LOCUS       sequence-497555-        2731 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71431
FEATURES             Location/Qualifiers
     source          1..2731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     466..483
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1081..1099)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1167..1271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1493..2284
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2455..2731,1..312)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
       61 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      361 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      421 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      481 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      541 taatcactct gtggtctcag cttaccgcac tgtgtgtggt ttctcaagac caagacagct
      601 aaagcctaaa gtcagagatc taatatgtgt attgttattc atgacaccac agctgccact
      661 tttggtgtta tgatctgttt gtagaagtag gaattctttt ttttctactt aataatagct
      721 taaagagctg tgcaatttgg tctgtatttt ttgtgtattt tgcactcatt atttgtgaac
      781 agtttgagaa ctatttattt tctaagattt gtgcacgtat gaaccacttt tcatctatat
      841 accaccatgt ttattctgca tctatgggat tgagtttgaa tattcgttga tcaacaaagt
      901 tatatttggt ggatactact tgaaggtgca tatactttgt gctcatatat ttagttgata
      961 ttctggattt tgagctggac aaattgatca aggtagtcta atctggtctg gttactaata
     1021 aaactcaaga gatcactgtc tcgcttgaga ccacgaagtg gctcttcagt ggacgaaagg
     1081 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1261 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1321 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat
     1381 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     1441 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     1501 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1561 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1621 gccacacagc gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1681 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     1741 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1801 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1861 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1921 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1981 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2041 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2101 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2161 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     2221 agatcagttg gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa
     2281 ataactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2341 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2521 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2581 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2701 tggcgataag tcgtgtctta ccgggttgga c
//
