LOCUS       sequence-497544-        2346 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44222
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(185..289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     357..375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(588..605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(759..1347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(839..858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1518..2309)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
       61 ttggatgccc gaggcataga ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa
      121 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
      181 gcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      361 ggcgtatcac gaggcccttt cgtccactga agagccactt cgtggtctca cattggaatt
      421 acaaagaaaa gattaagaaa gagaggtttt caaagggtag ttttacaggg gaggcaggga
      481 ttgaaccgta cgctcttgta aataaacagt atgagaccac agagtgatta atgaatcggc
      541 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      601 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      661 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      721 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      781 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      841 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      901 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      961 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1021 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1081 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1141 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1201 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1261 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1321 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1381 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1441 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1501 taaacttggt ctgacagtta tttgccgact accttggtga tctcgccttt tacgtaatgg
     1561 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     1621 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     1681 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     1741 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     1801 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     1861 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     1921 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     1981 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     2041 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     2101 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     2161 aaatcaatat cgctgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     2221 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     2281 gatacttcgg cgatcaccgc ttccctcatg atgtttaact ttgttttagg gcgactgccc
     2341 tgctgc
//
