LOCUS       sequence-497546-        4266 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH75111
FEATURES             Location/Qualifiers
     source          1..4266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(379..483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     551..569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2702..2719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2873..3461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2953..2972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3632..4266,1..157))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 taaccagcaa atcaatatcg ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
       61 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
      121 gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc
      181 gactgccctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt
      241 aacgcgcttg ctgcttggat gcccgaggca tagactgtac cccaaaaaaa aacagtcata
      301 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      361 ccagttgcgt gagcgcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
      481 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
      541 aacctataaa aataggcgta tcacgaggcc ctttcgtcca ctgaagagcc acttcgtggt
      601 ctcaaagctc attgtttgcc tccctgctgc ggtttttcac cgaagttcat gccagtccag
      661 cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt cgcgagtgaa gatccctttc
      721 ttgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa attccatacc
      781 tgttcaccga cgacggcgct gacgcgatca aagacgcggt gatacatatc cagccatgca
      841 cactgatact cttcactcca catgtcggtg tacattgagt gcagcccggc taacgtatcc
      901 acgccgtatt cggtgatgat aatcggctga tgcagtttct cctgccaggc cagaagttct
      961 ttttccagta ccttctctgc cgtttccaaa tcgccgcttt ggacatacca tccgtaataa
     1021 cggttcaggc acagcacatc aaagagatcg ctgatggtat cggtgtgagc gtcgcagaac
     1081 attacattga cgcaggtgat cggacgcgtc gggtcgagtt tacgcgttgc ttccgccagt
     1141 ggcgcgaaat attcccgtgc accttgcgga cgggtatccg gttcgttggc aatactccac
     1201 atcaccacgc ttgggtggtt tttgtcacgc gctatcagct ctttaatcgc ctgtaagtgc
     1261 gcttgctgag tttccccgtt gactgcctct tcgctgtaca gttctttcgg cttgttgccc
     1321 gcttcgaaac caatgcctaa agagaggtta aagccgacag cagcagtttc atcaatcacc
     1381 acgatgccat gttcatctgc ccagtcgagc atctcttcag cgtaagggta atgcgaggta
     1441 cggtaggagt tggccccaat ccagtccatt aatgcgtggt cgtgcaccat cagcacgtta
     1501 tcgaatcctt tgccacgcaa gtccgcatct tcatgacgac caaagccagt aaagtagaac
     1561 ggtttgtggt taatcaggaa ctgttcgccc ttcactgcca ctgaccggat gccgacgcga
     1621 agcgggtaga tatcacactc tgtctggctt ttggctgtga cgcacagttc atagagataa
     1681 ccttcacccg gttgccagag gtgcggattc accacttgca aagtcccgct agtgccttgt
     1741 ccagttgcaa ccacctgttg atccgcatca cgcagttcaa cgctgacatc accattggcc
     1801 accacctgta aggggaaaaa aattgacaaa aagcaaaggt caagcaaatg tatggaaata
     1861 taaaatttca cacagtatct cttaagttac acgttgaagc atgaagtacc tgccagtcaa
     1921 cagacgcgtg gttacagtct tgcgcgacat gcgtcaccac ggtgatatcg tccacccagg
     1981 tgttcggcgt ggtgtagagc attacgctgc gatggattcc ggcatagtta aagaaatcat
     2041 ggaagtaaga ctgctttttc ttgccgtttt cgtcggtaat caccattccc ggcgggatag
     2101 tctgccagtt cagttcgttg ttcacacaaa cggtgatacg tacacttttc ccggcaataa
     2161 catacggcgt gacatcggct tcaaatggcg tatagccgcc ctgatgctcc atcacttcct
     2221 gattattgac ccacactttg ccgtaatgag tgaccgcatc gaaacgcagc acgatacgct
     2281 ggcctgccca acctgaaaaa agattattag cgtcagtaca aatagtatac ttcaaaaaat
     2341 taaaaataca gaccaaaaaa gagagagtaa gacttacctt tcggtataaa gacttcgcgc
     2401 tgataccaga cgttgcccgc ataattacga atatctgcat cggcgaactg atcgttaaaa
     2461 ctgcctggca cagcaattgc ccggctttct tgtaacgcgc tttcccacca acgctgatca
     2521 attccacagt tttcgcgatc cagactgaat gcccacaggc cgtcgagttt tttgatttca
     2581 cgggttgggg tttctacagg acgtaacata agggactgac ccatttgaga ccacagagtg
     2641 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2701 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2761 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2821 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2881 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2941 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3001 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3061 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3121 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3181 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3241 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3301 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3361 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3421 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3481 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3541 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3601 aaagtatata tgagtaaact tggtctgaca gttatttgcc gactaccttg gtgatctcgc
     3661 cttttacgta atggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     3721 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     3781 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     3841 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     3901 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     3961 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     4021 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     4081 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     4141 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     4201 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     4261 tcaccg
//
