LOCUS       sequence-497447-       11069 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  Inducible expression of FBXO31 with the shield degron system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2737 - pHR-EF1A-DD-3xFLAG-FBXO31-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..11069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3009..3033
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3164..4342
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3395..4333
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4290..4310
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4359..4377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4422..4431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4428..4430,4431..4751)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             4770..4835
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(6491..6515)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6546..7134
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6599..6619)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7017..7028)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7185..7684
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7220..7243)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7617..7636
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7698..8501
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7761..7780)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8371..8390
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8645..8878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8950..9071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8987..9006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9041..9060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9111..9246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9267..9285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9295..9312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9295..9311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9304..9326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9453..9908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9540..9559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9750..9771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9934..10038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10039..10107,10108..10899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10257..10276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggaattctgc
     3001 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccgac attgattatt
     3061 gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct
     3121 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     3181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     3361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     3421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     3481 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3541 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3601 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3661 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3721 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3781 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3841 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3901 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3961 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     4021 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     4081 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     4141 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     4201 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     4261 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     4321 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     4381 acccaagctg gctaggtaag cttggtaccg agctcggatc cgccaccatg ggagtgcagg
     4441 tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag acctgtgtgg
     4501 tgcactacac cgggatgctt gaagatggaa agaaagtcga ttcctcccgg gacagaaaca
     4561 agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa gaaggggttg
     4621 cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat gcctatggtg
     4681 ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat gtggagcttc
     4741 taaaaccgga agctagcgct accggaatgg actacaaaga ccatgacggt gattataaag
     4801 atcatgacat cgattacaag gatgacgatg acaagggagg ttctggtgga tctggtggaa
     4861 gcgctatggc ggtgtgtgct cgcctttgcg gcgtgggccc gtcgcgcgga tgtcggcgcc
     4921 gccagcagcg ccggggcccg gccgagacgg cggcggccga cagcgagccg gacacagacc
     4981 ccgaggagga gcgcatcgag gctagcgccg gggtcggggg cggcttgtgc gcgggcccct
     5041 cgccgccgcc cccgcgctgc tcgctgctgg agctgccgcc cgagctgctg gtggagatct
     5101 tcgcgtcgct gccgggcacg gacctaccca gcttggccca ggtctgcacg aagttccggc
     5161 gcatcctcca caccgacacc atctggagga ggcgttgccg tgaggagtat ggtgtttgcg
     5221 aaaacttgcg gaagctggag atcacaggcg tgtcttgtcg ggacgtctat gcgaagctgc
     5281 ttcaccgata tagacacatt ttgggattgt ggcagccaga tatcgggcca tacggaggac
     5341 tgctgaacgt ggtggtggac ggcctgttca tcatcgggtg gatgtacctg cctccccatg
     5401 acccccacgt cgatgaccct atgagattca agcctctgtt caggatccac ctgatggaga
     5461 ggaaggctgc cacagtggag tgcatgtacg gccacaaagg gccccaccac ggccacatcc
     5521 agattgtgaa gaaggatgag ttctccacca agtgcaacca gacggaccac cacaggatgt
     5581 ccggcgggag gcaggaggag tttcggacgt ggctgaggga ggaatggggg cgcacgctgg
     5641 aggacatctt ccacgagcac atgcaggagc tcatcctgat gaagttcatc tacaccagtc
     5701 agtacgacaa ctgcctgacc taccgccgca tctacctgcc gcccagccgc cccgacgacc
     5761 tcatcaagcc tggcctcttc aaaggtacct atggcagcca cggcctggag attgtgatgc
     5821 tcagcttcca cggccggcgt gccaggggca ccaagatcac gggcgacccc aacatccccg
     5881 ctgggcagca gacagtggag atcgacctga ggcatcggat ccagctgccc gacctcgaga
     5941 accagcgcaa cttcaatgag ctctcccgca tcgtcctgga ggtgcgcgag agggtgcgcc
     6001 aggagcagca ggaaggcggg cacgaggcgg gcgagggtcg tggccggcag ggcccccggg
     6061 agtcccagcc aagccctgcc cagcccaggg cagaggcgcc cagcaagggc ccagatggga
     6121 cacctggtga ggatggtggc gagcctgggg atgccgtagc tgcggccgag cagcctgccc
     6181 agtgtgggca ggggcagccg ttcgtgctgc ccgtgggcgt gagctccagg aatgaggact
     6241 acccccgaac ctgcaggatg tgtttttatg gcacaggcct catcgcgggc cacggcttca
     6301 ccagccctga acgcaccccc ggggtcttca tcctcttcga tgaggaccgc ttcgggttcg
     6361 tctggctgga gctgaaatcc ttcagcctgt acagccgggt ccaggccacc ttccggaacg
     6421 cagatgcgcc gtccccacag gccttcgatg agatgctcaa gaacattcag tccctcacct
     6481 cctgatctag acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     6541 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6601 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6661 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6721 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6781 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6841 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6901 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6961 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7021 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7081 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggac
     7141 ctgcaggatt ctgcagatat ccggactagt gatctaattc taccgggtag gggaggcgct
     7201 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
     7261 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
     7321 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
     7381 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
     7441 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
     7501 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg
     7561 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
     7621 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
     7681 ttcgacctgc agccaatatg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
     7741 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7801 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7861 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     7921 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7981 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     8041 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8101 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8161 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8221 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8281 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8341 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8401 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8461 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aacgcgtcgc ccgccccacg
     8521 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
     8581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8641 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8821 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8881 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8941 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9061 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     9121 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9181 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9241 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     9301 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     9361 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9421 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9901 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9961 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10021 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10081 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10141 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10201 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10261 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    10321 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10381 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10441 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10501 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10561 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10621 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10681 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10741 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10801 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10861 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10921 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10981 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11041 gaccccgtag aaaagatcaa aggatcttc
//
