LOCUS       sequence-497451-       12878 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N))..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12878)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 12878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2734 - pHR-EF1A-HA-FBXO31(D334N)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..12878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(830..850)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2975..2994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3029..3048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5271..5387
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    5421..5445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5576..6754
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5807..6745
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6702..6722
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6771..6790
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6771..6789
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6837..6863
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8519..8543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8588..8612)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8644..9210
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8660..9212
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8804..8821)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9031..9050
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9224..9226,9227..9229,9230..9943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9269..9290)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9530..9549)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9877..9898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9981..10569
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10034..10054)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10452..10463)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10572..10588)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10573..10589)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10679..10912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10937..10955)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10938..10955)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11245..11264)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11478..11497)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11597..11619
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11657..11675)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11743..11847
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11848..11916,11917..12708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12066..12085)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga
     5281 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     5341 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gtccagtgtg
     5401 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctccacc atgggaaccg
     5461 acattgatta ttgactaggc ttttgcaaaa agctttgcaa agatggataa agttttaaac
     5521 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     5581 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5641 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5701 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5761 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     5821 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     5881 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5941 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     6001 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     6061 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     6121 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6181 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6241 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6301 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6361 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6421 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6481 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6541 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6601 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6661 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6721 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact
     6781 cactataggg agacccaagc tggctaggta agcttggtac cgagctcgga tccatgtacc
     6841 catacgatgt tccagattac gctggtggat ctggaggttc tggtggaagc gctatggcgg
     6901 tgtgtgctcg cctttgcggc gtgggcccgt cgcgcggatg tcggcgccgc cagcagcgcc
     6961 ggggcccggc cgagacggcg gcggccgaca gcgagccgga cacagacccc gaggaggagc
     7021 gcatcgaggc tagcgccggg gtcgggggcg gcttgtgcgc gggcccctcg ccgccgcccc
     7081 cgcgctgctc gctgctggag ctgccgcccg agctgctggt ggagatcttc gcgtcgctgc
     7141 cgggcacgga cctacccagc ttggcccagg tctgcacgaa gttccggcgc atcctccaca
     7201 ccgacaccat ctggaggagg cgttgccgtg aggagtatgg tgtttgcgaa aacttgcgga
     7261 agctggagat cacaggcgtg tcttgtcggg acgtctatgc gaagctgctt caccgatata
     7321 gacacatttt gggattgtgg cagccagata tcgggccata cggaggactg ctgaacgtgg
     7381 tggtggacgg cctgttcatc atcgggtgga tgtacctgcc tccccatgac ccccacgtcg
     7441 atgaccctat gagattcaag cctctgttca ggatccacct gatggagagg aaggctgcca
     7501 cagtggagtg catgtacggc cacaaagggc cccaccacgg ccacatccag attgtgaaga
     7561 aggatgagtt ctccaccaag tgcaaccaga cggaccacca caggatgtcc ggcgggaggc
     7621 aggaggagtt tcggacgtgg ctgagggagg aatgggggcg cacgctggag gacatcttcc
     7681 acgagcacat gcaggagctc atcctgatga agttcatcta caccagtcag tacgacaact
     7741 gcctgaccta ccgccgcatc tacctgccgc ccagccgccc cgacgacctc atcaagcctg
     7801 gcctcttcaa aggtacctat ggcagccacg gcctggagat tgtgatgctc agcttccacg
     7861 gccggcgtgc caggggcacc aagatcacgg gcaaccccaa catccccgct gggcagcaga
     7921 cagtggagat cgacctgagg catcggatcc agctgcccga cctcgagaac cagcgcaact
     7981 tcaatgagct ctcccgcatc gtcctggagg tgcgcgagag ggtgcgccag gagcagcagg
     8041 aaggcgggca cgaggcgggc gagggtcgtg gccggcaggg cccccgggag tcccagccaa
     8101 gccctgccca gcccagggca gaggcgccca gcaagggccc agatgggaca cctggtgagg
     8161 atggtggcga gcctggggat gccgtagctg cggccgagca gcctgcccag tgtgggcagg
     8221 ggcagccgtt cgtgctgccc gtgggcgtga gctccaggaa tgaggactac ccccgaacct
     8281 gcaggatgtg tttttatggc acaggcctca tcgcgggcca cggcttcacc agccctgaac
     8341 gcacccccgg ggtcttcatc ctcttcgatg aggaccgctt cgggttcgtc tggctggagc
     8401 tgaaatcctt cagcctgtac agccgggtcc aggccacctt ccggaacgca gatgcgccgt
     8461 ccccacaggc cttcgatgag atgctcaaga acattcagtc cctcacctcc tgatctagac
     8521 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgccc cgggttaatt
     8581 aatctagacc cagctttctt gtacaaagtg gtgacgacgg tatcgataag cttgatatcg
     8641 aattccgccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     8701 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     8761 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     8821 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     8881 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     8941 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     9001 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     9061 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     9121 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     9181 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga
     9241 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     9301 caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     9361 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     9421 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     9481 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     9541 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     9601 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     9661 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt
     9721 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     9781 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     9841 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     9901 cgccgccggg atcactctcg gcatggacga gctgtacaag taacctgcag gcatgcaagc
     9961 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
    10021 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    10081 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    10141 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    10201 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    10261 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    10321 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    10381 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    10441 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
    10501 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
    10561 cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
    10621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
    10681 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
    10741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
    10921 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
    10981 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
    11041 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
    11101 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
    11161 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
    11221 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
    11281 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
    11341 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
    11401 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
    11461 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
    11521 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    11581 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    11641 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
    11701 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    11761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    11821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    11881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    11941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    12001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    12061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    12121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    12181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    12241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    12301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    12361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    12421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    12481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    12541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    12601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    12661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    12721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    12781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    12841 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
