LOCUS       sequence-497549-        3040 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH50581
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     550..567
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1474..1492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1560..1664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1886..2677
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2848..3040,1..396)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
       61 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      481 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      541 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      601 ctccccgcgc gttggccgat tcattaatca ctctgtggtc tcaggagtcg acaaaattta
      661 gaacgaactt aattatgatc tcaaatacat tgatacatat ctcatctaga tctaggttat
      721 cattatgtaa gaaagttttg acgaatatgg cacgacaaaa tggctagact cgatgtaatt
      781 ggtatctcaa ctcaacatta tacttatacc aaacattagt tagacaaaat ttaaacaact
      841 attttttatg tatgcaagag tcagcatatg tataattgat tcagaatcgt tttgacgagt
      901 tcggatgtag tagtagccat tatttaatgt acatactaat cgtgaatagt gaatatgatg
      961 aaacattgta tcttattgta taaatatcca taaacacatc atgaaagaca ctttctttca
     1021 cggtctgaat taattatgat acaattctaa tagaaaacga attaaattac gttgaattgt
     1081 atgaaatcta attgaacaag ccaaccacga cgacgactaa cgttgcctgg attgactcgg
     1141 tttaagttaa ccactaaaaa aacggagctg tcatgtaaca cgcggatcga gcaggtcaca
     1201 gtcatgaagc catcaaagca aaagaactaa tccaagggct gagatgatta attagtttaa
     1261 aaattagtta acacgaggga aaaggctgtc tgacagccag gtcacgttat ctttacctgt
     1321 ggtcgaaatg attcgtgtct gtcgatttta attatttttt tgaaaggccg aaaataaagt
     1381 tgtaagagat aaacccgcct atataaattc atatattttc ctctccgctt tgaatacttg
     1441 agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg
     1501 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1561 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1621 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact
     1681 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1741 gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     1801 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     1861 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca
     1921 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     1981 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt
     2041 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     2101 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     2161 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     2221 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     2281 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     2341 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     2401 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     2461 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     2521 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     2581 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta
     2641 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata
     2701 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2761 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2821 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2881 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2941 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3001 agtgtagccg tagttaggcc accacttcaa gaactctgta
//
