LOCUS       sequence-497526-        2738 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44300
FEATURES             Location/Qualifiers
     source          1..2738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      347..935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     836..855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1089..1106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1711..1729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1797..1901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2123..2738,1..176)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
       61 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
      121 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
      181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
      241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
      301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     1081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     1141 tccccgcgcg ttggccgatt cattaatcac tctgtggtct cagcttgctc tcaagatcaa
     1201 aggcttaaaa agctggggtt ttatgaatgg gatcaaagtt tctttttttc ttttatattt
     1261 gcttctccat ttgtttgttt catttccctt tttgttttcg tttctatgat gcacttgtgt
     1321 gtgacaaact ctctgggttt ttacttacgt ctgcgtttca aaaaaaaaaa ccgctttcgt
     1381 tttgcgtttt agtcccattg ttttgtagct ctgagtgatc gaattgatgc ctctttattc
     1441 cttttgttcc ctataatttc tttcaaaact cagaagaaaa accttgaaac tctttgcaat
     1501 gttaatataa gtattgtata agatttttat tgatttggtt attagtctta cttttgctac
     1561 ctccatcttc acttggaact gatattctga atagttaaag cgttacatgt gttccattca
     1621 caaatgaact taaactagca caaagtcaga tattttaaga tcgcaccatt tcgcttgaga
     1681 ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt ttataggtta
     1741 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     1801 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1861 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca cgcaactggt
     1921 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     1981 atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     2041 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     2101 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     2161 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     2221 gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt tgctggttac
     2281 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     2341 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     2401 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     2461 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     2521 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     2581 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     2641 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     2701 ttggtacagc gcagtaaccg gcaaaatcgc gccgaagg
//
