LOCUS       sequence-497524-        2512 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH78133
FEATURES             Location/Qualifiers
     source          1..2512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(185..976)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1198..1302)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1370..1388
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1767..1784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1938..2512,1..14))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2018..2037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
       61 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      121 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      181 acagttattt gccgactacc ttggtgatct cgccttttac gtaatggaca aattcttcca
      241 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
      301 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
      361 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
      421 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
      481 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
      541 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
      601 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
      661 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
      721 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
      781 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcgc
      841 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
      901 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
      961 tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     1021 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     1081 cccgaggcat agactgtacc ccaaaaaaaa acagtcataa caagccatga aaaccgccac
     1141 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatact
     1201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     1261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     1321 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     1381 cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcacattgct cgaactcttc
     1441 ctccgttact agcaatagaa gtaatatcaa ggttgttttg ctttctagta acaggaaaag
     1501 aagcagcaga cttaagtcca gtaaaaggag caaccatact agcttgagca gcactagcac
     1561 gagtagcaac aacagcagca gaagaaagca tagaagaagc catgttatcg ataattgtaa
     1621 atgtaattgt aatgttgttt gttgtttgtt gttgttggta attgttgtaa aaatgagctc
     1681 ttatacagta tgagaccaca gagtgattaa tgaatcggcc aacgcgcggg gagaggcggt
     1741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aa
//
