctg cctccccatg acccccacgt cgatgaccct atgagattca
     4921 agcctctgtt caggatccac ctgatggaga ggaaggctgc cacagtggag tgcatgtacg
     4981 gccacaaagg gccccaccac ggccacatcc agattgtgaa gaaggatgag ttctccacca
     5041 agtgcaacca gacggaccac cacaggatgt ccggcgggag gcaggaggag tttcggacgt
     5101 ggctgaggga ggaatggggg cgcacgctgg aggacatctt ccacgagcac atgcaggagc
     5161 tcatcctgat gaagttcatc tacaccagtc agtacgacaa ctgcctgacc taccgccgca
     5221 tctacctgcc gcccagccgc cccgacgacc tcatcaagcc tggcctcttc aaaggtacct
     5281 atggcagcca cggcctggag attgtgatgc tcagcttcca cggccggcgt gccaggggca
     5341 ccaagatcac gggcgacccc aacatccccg ctgggcagca gacagtggag atcgacctga
     5401 ggcatcggat ccagctgccc gacctcgaga accagcgcaa cttcaatgag ctctcccgca
     5461 tcgtcctgga ggtgcgcgag agggtgcgcc aggagcagca ggaaggcggg cacgaggcgg
     5521 gcgagggtcg tggccggcag ggcccccggg agtcccagcc aagccctgcc cagcccaggg
     5581 cagaggcgcc cagcaagggc ccagatggga cacctggtga ggatggtggc gagcctgggg
     5641 atgccgtagc tgcggccgag cagcctgccc agtgtgggca ggggcagccg ttcgtgctgc
     5701 ccgtgggcgt gagctccagg aatgaggact acccccgaac ctgcaggatg tgtttttatg
     5761 gcacaggcct catcgcgggc cacggcttca ccagccctga acgcaccccc ggggtcttca
     5821 tcctcttcga tgaggaccgc ttcgggttcg tctggctgga gctgaaatcc ttcagcctgt
     5881 acagccgggt ccaggccacc ttccggaacg cagatgcgcc gtccccacag gccttcgatg
     5941 agatgctcaa gaacattcag tccctcacct cctgatctag acccagcttt cttgtacaaa
     6001 gtggttgata tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt
     6061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6421 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6601 tctccctttg ggccgcctcc ccgcctggac ctgcaggatt ctgcagatat ccggactagt
     6661 gatctaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     6721 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     6781 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     6841 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     6901 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     6961 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     7021 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     7081 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     7141 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agccaatatg ggatcggcca
     7201 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7261 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7321 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7381 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7441 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7501 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7561 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7621 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7681 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7741 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7801 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7861 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7921 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7981 agttcttctg aacgcgtcgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac
     8041 gaccccatgc atctcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta
     8101 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga
     8161 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     8221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8341 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     8401 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     8461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8581 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8641 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8701 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8761 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8821 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8881 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8941 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9001 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9061 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9121 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     9181 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9241 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9301 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9361 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     9421 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9481 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9541 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9601 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9661 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9721 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9781 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9841 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9901 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9961 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    10021 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    10081 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    10141 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    10201 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    10261 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10321 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10381 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10441 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10501 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
