LOCUS       sequence-497551-        3122 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71292
FEATURES             Location/Qualifiers
     source          1..3122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     255..274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     508..525
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1514..1532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1600..1704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1926..2717
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2888..3122,1..354)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
       61 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      121 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      181 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      241 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      301 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      361 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      421 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      481 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      541 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact ctgtggtctc
      601 aggagttaat caaatcaact ttaaaaagcg taacacggaa aacaaaagca aacaaagtat
      661 ataaaatatt aaaatgtata ttgtccgtat ttatgtgtta aattttttct aaaaattcaa
      721 ccaactagta gtgcccaaaa ctgaaaatga tacatccaga aaaatatgga ccttctgcag
      781 caaggaatgg tctgtcttaa gtccaatcca aaaaagactg caatatgagg ctaaactaca
      841 ctcttaaata catggaaaaa ttaaccaaca atgcactatc acacattcat atcttggatg
      901 agataagatt caccatattc cgacacgtgg caccttcatt cttgtctatt actcactcac
      961 tctggttaaa aaggatgatc tagtttgact tgaaacgtag tttgagaaac gaaagaaaac
     1021 tttttgattt tgtgatttta aattaaagtt atgtcaaata tatcaaaatg tccttcaatc
     1081 ttgtggtctt aaatatgtca cgtgaaaaat taaaattaaa gtgttatcag aaaaggaaag
     1141 agatcatttt tttgaaacag aataaaaaaa atagagacat ttttttttta aatagagggc
     1201 gtaaaatatt ttttaaaaaa aggcacttag ctccaatttc ttacctttca tgtggccatt
     1261 aaactttgta atatatcaac aaccaaatcc aatggtcgcc cttcatctaa gatgaggctt
     1321 cttttgtttc tatccgttag attttaaaaa acgtctaaaa ccttatcatt atataaaggg
     1381 acgataccac tgtgtaataa gcatctttaa aaaaaatata gtttctttga aattaaaaaa
     1441 aaaaaaaaca ttttatagct aagtaagtaa acgcaatgtg agaccacgaa gtggctcttc
     1501 agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     1561 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     1621 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     1681 caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa
     1741 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg
     1801 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1861 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     1921 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1981 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2041 atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg
     2101 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2161 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2221 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2281 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2341 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2401 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2461 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2521 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2581 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2641 tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca
     2701 aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag attgatttaa
     2761 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3001 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3121 tg
//
