LOCUS       sequence-497523-        3591 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41373
FEATURES             Location/Qualifiers
     source          1..3591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      886..1474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1375..1394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1628..1645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2715..3060
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3003..3023
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(3103..3121)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3189..3293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3515..3591,1..715)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
       61 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
      121 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
      181 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
      241 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
      301 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
      361 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
      421 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
      481 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
      541 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
      601 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
      661 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
      721 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
      781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
      841 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1441 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1501 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1561 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1621 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     1681 ccccgcgcgt tggccgattc attaatcact ctgtggtctc aggaggaatt ccaatcccac
     1741 aaaaatctga gcttaacagc acagttgctc ctctcagagc agaatcgggt attcaacacc
     1801 ctcatatcaa ctactacgtt gtgtataacg gtccacatgc cggtatatac gatgactggg
     1861 gttgtacaaa ggcggcaaca aacggcgttc ccggagttgc acacaagaaa tttgccacta
     1921 ttacagaggc aagagcagca gctgacgcgt acacaacaag tcagcaaaca gacaggttga
     1981 acttcatccc caaaggagaa gctcaactca agcccaagag ctttgctaag gccctaacaa
     2041 gcccaccaaa gcaaaaagcc cactggctca cgctaggaac caaaaggccc agcagtgatc
     2101 cagccccaaa agagatctcc tttgccccgg agattacaat ggacgatttc ctctatcttt
     2161 acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc actgataatg
     2221 agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt agagaggcct
     2281 acgcagcaag tctcatcaag acgatctacc cgagtaacaa tctccaggag atcaaatacc
     2341 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc aagaacacag
     2401 agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
     2461 ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cctactgaat
     2521 ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact cgccgtcaag
     2581 actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa aatcttcgtc
     2641 aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa
     2701 gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc
     2761 cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac
     2821 aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt
     2881 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg
     2941 tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc
     3001 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
     3061 cgctacttga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     3121 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     3301 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3361 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     3421 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     3481 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     3541 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac c
//
