LOCUS       sequence-497540-        2659 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH77911
FEATURES             Location/Qualifiers
     source          1..2659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      420..1008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     909..928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1162..1179
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1705..1723)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1791..1895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2117..2659,1..249)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
       61 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
      121 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
      181 cgcgcagatc agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc
      241 ggcaaataac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1081 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1141 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1201 cgcaaaccgc ctctccccgc gcgttggccg attcattaat cactctgtgg tctcagcttg
     1261 aattaacaga ggtggatgga cagacccgtt cttacaccgg actgggcgcg ggataggata
     1321 ttcagattgg gatgggattg agcttaaagc cggcgctgac accatgctca aggtaggcaa
     1381 tgtcctcagc gtcgagcccg gcatctatgt cgagggcatt ggtggagcgc gcttcgggga
     1441 taccgtgctt gtaactgaga gcggatatga ggccctcact ccgcttgatc ttggcaaaga
     1501 tatttgacgc atttattagt atgtgttaat tttcatttgc agtgcagtat tttctattcg
     1561 atctttatgt aattcgttac aattaataaa tattcaaatc agattattga ctgtcatttg
     1621 tatcaaatcg tgtttaatgg atatttttat tataatattg atgatcgctt gagaccacga
     1681 agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1741 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1801 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1861 gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac tggtccagaa
     1921 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1981 gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2041 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     2101 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     2161 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     2221 ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg ttacggtgac
     2281 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     2341 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     2401 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     2461 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     2521 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     2581 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     2641 gccgcccgac tgggctggc
//
