LOCUS       sequence-497557-        3591 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICSL13001
FEATURES             Location/Qualifiers
     source          1..3591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..76)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(736..1527)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1749..1853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1921..1939
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        complement(1982..2327)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2019..2039)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(3397..3414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3568..3591,1..565))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
       61 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
      541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
      601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
      661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
      721 aacttggtct gacagttatt tgccgactac cttggtgatc tcgcctttta cgtaatggac
      781 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
      841 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
      901 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
      961 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     1021 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     1081 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     1141 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     1201 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     1261 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     1321 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     1381 atcaatatcg ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     1441 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     1501 tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg
     1561 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     1621 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa aacagtcata acaagccatg
     1681 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     1741 gagcgcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1861 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1921 aataggcgta tcacgaggcc ctttcgtcca ctgaagagcc acttcgtggt ctcaatgggc
     1981 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     2041 gggattgtgc gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgtag
     2101 acgtggttgg aacctcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     2161 gaccactgtc ggcagagaga tcttgaatga tagcctttcc tttatcgcaa tgatggcatt
     2221 tgtaggagcc accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat
     2281 ggaatccgag gaggtttccc gaaattatcc tttgttgaaa agtctcaata gccctttgat
     2341 cttctgagac tgtatctttg acatttttgg agtagaccag agtgtcgtgc tccaccatgt
     2401 tgacgaagat tttcttcttg tcattgagtc gtaaaagact ctgtatgaac tgttcgccag
     2461 tcttgacggc gagttctgtt agatcctcga tttgaatctt agactccatg catggcctta
     2521 gattcagtag gaactacctt tttagagact ccaatctcta ttacttgcct tggtttatga
     2581 agcaagcctt gaatcgtcca tactggaata gtacttctga tcttgagaaa tatgtctttc
     2641 tctgtgttct tgatgcaatt agtcctgaat cttttgactg catctttaac cttcttggga
     2701 aggtatttga tctcctggag attgttactc gggtagatcg tcttgatgag acttgctgcg
     2761 taggcctctc taaccatctg tgggtcagca ttctttctga aattgaagag gctaaccttc
     2821 tcattatcag tggtgaacat agtgtcgtca ccttcacctt cgaacttcct tcctagatcg
     2881 taaagataga ggaaatcgtc cattgtaatc tccggggcaa aggagatctc ttttggggct
     2941 ggatcactgc tgggcctttt ggttcctagc gtgagccagt gggctttttg ctttggtggg
     3001 cttgttaggg ccttagcaaa gctcttgggc ttgagttgag cttctccttt ggggatgaag
     3061 ttcaacctgt ctgtttgctg acttgttgtg tacgcgtcag ctgctgctct tgcctctgta
     3121 atagtggcaa atttcttgtg tgcaactccg ggaacgccgt ttgttgccgc ctttgtacaa
     3181 ccccagtcat cgtatatacc ggcatgtgga ccgttataca caacgtagta gttgatatga
     3241 gggtgttgaa tacccgattc tgctctgaga ggagcaactg tgctgttaag ctcagatttt
     3301 tgtgggattg gaattcctcc tgagaccaca gagtgattaa tgaatcggcc aacgcgcggg
     3361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg a
//
