LOCUS       sequence-497547-        3413 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL70005
FEATURES             Location/Qualifiers
     source          1..3413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      350..938
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     839..858
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1092..1109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1264..1442
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1296..1478
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      2078..2330
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2389..2407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2475..2579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2801..3413,1..179)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
       61 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
      121 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
      181 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
      241 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
      301 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      361 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      421 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      481 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      541 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      601 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      661 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      721 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      781 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      841 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      901 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      961 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1021 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1081 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1141 ctctccccgc gcgttggccg attcattaat cactctgtgg tctcaggagc ggagaattaa
     1201 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     1261 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     1321 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     1381 gcaaatattt cttgtcaaaa tgctccactg acgttccata aattcccctc ggtatccaat
     1441 tagagtctca tattcactct caatccaaat aatctgcacc ggatctggat cgtttcgcat
     1501 gtctcctgaa agaaggcctg ctgatattag aagggctact gaggctgata tgcctgctgt
     1561 gtgcactatt gtgaatcatt acattgagac ttctactgtg aatttcagga ctgagcctca
     1621 agaaccacaa gagtggactg atgatcttgt gaggcttaga gagaggtatc cttggcttgt
     1681 tgctgaagtg gatggtgaag tggctggtat tgcttatgct ggtccttgga aggctaggaa
     1741 tgcttacgat tggactgctg agtctactgt ttacgtgtct cctaggcatc aaagaactgg
     1801 tcttggatct actctttaca ctcatctttt gaagtctttg gaggctcagg gtttcaagtc
     1861 tgtggttgct gtgattggac ttcctaatga tccttctgtg aggatgcatg aggctcttgg
     1921 ttatgctcct aggggaatgc ttagagctgc tggtttcaaa catggaaatt ggcatgatgt
     1981 gggattctgg cagcttgatt tctctcttcc tgttcctcct agacctgttc ttcctgtgac
     2041 tgagatttga gcgggactct ggatctagag tcaagcagat cgttcaaaca tttggcaata
     2101 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     2161 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     2221 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     2281 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gacgcttgct
     2341 gaattggagc gcttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
     2401 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2461 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2521 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2581 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     2641 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     2701 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     2761 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     2821 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     2881 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     2941 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     3001 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     3061 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     3121 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     3181 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     3241 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     3301 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     3361 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cga
//
