LOCUS       sequence-497525-        2928 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH49477
FEATURES             Location/Qualifiers
     source          1..2928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     130..149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     383..400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1195..1213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1281..1385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1607..2398
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2569..2928,1..229)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
       61 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      241 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      301 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      361 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      421 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaaatg
      481 gggtcatcca agaatgttat caaggagttc atgaggttta aggttcgcat ggaaggaacg
      541 gtcaatgggc acgagtttga aatagaaggc gaaggagagg ggaggccata cgaaggccac
      601 aataccgtaa agcttaaggt aaccaagggg ggacctttgc catttgcttg ggatattttg
      661 tcaccacaat ttcagtatgg aagcaaggta tatgtcaagc accctgccga cataccagac
      721 tataaaaagc tgtcatttcc tgaaggattt aaatgggaaa gggtcatgaa ctttgaagat
      781 ggtggcgtcg ttactgtaac ccaggattcc agtttgcagg atggctgttt catctacaag
      841 gtcaagttca ttggcgtgaa ctttccttcc gatggacctg ttatgcaaaa gaaaacaatg
      901 ggctgggaag ccagcactga gcgtttgtat cctcgtgatg gcgtgttgaa aggagagatt
      961 cataaggctc tgaagctgaa agacggtggt cattacctag ttgaattcaa aagtatttac
     1021 atggcaaaga agcctgtgca gctaccaggg tactactatg ttgactccaa actggatata
     1081 acaagccaca acgaagatta tacaatcgtt gagcagtatg aaagaaccga gggacgccac
     1141 catctgttcc tttaagcttt gagaccacga agtggctctt cagtggacga aagggcctcg
     1201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1381 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     1441 cagtggcggt tttcatggct tgttatgact gttttttttt ggggtacagt ctatgcctcg
     1501 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     1561 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     1621 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     1681 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     1741 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     1801 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     1861 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     1921 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     1981 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     2041 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     2101 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     2161 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     2221 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     2281 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     2341 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
     2401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2701 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgac
//
