LOCUS       sequence-497462-       10910 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  For overexpression of RNAPII.CTD-YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  TITLE     Hnisz lab plasmids 2025
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_RNAPII_CTD_YFP
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             join(1920..1922,1923..1925,1926..2636)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2226..2245)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2573..2594
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2646..2666
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2767..3355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2820..2840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3238..3249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3358..3374)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3359..3375)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3465..3698
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3723..3741)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3724..3741)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4031..4050)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4264..4283)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4383..4405
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4443..4461)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4529..4633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4634..4702,4703..5494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGTLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4852..4871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5665..6253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6154..6173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6407..6424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6494..6514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6499..6828
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6679..6814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7962..8027
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8157..8177
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8602..8736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8639..8658)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8693..8712
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8904..9537
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9584..9709
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10206..10439
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10624..10668
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccatgtac tcgccaacgt cacctgccta cgagccccgc tctcctgggg
      841 gctacacacc ccagagtccc tcttattccc ccacttcacc ctcctactcc cctacctctc
      901 catcctattc tccaaccagt cccaactata gtcccacatc acccagctat tcgccaacgt
      961 cacccagcta ctcaccgacc tctcccagct actcacccac ctctcccagc tactcgccca
     1021 cctctcccag ctactcgccc acctctccca gctactcacc cacttcccct agctactcgc
     1081 ccacttcccc tagctactcg ccaacgtctc ccagctactc gccgacatct cccagctact
     1141 cgccaacttc acccagctat tctcccactt ctcccagcta ctcacctacc tctccaagct
     1201 attcacccac ctcccccagc tactcaccca cttccccaag ttactcaccc accagcccga
     1261 actattctcc aaccagtccc aattacaccc caacatcacc cagctacagc ccgacatcac
     1321 ccagctattc acctactagt cccaactaca cacctaccag ccctaactac agcccaacct
     1381 ctccaagcta ctctccaaca tcacccagct attccccgac ctcaccaagt tactcccctt
     1441 ccagcccacg atacacacca cagtctccaa cctatacccc aagctcaccc agctacagcc
     1501 ccagctcgcc cagctacagc ccaacctcac ccaagtacac cccaaccagt ccttcttaca
     1561 gtcccagctc cccagagtat accccaacct ctcccaagta ctcacctacc agtcccaaat
     1621 attcacccac ctctcccaag tactcgccta ccagtcccac ctattcaccc accaccccaa
     1681 aatactcccc aacatctcct acttattccc caacctctcc agtctacacc ccaacctctc
     1741 ccaagtactc acctactagc cccacttact cgcccacttc ccccaagtac tcgcccacca
     1801 gccccaccta ctcgcccacc tcccccaaag gctcaaccta ctctcccact tcccctggtt
     1861 actcgcccac cagccccacc tacagtctca caagcccggc tggaggtagc ggaggacata
     1921 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1981 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2041 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2101 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
     2161 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2221 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2281 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2341 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2401 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2461 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2521 acctgagcta ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2581 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggag
     2641 gcggtccaaa aaagaagaga aaggtatgag cggccgcaac tcccacctgc aacatgcgtg
     2701 actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt
     2761 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2821 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2881 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2941 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3001 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3061 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3121 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3181 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3241 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3301 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3361 taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     3421 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     3481 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     3541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     3601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     3661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     3721 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     3781 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     3841 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     3901 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     3961 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     4021 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     4081 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     4141 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     4201 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     4261 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4321 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4381 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4441 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4501 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5281 aacacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6361 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6421 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6481 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     6541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     6841 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     6901 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     6961 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     7021 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     7081 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     7141 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     7201 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     7261 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     7321 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     7381 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     7441 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     7501 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     7561 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     7621 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     7681 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     7741 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     7801 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     7861 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
     7921 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     7981 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     8041 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     8101 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     8161 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     8221 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     8281 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     8341 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     8401 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     8461 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     8521 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     8581 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     8641 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8701 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     8761 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     8821 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     8881 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     8941 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     9001 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     9061 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     9121 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     9181 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     9241 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     9301 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     9361 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9421 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9481 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     9541 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     9601 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     9661 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     9721 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     9781 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     9841 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     9901 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     9961 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
    10021 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
    10081 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    10141 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    10201 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    10261 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    10321 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10381 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10441 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10501 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10561 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10621 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10681 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10741 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    10801 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    10861 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
//
