LOCUS       sequence-497538-        3903 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL80001
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..75)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     complement(1683..1701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1769..1873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2095..2886
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3057..3645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3546..3565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3799..3816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(3894..3903,1..1643)
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
ORIGIN
        1 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
       61 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
      121 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
      181 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
      241 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
      301 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
      361 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
      421 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
      481 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
      541 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
      601 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
      661 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
      721 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
      781 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
      841 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
      901 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
      961 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     1021 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     1081 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     1141 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     1201 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     1261 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     1321 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     1381 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     1441 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     1501 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     1561 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     1621 agggcggaaa gtccaaattg taagctttga gaccacgaag tggctcttca gtggacgaaa
     1681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1861 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1921 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     1981 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     2041 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     2101 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2161 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2221 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     2281 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     2341 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2401 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     2461 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     2521 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     2581 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     2641 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     2701 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     2761 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     2821 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     2881 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2941 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3001 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3061 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3181 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3841 aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tctgtggtct caaatggagg
     3901 acg
//
