LOCUS       sequence-497556-        2448 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH42760
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(164..955)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1177..1281)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1349..1367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1682..1699)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1853..2441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1933..1952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
       61 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttatttg ccgactacct
      181 tggtgatctc gccttttacg taatggacaa attcttccaa ctgatctgcg cgcgaggcca
      241 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
      301 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
      361 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
      421 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
      481 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
      541 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
      601 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
      661 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
      721 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
      781 caagccttac ggtcaccgta accagcaaat caatatcgct gtgtggcttc aggccgccat
      841 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
      901 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt
      961 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     1021 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     1081 caaaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
     1141 ttcggtcaag gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg
     1201 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1261 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     1321 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact
     1381 gaagagccac ttcgtggtct caagtatagg attggggaat ttagggtaac attcataaga
     1441 gtgtcggtgt ggaaaaaccc acactcttaa attaaaagcg tcggtttcat cgggaccccg
     1501 acactcttaa attaagagtg tcggtgcagc aaaacccaca cttttacttc cattaagagt
     1561 gtcggccccg acactcttta attaactgac actcctttga cctcctgaga ccacagagtg
     1621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2281 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatc
//
