LOCUS       sequence-497563-        2457 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41414
FEATURES             Location/Qualifiers
     source          1..2457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(134..722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(214..233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(893..1684)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1906..2010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2078..2096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    2135..2309
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2420..2437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
       61 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      541 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttatttgc
      901 cgactacctt ggtgatctcg ccttttacgt aatggacaaa ttcttccaac tgatctgcgc
      961 gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg
     1021 gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga
     1081 ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat
     1141 cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata
     1201 gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc
     1261 tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga
     1321 agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg
     1381 gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa
     1441 tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg
     1501 ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca
     1561 ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc
     1621 gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc
     1681 tcatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc
     1741 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1801 actgtacccc aaaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc
     1861 accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatactct tcctttttca
     1921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1981 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2041 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     2101 tcgtccactg aagagccact tcgtggtctc aagcgatctg gattttagta ctggattttg
     2161 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     2221 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     2281 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagaga
     2341 agctgagacc acagagtgat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2401 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgc
//
