LOCUS       sequence-497564-        2948 bp    DNA     circular SYN 15-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 15, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH44157
FEATURES             Location/Qualifiers
     source          1..2948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(403..1194)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1416..1520)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1588..1606
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2421..2438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2592..2948,1..232))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2672..2691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
       61 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      121 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      181 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      241 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      301 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      361 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttatttgc cgactacctt
      421 ggtgatctcg ccttttacgt aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa
      481 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
      541 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
      601 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
      661 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
      721 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
      781 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
      841 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
      901 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
      961 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1021 aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca ggccgccatc
     1081 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1141 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
     1201 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1261 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1321 aaaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     1381 tcggtcaagg ttctggacca gttgcgtgag cgcatactct tcctttttca atattattga
     1441 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1501 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1561 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccactg
     1621 aagagccact tcgtggtctc aagtatctgt tggttttgtc atctttatat agtgggaacc
     1681 gctagaggca actagcccta atgatcaaag gggccataat tcatgataac gtcgttaatt
     1741 gccacaggat taaggaaagg tgttgacacc cttatcgatt tggaccacta ggataagatc
     1801 acgtggcaga aaactccata atcttatctt tctcatgtaa gggtaggagt gaatgtgtat
     1861 ggctactggt ctgcacttga tcggttgctt ttggctcctt tcaattggga ggcgtcaaat
     1921 tgtagatatc ggttaaatac tttttacata tagattaaaa aaatgattaa atttctatta
     1981 ccttatatta acacattcat atttttattt ttccacttga taatcttaaa taataagaat
     2041 ataagtataa tctttgaatt catcccacat tttaaaagta taattaaatc agacagagtt
     2101 cattcaaaat ggtttttaat tgggaaatgt aaactagtat tttgtgttat tattttctat
     2161 gtagtatgta cgatcactag aacttttagt cacataatgg atattaaatt agaaaacaat
     2221 ccaaatgctt gtgaattttc aggttttacg gctaattaat atatttctga tgaacaagag
     2281 gaacccacgg caccgttcct tttggagcac aacgcaattg cacttacggc ccacttaagc
     2341 ctcctgagac cacagagtga ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2941 gcagccac
//
