LOCUS       sequence-497543-        3103 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH87655
FEATURES             Location/Qualifiers
     source          1..3103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    565..620
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     primer_bind     complement(660..678)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        746..850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1072..1863
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2034..2622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2523..2542
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2776..2793
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atggtcaata caggtgctaa ttaagatggt aataatttct tatgtttctg tggtaaagtt
       61 tgattcaatt ccgtagtttt agataatctt atttccatac ataaatttta tagttttatc
      121 tactttgttc ttatgtttta tctctagcca agagttatta ttattatcag aagaagaaaa
      181 aaaaaagaag catatataca aaaggtttaa taaaatgtat tatacaaggc aattatccaa
      241 attttttttg ttttggttta cattgatgct ctcaggattt cataaggata gagagatcta
      301 ttcgtatacg tgtcacgtca tgagtgggtg tttcgccaat ccatgaaacg cacctagata
      361 tctaaaacac atatcaattg cgaatctgcg aagtgcgagc cattaaccac gtaagcaaac
      421 aaacaatcta aaccccaaaa aaaatctatg actagccaat agcaacctca gagattgata
      481 tttcaagata agacagtatt tagatttctg tattatatat agcgaaaatc gcatcaatac
      541 caaaccacct actgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
      601 caattactat ttacaattac aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg
      661 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      841 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      901 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg
      961 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1021 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     1081 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1141 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1201 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1261 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1321 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1381 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1441 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1501 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1561 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1621 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1681 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1741 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     1801 gatcagttgg aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa
     1861 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2161 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     2221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2401 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2521 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2581 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2701 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2761 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2821 ccgcctctcc ccgcgcgttg gccgattcat taatcactct gtggtctcag gagtttccgg
     2881 ttgaaatggg tttggtgaat atccggttca gttatatggt ggccgttgga attggcttat
     2941 tagttgtggc cgttgttgaa gccgttggta ttggtaaggg agaagcagac ttgtggctat
     3001 gagtctatga ccatgactcg tgattatgga gctgtcttat gaccctgacc atcaccttga
     3061 tctggtggat tccaatgttt tcttcttctt ctaataaaat att
//
