LOCUS       sequence-497535-        2940 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH45214
FEATURES             Location/Qualifiers
     source          1..2940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(426..1217)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1439..1543)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1611..1629
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2436..2453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2607..2940,1..255))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2687..2706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
       61 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      421 gacagttatt tgccgactac cttggtgatc tcgcctttta cgtaatggac aaattcttcc
      481 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
      541 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
      601 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
      661 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
      721 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
      781 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
      841 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
      901 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
      961 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     1021 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatcg
     1081 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     1141 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     1201 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
     1261 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
     1321 gcccgaggca tagactgtac cccaaaaaaa aacagtcata acaagccatg aaaaccgcca
     1381 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     1441 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     1501 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1561 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1621 tcacgaggcc ctttcgtcca ctgaagagcc acttcgtggt ctcacattgc ggtgagagtg
     1681 tggcgcaagt aaaaggctct ttagttaata agatgtgatg aaattggata gctaggttac
     1741 ggtgaggttt ttgggtattt ataacgtaca tatgaattgc aaaacttgaa gatttaattt
     1801 gcttatctcg tgatatcgtc aatgccttga tttctattgg ttgttgacat ttttgagctt
     1861 agattttttg tcttgtcgag ctggcatcag atttttggtt tggaattgag ttgtgttaga
     1921 taaattagat atctcgtagc atcttagcat attggaatac ccattgtcca ttgaagaggt
     1981 gccacttgta aatctgacgt gtttctatcc ctttgaatcg tatcagtctc aaggtacacg
     2041 tcattggtgt ttcccaaata gacatgtcgt caaagaatca aaacaacatg atacaaagtc
     2101 caaacatact tagaacgttg catactaccc actctttcac caaaatgtga aatatgagtg
     2161 aaaactgaac atctcaattt tcattactct aagtttgtta attatcttgt taaaaaaact
     2221 actctaaatt acagaagatt taatcctgat aagaaagtac attaaaatga caacaaagta
     2281 aaaaaaaaaa atacagttca tcttcaaaga catgttttct aattcgtatt ggcaagaaaa
     2341 acttaccagt catatctcct gagaccacag agtgattaat gaatcggcca acgcgcgggg
     2401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
//
