LOCUS       sequence-497559-        2992 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH71301
FEATURES             Location/Qualifiers
     source          1..2992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(987..1091)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1159..1177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2036..2053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2207..2795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2287..2306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2966..2992,1..765))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gccttttacg taatggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
       61 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
      121 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
      181 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
      241 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
      301 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
      361 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
      421 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
      481 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
      541 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
      601 ggtcaccgta accagcaaat caatatcgct gtgtggcttc aggccgccat ccactgcgga
      661 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
      721 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
      781 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
      841 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaaa
      901 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
      961 gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg aagcatttat
     1021 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1081 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     1141 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact gaagagccac
     1201 ttcgtggtct cacattttgc ttcttctctc tttttttggt tcaatatgaa ccttttgatg
     1261 tccactatcc ttttatataa tgaaatgata aggtttttga tatgttatgt ggttcttgat
     1321 aacattatac aattacttaa tatctacata tgaaaggttg gaattttttt taagtcacca
     1381 caatagaggt gacacgtgta agcacctcgt taatcttatc tcatccaaga tgggggtagg
     1441 aagaggatat gaatgtatga ttgaggttgg ttttgagttt tttttttttt tttttttgag
     1501 tccttcaact tgttatttta attttttttt ggtgggggag gaggggggtt gaaatattta
     1561 tcatatagta gtccaaagta aattgatagc tagagtactt gtttgcttgc ttatattgtc
     1621 ctcaacttta tgtaatacca tgattccaac ttagacactc ttttaagttg taattttcat
     1681 tattttcttt ttttagagtt ttatgttgaa ttcgcataat tttcaatcgg ataatacaag
     1741 aaaaataata ttttagtata attttataca tgaaatttcg ggaaggtagg atatacggat
     1801 tgtttgtcgg atcagagact ttactcgtac ctttgtaact gttgatccca aatacagata
     1861 gtgactccag agttatatat tatacctata gagactaata tgatttagtg ttattaaaat
     1921 tagtattact aattaattgt aatgccccat gaattctcct gagaccacag agtgattaat
     1981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2941 tatatgagta aacttggtct gacagttatt tgccgactac cttggtgatc tc
//
