LOCUS       sequence-497446-       12752 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box))..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2732 - pHR-EF1A-HA-FBXO31(∆F-box)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..12752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5271..5387
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5421..5445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5576..6754
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5807..6745
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6702..6722
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6771..6790
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6771..6789
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6837..6863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8393..8417)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8462..8486)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8518..9084
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8534..9086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8678..8695)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8905..8924
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9098..9100,9101..9103,9104..9817)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9143..9164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9404..9423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9751..9772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9855..10443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9908..9928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10326..10337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10446..10462)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10447..10463)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10553..10786
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10811..10829)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10812..10829)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11119..11138)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11352..11371)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11471..11493
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11531..11549)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11617..11721
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11722..11790,11791..12582)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11940..11959)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     5281 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     5341 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gtccagtgtg
     5401 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccacc atgggaaccg
     5461 acattgatta ttgactaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac
     5521 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     5581 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5641 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5701 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5761 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     5821 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     5881 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5941 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     6001 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     6061 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     6121 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6181 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6241 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6301 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6361 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6421 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6481 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6541 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6601 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6661 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6721 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact
     6781 cactataggg agacccaagc tggctaggta agcttggtac cgagctcgga tccatgtacc
     6841 catacgatgt tccagattac gctggtggat ctggaggttc tggtggaagc gctatggcgg
     6901 tgtgtgctcg cctttgcggc gtgggcccgt cgcgcggatg tcggcgccgc cagcagcgcc
     6961 ggggcccggc cgagacggcg gcggccgaca gcgagccgga cacagacccc gaggaggagc
     7021 gcatcgaggc tagcgccggg gtcgggggcg gcttgtgcgc gggcccctcg ccgccgcccc
     7081 cgcgctgctc gctgctggag ctgccggagt atggtgtttg cgaaaacttg cggaagctgg
     7141 agatcacagg cgtgtcttgt cgggacgtct atgcgaagct gcttcaccga tatagacaca
     7201 ttttgggatt gtggcagcca gatatcgggc catacggagg actgctgaac gtggtggtgg
     7261 acggcctgtt catcatcggg tggatgtacc tgcctcccca tgacccccac gtcgatgacc
     7321 ctatgagatt caagcctctg ttcaggatcc acctgatgga gaggaaggct gccacagtgg
     7381 agtgcatgta cggccacaaa gggccccacc acggccacat ccagattgtg aagaaggatg
     7441 agttctccac caagtgcaac cagacggacc accacaggat gtccggcggg aggcaggagg
     7501 agtttcggac gtggctgagg gaggaatggg ggcgcacgct ggaggacatc ttccacgagc
     7561 acatgcagga gctcatcctg atgaagttca tctacaccag tcagtacgac aactgcctga
     7621 cctaccgccg catctacctg ccgcccagcc gccccgacga cctcatcaag cctggcctct
     7681 tcaaaggtac ctatggcagc cacggcctgg agattgtgat gctcagcttc cacggccggc
     7741 gtgccagggg caccaagatc acgggcgacc ccaacatccc cgctgggcag cagacagtgg
     7801 agatcgacct gaggcatcgg atccagctgc ccgacctcga gaaccagcgc aacttcaatg
     7861 agctctcccg catcgtcctg gaggtgcgcg agagggtgcg ccaggagcag caggaaggcg
     7921 ggcacgaggc gggcgagggt cgtggccggc agggcccccg ggagtcccag ccaagccctg
     7981 cccagcccag ggcagaggcg cccagcaagg gcccagatgg gacacctggt gaggatggtg
     8041 gcgagcctgg ggatgccgta gctgcggccg agcagcctgc ccagtgtggg caggggcagc
     8101 cgttcgtgct gcccgtgggc gtgagctcca ggaatgagga ctacccccga acctgcagga
     8161 tgtgttttta tggcacaggc ctcatcgcgg gccacggctt caccagccct gaacgcaccc
     8221 ccggggtctt catcctcttc gatgaggacc gcttcgggtt cgtctggctg gagctgaaat
     8281 ccttcagcct gtacagccgg gtccaggcca ccttccggaa cgcagatgcg ccgtccccac
     8341 aggccttcga tgagatgctc aagaacattc agtccctcac ctcctgatct agacccagct
     8401 ttcttgtaca aagtggttga tatccagcac agtggcggcc gccccgggtt aattaatcta
     8461 gacccagctt tcttgtacaa agtggtgacg acggtatcga taagcttgat atcgaattcc
     8521 gccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     8581 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     8641 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     8701 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     8761 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     8821 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     8881 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     8941 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     9001 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     9061 ttcctttgaa aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct
     9121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     9181 cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     9241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     9301 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     9361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     9421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     9481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     9541 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga acttcaagat
     9601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     9661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     9721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     9781 cgggatcact ctcggcatgg acgagctgta caagtaacct gcaggcatgc aagcttgata
     9841 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     9901 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     9961 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
    10021 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
    10081 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
    10141 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
    10201 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
    10261 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
    10321 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
    10381 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
    10441 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
    10501 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
    10561 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
    10621 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10681 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10741 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
    10801 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
    10861 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
    10921 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
    10981 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
    11041 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
    11101 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
    11161 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
    11221 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
    11281 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
    11341 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
    11401 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
    11461 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
    11521 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
    11581 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    11641 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    11701 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    11761 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    11821 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    11881 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    11941 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    12001 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    12061 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    12121 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    12181 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    12241 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    12301 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    12361 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    12421 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    12481 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    12541 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    12601 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    12661 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    12721 tcagaccccg tagaaaagat caaaggatct tc
//
