LOCUS       sequence-497561-        2443 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pAGM5343
FEATURES             Location/Qualifiers
     source          1..2443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(151..942)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1164..1268)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1336..1354
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(1399..1416)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1664..1681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1835..2423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1915..1934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
       61 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      121 aagtatatat gagtaaactt ggtctgacag ttatttgccg actaccttgg tgatctcgcc
      181 ttttacgtaa tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
      241 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
      301 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
      361 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
      421 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
      481 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
      541 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
      601 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
      661 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
      721 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
      781 caccgtaacc agcaaatcaa tatcgctgtg tggcttcagg ccgccatcca ctgcggagcc
      841 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
      901 tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta actttgtttt
      961 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     1021 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaaaacag
     1081 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     1141 ctggaccagt tgcgtgagcg catactcttc ctttttcaat attattgaag catttatcag
     1201 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1261 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     1321 acattaacct ataaaaatag gcgtatcacg aggccctttc gtccactgaa gagccacttc
     1381 gtggtctcac attccgctgt ggtggtggtg gtggtggctg cctgctgacg cgacgtcggg
     1441 cgtgagccag agcaacgcca cgaggagaaa gccacacagg gcggccattt gcttccccat
     1501 gttatcgata attgtaaatg taattgtaat gttgtttgtt gtttgttgtt gttggtaatt
     1561 gttgtaaaaa tgagctctta tacagtatga gaccacagag tgattaatga atcggccaac
     1621 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1681 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1741 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttc
//
