LOCUS       sequence-497527-        2883 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45125
FEATURES             Location/Qualifiers
     source          1..2883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(531..635)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     703..721
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1471..1488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1642..2230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1722..1741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2401..2883,1..309))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
       61 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
      121 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cgctgtgtgg
      181 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
      241 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
      301 ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
      361 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
      421 catagactgt accccaaaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      481 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat actcttcctt
      541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      661 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      721 ccctttcgtc cactgaagag ccacttcgtg gtctcaagta ggatagctag gttacggtga
      781 ggtttttggg tatttataac gtacatatga attgcaaaac ttgaagattt aatttgctta
      841 tctcgtgata tcgtcaatgc cttgatttct attggttgtt gacatttttg agcttagatt
      901 ttttgtcttg tcgagctggc atcagatttt tggtttggaa ttgagttgtg ttagataaat
      961 tagatatctc gtagcatctt agcatattgg aatacccatt gtccattgaa gaggtgccac
     1021 ttgtaaatct gacgtgtttc tatccctttg aatcgtatca gtctcaaggt acacgtcatt
     1081 ggtgtttccc aaatagacat gtcgtcaaag aatcaaaaca acatgataca aagtccaaac
     1141 atacttagaa cgttgcatac tacccactct ttcaccaaaa tgtgaaatat gagtgaaaac
     1201 tgaacatctc aattttcatt actctaagtt tgttaattat cttgttaaaa aaactactct
     1261 aaattacaga agatttaatc ctgataagaa agtacattaa aatgacaaca aagtaaaaaa
     1321 aaaaaataca gttcatcttc aaagacatgt tttctaattc gtattggcaa gaaaaactta
     1381 ccagtcatat ctcctgagac cacagagtga ttaatgaatc ggccaacgcg cggggagagg
     1441 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1501 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1561 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1621 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1681 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1741 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1801 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1861 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1921 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1981 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2041 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2101 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2161 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2221 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2281 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2401 ttatttgccg actaccttgg tgatctcgcc ttttacgtaa tggacaaatt cttccaactg
     2461 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     2521 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     2581 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     2641 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     2701 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     2761 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     2821 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     2881 ctt
//
