LOCUS       sequence-497479-       10685 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(D334N))..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10685)
  AUTHORS   Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, 
            Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters 
            Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek 
            M, Schulman BA, Bode JW, Corn JE
  TITLE     C-terminal amides mark proteins for degradation via SCF-FBXO31.
  JOURNAL   Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.
  PUBMED    39880951
REFERENCE   2  (bases 1 to 10685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2025-02"; volume: "638"; issue: "8050"; pages: 
            "519-527"
COMMENT     Sequence Label: pJC2728 - pLenti-X1-EF1A-HA-FBXO31(D334N)-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..10685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3009..3033
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3164..4342
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3395..4333
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4290..4310
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4359..4377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4425..4451
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6107..6131)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6162..6750
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6215..6235)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6633..6644)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6801..7300
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6836..6859)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7233..7252
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7314..8117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7377..7396)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7987..8006
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8261..8494
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8566..8687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8603..8622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8657..8676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8727..8862
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8883..8901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8911..8928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8911..8927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8920..8942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9069..9524
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9156..9175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9366..9387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9550..9654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9655..9723,9724..10515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9873..9892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcgt ccagtgtggt ggaattctgc
     3001 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccgac attgattatt
     3061 gactaggctt ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct
     3121 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     3181 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     3241 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     3301 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     3361 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     3421 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     3481 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3541 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3601 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3661 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3721 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3781 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3841 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     3901 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3961 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     4021 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     4081 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     4141 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     4201 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     4261 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     4321 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     4381 acccaagctg gctaggtaag cttggtaccg agctcggatc catgtaccca tacgatgttc
     4441 cagattacgc tggtggatct ggaggttctg gtggaagcgc tatggcggtg tgtgctcgcc
     4501 tttgcggcgt gggcccgtcg cgcggatgtc ggcgccgcca gcagcgccgg ggcccggccg
     4561 agacggcggc ggccgacagc gagccggaca cagaccccga ggaggagcgc atcgaggcta
     4621 gcgccggggt cgggggcggc ttgtgcgcgg gcccctcgcc gccgcccccg cgctgctcgc
     4681 tgctggagct gccgcccgag ctgctggtgg agatcttcgc gtcgctgccg ggcacggacc
     4741 tacccagctt ggcccaggtc tgcacgaagt tccggcgcat cctccacacc gacaccatct
     4801 ggaggaggcg ttgccgtgag gagtatggtg tttgcgaaaa cttgcggaag ctggagatca
     4861 caggcgtgtc ttgtcgggac gtctatgcga agctgcttca ccgatataga cacattttgg
     4921 gattgtggca gccagatatc gggccatacg gaggactgct gaacgtggtg gtggacggcc
     4981 tgttcatcat cgggtggatg tacctgcctc cccatgaccc ccacgtcgat gaccctatga
     5041 gattcaagcc tctgttcagg atccacctga tggagaggaa ggctgccaca gtggagtgca
     5101 tgtacggcca caaagggccc caccacggcc acatccagat tgtgaagaag gatgagttct
     5161 ccaccaagtg caaccagacg gaccaccaca ggatgtccgg cgggaggcag gaggagtttc
     5221 ggacgtggct gagggaggaa tgggggcgca cgctggagga catcttccac gagcacatgc
     5281 aggagctcat cctgatgaag ttcatctaca ccagtcagta cgacaactgc ctgacctacc
     5341 gccgcatcta cctgccgccc agccgccccg acgacctcat caagcctggc ctcttcaaag
     5401 gtacctatgg cagccacggc ctggagattg tgatgctcag cttccacggc cggcgtgcca
     5461 ggggcaccaa gatcacgggc aaccccaaca tccccgctgg gcagcagaca gtggagatcg
     5521 acctgaggca tcggatccag ctgcccgacc tcgagaacca gcgcaacttc aatgagctct
     5581 cccgcatcgt cctggaggtg cgcgagaggg tgcgccagga gcagcaggaa ggcgggcacg
     5641 aggcgggcga gggtcgtggc cggcagggcc cccgggagtc ccagccaagc cctgcccagc
     5701 ccagggcaga ggcgcccagc aagggcccag atgggacacc tggtgaggat ggtggcgagc
     5761 ctggggatgc cgtagctgcg gccgagcagc ctgcccagtg tgggcagggg cagccgttcg
     5821 tgctgcccgt gggcgtgagc tccaggaatg aggactaccc ccgaacctgc aggatgtgtt
     5881 tttatggcac aggcctcatc gcgggccacg gcttcaccag ccctgaacgc acccccgggg
     5941 tcttcatcct cttcgatgag gaccgcttcg ggttcgtctg gctggagctg aaatccttca
     6001 gcctgtacag ccgggtccag gccaccttcc ggaacgcaga tgcgccgtcc ccacaggcct
     6061 tcgatgagat gctcaagaac attcagtccc tcacctcctg atctagaccc agctttcttg
     6121 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     6181 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6241 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6301 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     6361 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     6421 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     6481 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6541 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6601 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6661 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     6721 gtcggatctc cctttgggcc gcctccccgc ctggacctgc aggattctgc agatatccgg
     6781 actagtgatc taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     6841 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     6901 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     6961 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     7021 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     7081 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     7141 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     7201 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     7261 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc aatatgggat
     7321 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     7381 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     7441 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     7501 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     7561 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     7621 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     7681 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     7741 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     7801 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     7861 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     7921 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     7981 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     8041 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     8101 ttgacgagtt cttctgaacg cgtcgcccgc cccacgaccc gcagcgcccg accgaaagga
     8161 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     8221 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     8281 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8341 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8401 tgagtgcttc aagtagtgta tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8461 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8521 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8581 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8641 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8701 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8761 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8821 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8881 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9061 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9481 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9541 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9601 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9661 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9721 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9781 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9841 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     9901 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     9961 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10021 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10081 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10141 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10201 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10261 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10321 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10381 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10441 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10501 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10561 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10621 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10681 tcttc
//
