LOCUS       sequence-497537-        2379 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM5331
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             56..847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1018..1606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1507..1526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1760..1777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1955..1975
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(2023..2041)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2109..2213
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
       61 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
      121 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
      181 gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
      241 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
      301 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
      361 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
      421 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
      481 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
      541 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
      601 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
      661 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
      721 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
      781 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
      841 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
      901 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
      961 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1021 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1081 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1141 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1201 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1261 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1321 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1381 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1441 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1501 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1561 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1621 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1681 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1741 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     1801 caaaccgcct ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcatactgta
     1861 taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     1921 catttacaat tatcgataca tggcttctag cccaccgaag aagaagcgga aggtcagctg
     1981 gaaaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     2041 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     2101 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     2221 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2281 tcatggcttg ttatgactgt ttttttttgg ggtacagtct atgcctcggg catccaagca
     2341 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatg
//
